Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata

Prince, Karina Andrade de; Sordi, Renata; Pavan, Fernando Rogério; Santos, Adolfo Carlos Barreto; Araújo, Ângela R.; Leite, Sergio R. A.; Leite, Clarice Q. F.

doi:10.1590/s1517-83822012000100024

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…The selective index value is used to distinguish toxicity that is due to plant extracts and toxicity in general (Adamu et al, 2014;Prince et al, 2012). Ficus sur showed the highest selectivity index of 3.363 in this study; which means it is three times more effective against M .tuberculosis as compared to human macrophage U937 cells.…”

Section: Cytotoxicity On U937 Cell Line and Selectivitymentioning

confidence: 68%

Inhibition of mycothione disulphide reductase and mycobacterial biofilm by selected South African plants

Oosthuizen

Gasa

Hamilton

et al. 2019

South African Journal of Botany

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Highlights • Antimycobacterial activity of plants traditionally used for the treatment of tuberculosis and its symptoms. • Biofilm inhibition of Mycobacterium sp. and the selectivity towards biofilm inhibition by Eucomis vandermwerwei Verd. and Faurea saligna Harv. • Inhibition of the bacterial mycothiol disulphide reductase by Faurea saligna and its selectivity towards the bacterial analogue vs the human analogue.

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Section: Cytotoxicity On U937 Cell Line and Selectivitymentioning

confidence: 68%

Inhibition of mycothione disulphide reductase and mycobacterial biofilm by selected South African plants

Oosthuizen

Gasa

Hamilton

et al. 2019

South African Journal of Botany

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“…An example of such bioactives can be found in the endophytic fungus Phomopsis stipata, isolated from Styrax camporum Pohl (Styracaceae), in whichits secondary metabolites showed some promising results, with significant in vitro antimycobacterial activity of 31.25 μg mL -1 (Prince et al 2012). In our continuous screening for biologically active secondary metabolites from plant endophytes, we investigated the ones produced by A. fumigatusisolated from Copaifera multijuga, an important Amazonian medicinal plant.…”

Section: Sandoval-montemayormentioning

confidence: 99%

Metabolites from endophytic Aspergillus fumigatus and their in vitro effect against the causal agent of tuberculosis

Silva

Ogusku

et al. 2018

Acta Amaz.

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Tuberculosis (TB) remains one of the most deadly communicable infectious diseases, causing 1.4 million deaths in 2015 worldwide due to many conditions, including the inadequate treatment and the emergence of multidrug-resistant strains of the causal agent, Mycobacterium tuberculosis. Therefore, drugs developed from natural sources, as microorganisms and plant extracts, are a frequent target for the research and discovery of antimicrobial compounds. The current study started the characterization of compounds produced by an Aspergillus fumigatus isolated from copaíba (Copaifera multijuga) that efficiently inhibits M. tuberculosis by releasing the compounds into the fermentation broth under specific culture conditions. A preliminary assay was carried out with a correlate species, M. smegmatis, aiming to detect an antimicrobial effect related to A. fumigatus fermentation broth. The direct use of this substrate in antibiosis assays againstM. tuberculosis H 37 Rv strain (ATCC 27294) allowed the detection of antimicrobial activity with a minimal inhibitory concentration of 256 μg mL -1 , demonstrating that purification processes developed by the Biotage Flash Chromatography System are robust and reliable techniques for purification of compounds from natural sources. Also, this chromatographic system can be used in combination with specific biochemical tests, improving the search for reliable results. We conclude that this fraction can express a broad action range, inhibiting both Mycobacterium species used as target organisms.KEYWORDS: Mycobacterium spp., antimicrobial activity, copaíba, chromatography Metabolitos de Aspergillus fumigatus endofítico e seu efeito in vitro contra o agente causal da tuberculose RESUMO A tuberculose continua a ser uma das doenças infecciosas transmissíveis mais mortais, causando 1,4 milhão de mortes em 2015 em todo o mundo devido a vários fatores, incluindo o tratamento inadequado e o surgimento de cepas multirresistentes do agente causal, Mycobacterium tuberculosis. Portanto, as drogas desenvolvidas a partir de fontes naturais, como micro-organismos e extratos de plantas, são um alvo freqüente para a pesquisa e descoberta de compostos antimicrobianos. O presente estudo foi um ponto de partida para caracterizar compostos produzidos por um Aspergillus fumigatus isolado de copaíba (Copaifera multijuga) que inibe eficientemente M. tuberculosis, liberando os compostos no caldo de fermentação em condições de cultura específicas. Realizou-se um ensaio preliminar com uma espécie correlata, M. smegmatis, com o objetivo de detectar um efeito antimicrobiano relacionado ao caldo de fermentação de A. fumigatus. O uso direto deste substrato em ensaios de antibiose contra a estirpe H37Rv de M. tuberculosis (ATCC 27294) permitiu a detecção de atividade antimicrobiana com uma concentração inibitória mínima de 256 μg mL -1 , demonstrando que os processos de purificação desenvolvidos pelo Biotage Flash Chromatography System são técnicas robustas e confiáveis para purificar compostos de fontes nat...

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“…The MIC values of MM and MIP, nonpathogenic microorganisms obtained from the studied fungal extracts closely related to the sensitivity of Mycobacterium tuberculosis in other studies on fungal extracts. De Prince et al (2012) and Gamboa-Angulo et al (2013) studied extracts from various fungal species for antimycobacterial activity and found them to possess anti-Mycobacterium tuberculosis activity. Jouda et al (2016) isolated various compounds produced by an endophytic fungus of Penicillium species harbored in Garcinia nobilis, and were found to be active against Mycobacterium smegmatis.…”

Section: Cytotoxic Activitiesmentioning

confidence: 99%

Antimycobacterial and cytotoxic activities of extracts from fungal isolates of Lake Magadi

Kowanga¹,

Munissi²,

Masalu³

et al. 2018

Int. J. Bio. Chem. Sci

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In this study, antimycobacterial and cytotoxic activities of ethyl acetate extracts of fungal isolates from Lake Magadi were evaluated. The extracts were tested against Mycobacterium madagascariense (MM) and M. indicus pranii (MIP), and cytotoxicity against brine shrimp (Artemia salina) larvae. Fungal strains were identified using sequence comparison of the Internal Transcribed Spacer (ITS) region. Potent antimycobacterial activities against MM were exhibited by extracts from Volutella colletotrichoides, Helicoon richonis, Penicillium limosum, P. sacculum, Aspergillus parasiticus and A. nomius strains that exhibited minimum inhibition concentration (MIC) in the range of 0.19 -12.5 mg/mL. On the other hand, significant antimycobacterial effects against MIP was shown by extracts from V. colletotrichoides, H. richonis, A. parasiticus, Fusarium merismoides, A. silvaticus and A. fumigatus strains in the same MIC range. Notable cytotoxic activities of the extracts were from A. versicolor, A. nomius, P. janthinellum and H. richonis strains with LC 50 values ranging from 46.60 -98.12 μg/mL. These results indicate that fungi inhabiting Lake Magadi have the ability to produce bioactive metabolites that could be further explored for potential medicinal agents.

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Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata

Cited by 5 publications

References 7 publications

Inhibition of mycothione disulphide reductase and mycobacterial biofilm by selected South African plants

Inhibition of mycothione disulphide reductase and mycobacterial biofilm by selected South African plants

Metabolites from endophytic Aspergillus fumigatus and their in vitro effect against the causal agent of tuberculosis

Antimycobacterial and cytotoxic activities of extracts from fungal isolates of Lake Magadi

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