Anti-mycobacterial, Antimicrobial and Phytochemical Evaluation of Pulicaria crispa and Scoparia dulcis Plant Extracts

Adedayo, Adebiyi; Bassey, Effa Emmanuel; Ayo, Rachael Gbekele-Oluwa; Bello, Isaac Asusheyi; Habila, James D.; Ishaku, Gali Adamu

doi:10.9734/jamps/2016/25199

Cited by 8 publications

(7 citation statements)

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“…A plethora of essential molecules in plants is responsible for both their nutritional, energy giving and prophylactic effects [21]. The mode of action of different complex plant products bear a resemblance to ligands, hormones, signal transduction molecules, etc thus have beneficial medicinal effects on humans due to their potential target sites similarities.…”

Section: Discussionmentioning

confidence: 99%

“…Whole plants, herbs and vegetables provide established launch-pads through the field of phyto-chemistry and pharmacognosy for the development of new drugs and their intermediates that have adequate therapeutic uses [20][21]. A plethora of essential molecules in plants is responsible for both their nutritional, energy giving and prophylactic effects [21].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial and Physicochemical Evaluation of <i>Luffa acutangular</i> Leaf Extracts

Valerie¹

2017

BMB

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Leaves of Luffa acutangular are consumed in some parts of Nigeria as part of folk medicine for the treatment of diseases and as a vegetable food source. This study was undertaken to evaluate the phytochemical constituents from ethanol extracts, antimicrobial resistance, proximate and mineral analysis of its leaf extracts. The phytochemical investigation revealed the presence of alkaloids, phenols, saponins, tannins, terpenoids, and triterpenoids. The elemental analysis of the dried leaf revealed the presence of Calcium (58.6 mg/g), Copper (0.6 mg/g), Magnesium (12.4 mg/g), Manganese (0.9 mg/g), Zinc (0.6 mg/g), Sodium (14.4 mg/g) and Potassium (143.6 mg/g) respectively. The samples were screened against Staphylococcus aureus, Staphylococcus pneumonia, Streptococcus pyrogens, Klebsciella pneumonia, Candida albicans and Candida tropicalis for their anti-microbial activity using Ciprofloxacin, Streptomycin and Fluconazole as control. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) values were determined. Proximate analysis revealed moisture content of 10.6%, ash 6.3%, crude protein 2.6%, crude fiber 4.0%, fat 5.1% and carbohydrate content of 71.4%. The study showed that the leaf extract of L. acutangular may be used to manage some common diseases caused by the tested organisms. The major antimicrobial activity is tailored to the phyto-constituents. This confirms the folkloric use of the plants in the management of various diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Physicochemical Evaluation of <i>Luffa acutangular</i> Leaf Extracts

Valerie¹

2017

BMB

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“…The extracts of P. crispa have shown anti-inflammatory, as well as immunostimulatory effects. For example, several studies have shown antimicrobial effects for P. crispa extracts against Gram-negative bacteria [ 21 – 23 ], Mycobacteria [ 20 , 21 ], Candida albicans [ 17 ], Schistosoma mansoni [ 24 ], Leishmania [ 25 ], and hepatitis B virus [ 26 ]. On the other hand, extracts from the Pulicaria species have been shown to inhibit neutrophil infiltration and alleviate oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

In VitroStudies on the Immunomodulatory Effects ofPulicaria crispaExtract on Human THP-1 Monocytes

Albrahim

Alnasser

Al-Anazi

et al. 2020

Oxidative Medicine and Cellular Longevity

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Background. Pulicaria crispa (P. crispa) is a plant from the Compositae family that exhibits antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities. Objective. The current study aimed at investigating the immunomodulatory effects of P. crispa extract in lipopolysaccharide- (LPS-) stimulated human monocytic THP-1 cells. Methods. To induce macrophage differentiation, THP-1 cell lines were treated with phorbol-12-myristate 13-acetate, followed by exposure to LPS with or without 50 or 100 μg/ml of P. crispa extract. The following tests were employed to test the immunomodulatory effects of the extract: MTT assay, ELISA, Western blotting analysis, cell migration and phagocytosis assays, and Annexin V staining method. Results. Exposure to 100 μg/ml P. crispa extract significantly reduced THP-1 cell proliferation, migration, and phagocytosis (in LPS-stimulated cells, but not in unstimulated cells). Moreover, the extract alone significantly reduced the rate of THP-1 cell apoptosis, while it increased the rate of late apoptosis. Molecular investigations showed that treatment with P. crispa extract significantly upregulated the expression of ERK1, p-MAPK, P-P38, and Bcl2, while it significantly reduced the expression of ERK5, Bax, NF-κB, P-NF-κB, CCL1, CCL2, CCL5, CCL22, CXCL1, and CXCL10. Conclusion. Pulicaria crispa extract exhibited anti-inflammatory, antiproliferative, antimigratory, and antiphagocytic effects in LPS-stimulated THP-1 cells. Future studies should investigate these mechanisms in animal models with chronic inflammatory diseases.

