Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis

Grøndahl, Marie Louise; Nielsen, M.E.; Canto, Mariabeatrice Dal; Fadini, Rubens; Rasmussen, I.A.; Westergaard, LG; Kristensen, Stine Gry; Andersen, Claus Yding

doi:10.1016/j.rbmo.2010.12.005

Cited by 63 publications

(55 citation statements)

References 32 publications

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“…Interestingly, in preovulatory follicles containing a mature MII-oocyte cumulus cells from the COC had a significantly higher AMH expression than mural granulosa cells from the same follicle [20] but a lower production of AMH as compared to cumulus cells from follicles containing immature oocytes [21]. The latter observation is in accordance with a lower expression of AMH receptor 2 in COC from mature vs.…”

Section: Commentssupporting

confidence: 65%

“…With respect to this it could be shown that AMH is also expressed in granulosa cells from mature follicles although to a lesser extent than in those from smaller follicles [20]. Interestingly, in preovulatory follicles containing a mature MII-oocyte cumulus cells from the COC had a significantly higher AMH expression than mural granulosa cells from the same follicle [20] but a lower production of AMH as compared to cumulus cells from follicles containing immature oocytes [21].…”

Section: Commentsmentioning

confidence: 90%

See 1 more Smart Citation

Viability of cumulus cells is associated with basal AMH levels in assisted reproduction

Ebner¹,

Shebl²,

Holzer³

et al. 2014

European Journal of Obstetrics & Gynecology and Reproductiv

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Section: Commentssupporting

confidence: 65%

Section: Commentsmentioning

confidence: 90%

Viability of cumulus cells is associated with basal AMH levels in assisted reproduction

Ebner¹,

Shebl²,

Holzer³

et al. 2014

European Journal of Obstetrics & Gynecology and Reproductiv

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“…Due to its high expression levels, Amh is considered a marker of cumulus cells phenotype [52][53][54][55]. However, under both culture systems studied herein, Amh levels were barely detected.…”

Section: Follicle and Oocyte Development Under The Different In Vitromentioning

confidence: 76%

In vitro follicle growth under non-attachment conditions and decreased FSH levels reduces Lhcgr expression in cumulus cells and promotes oocyte developmental competence

Sánchez

Romero

Albuz

et al. 2011

J Assist Reprod Genet

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Purpose The in-vitro environment influences oocyte competence and gene expression in cumulus cells and oocytes. Effects of culturing under non-attachment conditions and varying follicle exposure to FSH were investigated at the mRNA level and on oocyte developmental capacity. Methods Quantitative PCR analysis of Gdf9, Mater, Nmp2 (in oocytes), Lhcgr and Amh (in cumulus cells), and oocyte developmental competence after in vitro follicle culture were evaluated. Results Follicle survival (98.7%) and polar body rate (94%) were similar for all conditions. Estradiol and progesterone production were significantly lower in non-attachment follicles (10-fold and 3-fold, respectively). Under nonattachment conditions, a higher two-cell rate (69.9%) and total blastocyst yield (48.5%) were obtained and, by decreasing FSH levels during culture, Lhcgr transcripts were significantly reduced to levels similar to in-vivo. Levels of oocyte-specific transcripts were not significantly influenced by in-vitro conditions. Conclusion Non-attachment conditions influence follicle steroid secretory capacity and, together with dynamic FSH doses, positively influence cumulus cell gene expression and oocyte developmental competence.

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“…The AMH is secreted by early GCs in the primary and small antral follicles (36). However, AMH expression still exists in CCs of the preovulatory follicle with lower expression in GCs, suggesting that these cells still have the capacity to secrete AMH into the FF at these later stages of folliculogenesis (37). Previous studies have demonstrated a positive correlation between FF AMH concentration andsuccessful oocyte fertilization, suggesting that AMH production and activity is reduced in GCs and CCs from these patients (8,37,38).…”

Section: Discussionmentioning

confidence: 92%

Women with reduced ovarian reserve or advanced maternal age have an altered follicular environment

Pacella

Zander-Fox

Armstrong

et al. 2012

Fertility and Sterility

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Anti-Müllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis

Cited by 63 publications

References 32 publications

Viability of cumulus cells is associated with basal AMH levels in assisted reproduction

Viability of cumulus cells is associated with basal AMH levels in assisted reproduction

In vitro follicle growth under non-attachment conditions and decreased FSH levels reduces Lhcgr expression in cumulus cells and promotes oocyte developmental competence

Women with reduced ovarian reserve or advanced maternal age have an altered follicular environment

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