Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology

Abstract

Dried ginger-aconite decoction (DAD) is a traditional Chinese medicine (TCM) formula that has been extensively used in the treatment of myocardial ischemia reperfusion injury (MI/RI). However, its specific mechanism against MI/RI has not been reported yet. Therefore, this paper studies the potential active components and mechanism of DAD against MI/RI based on network pharmacology and experimental verification. Sixteen active components of DAD were screened according to oral bioavailability and drug similarity indices. Through Cytoscape 3.7.0, a component-target network diagram was drawn, and potential active components of DAD against MI/RI were determined. Protein-protein interaction (PPI) and compound-target-pathway (C-T-P) networks were established through the software to discover the biological processes, core targets and core pathways of DAD against MI/RI. High Performance Liquid Chromatography (HPLC) analysis identified the presence of potentially active core components for network pharmacological prediction in DAD. It was found that DAD might have played a therapeutic role in anti-MI/RI by activating the PI3K/Akt/GSK-3β signaling pathway in order to reduce mitochondrial hypoxia injury and myocardial cell apoptosis. The network pharmacological prediction was validated by Hypoxia/reoxygenation(H/R) model in vitro and ligation model of the ligation of the left anterior descending branch in vivo. It was verified that DAD had activated PI3K/AKT/GSK-3β to reduce myocardial apoptosis and play a therapeutic function in MI/RI.

An Integrated Approach Based on Network Analysis Combined With Experimental Verification Reveals PI3K/Akt/Nrf2 Signaling Is an Important Way for the Anti-Myocardial Ischemia Activity of Yi-Qi-Tong-Luo Capsule

“…Oxidative stress injury is one of the important mechanisms of MI injury ( Xie et al, 2021 ). In order to determine whether YTC affects biochemical enzymes related to oxidative stress, we measured the levels of intracellular lipid peroxidation products (MDA) and antioxidant enzymes (SOD, GSH-Px and CAT).…”

Mechanism of Action of Flavonoids of Oxytropis falcata on the Alleviation of Myocardial Ischemia–Reperfusion Injury

“…Thus, the role of flavonoids of O. falcata were investigated. The analysis of network pharmacology revealed the intricate interactions among drug components, disease targets, signaling pathways, and molecular mechanisms of TCMs [ 11 , 63 ].…”

“…Traditional Chinese medicine (TCM) has been widely used in China for thousands of years, and it has better therapeutic effects on various complicated disorders with few side effects [ 10 ]. Numerous clinical and experimental studies have demonstrated that Chinese medical prescriptions and herbal medicine extracts may play a therapeutic role in MIRI by exerting an anti-inflammatory and anti-apoptotic effect, maintaining calcium homeostasis, providing the clearance of oxygen free radicals, and avoiding platelet aggregation [ 11 , 12 , 13 , 14 ]. In addition, TCM extracts or mixtures possess more effective pharmacological activities than purified compounds due to their multiple components and targets [ 15 ].…”

Total Flavonoids of Drynariae Rhizoma Improve Glucocorticoid-Induced Osteoporosis of Rats: UHPLC-MS-Based Qualitative Analysis, Network Pharmacology Strategy and Pharmacodynamic Validation

“…Furthermore, several ingredients with high pharmacological activities, which can not fulfill the two conditions (OB ≥ 30%, DL ≥ 0.18), were also included according to the results of our previous qualitative analysis or other studies ( 16 ).…”