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“…The pungent, repulsive smell and bitter taste in many plants are usually responsible for repressive ability over the metabolic activities of many microorganism [4,5].The screening of photochemical compounds helps in knowing the presence of bioactive compounds which can also be used for antimicrobials analysis of the plant extracts. The phytochemistry of plants shows that the extracts can be use for medicinal applications from different localities [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Phytochemicals Screening, Proximate and Elemental Analysis of Anacardium occidentale L. Nuts and Carica papaya Seeds

Abubakar

Chiroma

Umar

et al. 2019

JALSI

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Aims: The aim of this study was comparative analysis of phytochemicals screening, proximate and elemental analysis of Anacardium occidentale L nut and Carica papaya seeds extracts. Study Design: The samples were cleaned, washed with water, dried, and grinded with a laboratory mortar and pestle, packed in an air tight container and stored ready for further analysis. Place and Duration of Study: The research was conducted at the Chevron Biotechnology Centre, Modibbo Adamawa University of Yola, Nigeria which lasted for about three months. Methodology: The nuts were manually separated from fruit pulp, cleaned, washed with water, dried, roasted and grinded with a laboratory mortar and pestle, packed in an air tight container and stored ready for further analysis. Results: The result of Anacardium occidentale L nut phytochemicals screening showed that with Glycosides (-), Tannins (+), flavonoids(+++), Alkaloids(++), Saponins(-) and the proximate composition (%) were as follows: moisture content (9.7 ± 0.2), ash content (3.46±0.3), crude fat (26.79± 0.5), crude protein (14.26 ± 0.01) carbohydrate (by difference) (45.78± 0.01) Calorific (Energy) value 482.00 Kcal. The mineral analysis (mg/100g) revealed that magnesium (3.50 ± 0.01), Manganese (2.00 ± 0.01), Nickel (3.22 ± 0.01) and Cobalt (0.13 ±0.01) while the phytochemical screening for Carica papaya seeds extracts also showed Glycosides (+), Tannins (++), flavonoids(++), Alkaloids(++), Saponins(-) for the moisture content (8.59 ± 0.3), ash content (4.09±0.16), crude fat (15.60± 0.2), crude protein (15.84 ± 0.01) carbohydrate (by difference) (41.63) Calorific (Energy) value 1549.25 Kcal. The mineral analysis (mg/100g) revealed that magnesium (3.02 ± 0.02), Manganese (0.22 ± 0.01), Nickel (3.41 ± 0.01) and Cobalt (0.18 ± 0.01). Conclusion: The study therefore indicates that the Anacardium occidentale L nut and Carica may not provide all the nutrients required by human in the right proportion though it contains an appreciable quantity of some essential nutrients like Fats, Proteins, Carbohydrate and some minerals.

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Anti-mycobacterial, Antimicrobial and Phytochemical Evaluation of Pulicaria crispa and Scoparia dulcis Plant Extracts

Cited by 8 publications

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Antimicrobial and Physicochemical Evaluation of <i>Luffa acutangular</i> Leaf Extracts

Antimicrobial and Physicochemical Evaluation of <i>Luffa acutangular</i> Leaf Extracts

In VitroStudies on the Immunomodulatory Effects ofPulicaria crispaExtract on Human THP-1 Monocytes

Comparative Analysis of Phytochemicals Screening, Proximate and Elemental Analysis of Anacardium occidentale L. Nuts and Carica papaya Seeds

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Anti-mycobacterial, Antimicrobial and Phytochemical Evaluation of Pulicaria crispa and Scoparia dulcis Plant Extracts

Cited by 8 publications

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Antimicrobial and Physicochemical Evaluation of &lt;i&gt;Luffa acutangular&lt;/i&gt; Leaf Extracts

Antimicrobial and Physicochemical Evaluation of &lt;i&gt;Luffa acutangular&lt;/i&gt; Leaf Extracts

In VitroStudies on the Immunomodulatory Effects ofPulicaria crispaExtract on Human THP-1 Monocytes

Comparative Analysis of Phytochemicals Screening, Proximate and Elemental Analysis of Anacardium occidentale L. Nuts and Carica papaya Seeds

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Product

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Antimicrobial and Physicochemical Evaluation of <i>Luffa acutangular</i> Leaf Extracts

Antimicrobial and Physicochemical Evaluation of <i>Luffa acutangular</i> Leaf Extracts