“…Different techniques were used to detect anti-MAG antibodies in these patients including ELISA or radioimmunoassay with purified MAG, Western blot after electrophoresis of brain or peripheral myelin proteins or of purified MAG, ELISA or overlay immunostaining after chromatography for reactivity with crossreacting peripheral nerve glycolipids, indirect immunohistochemistry on peripheral nerve sections (reviewed in Nobile-Orazio, 2013) and more recently immunofluorescence for reactivity to HNK-1 (Matà et al, 2011). There was some variability in the detection of these antibodies using these different techniques (Nobile-Orazio, et al, 1989;Pestronk, et al, 1994;van den Berg, et al, 1996;Weiss et al, 1999;Jaskowski et al, 2004Jaskowski et al, & 2007Matà et al, 2011) making it difficult a comparison of the results among different laboratories. This might also reflect differences in the specificity (Nobile-Orazio, et al, 1989;Pestronk, et ql., 1994;Fluri, et al, 2003) or affinity (Ogino, et al, 1994) of these antibodies or in their binding capability to the antigen using different substrate.…”