Anti-Müllerian hormone levels in serum and testes of male dogs: relations with neuter status and bilateral abdominal cryptorchidism

Prapaiwan, N.; Manee-in, Sukanya; Thanawongnuwech, Roongroje; Srisuwatanasagul, Sayamon

doi:10.1016/j.theriogenology.2023.06.015

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…For AMH and KITLG, which possess two isoforms, the opposite result was observed—transcript level was elevated and total protein level was decreased in the undescended testes—while the level of the KLF4 protein did not differ between samples. Elevated serum levels of the AMH protein have been reported in dogs diagnosed with bilateral abdominal cryptorchidism compared to normal dogs ( 16 ). Our study did not confirm this finding in testicular tissue.…”

Section: Discussionmentioning

confidence: 99%

A massive alteration of gene expression in undescended testicles of dogs and the association of KAT6A variants with cryptorchidism

Stachowiak,

Nowacka-Woszuk,

Szabelska-Beresewicz

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Cryptorchidism is the most common form of disorder of sex development in male dogs, but its hereditary predisposition is poorly elucidated. The gonadal transcriptome of nine unilaterally cryptorchid dogs and seven control dogs was analyzed using RNA-seq. Comparison between the scrotal and inguinal gonads of unilateral cryptorchid dogs revealed 8,028 differentially expressed genes (DEGs) (3,377 up-regulated and 4,651 down-regulated). A similar number of DEGs (7,619) was found by comparing the undescended testicles with the descended testicles of the control dogs. The methylation status of the selected DEGs was also analyzed, with three out of nine studied DEGs showing altered patterns. Bioinformatic analysis of the cDNA sequences revealed 20,366 SNP variants, six of which showed significant differences in allelic counts between cryptorchid and control dogs. Validation studies in larger cohorts of cryptorchid ( n = 122) and control ( n = 173) dogs showed that the TT genotype (rs850666472, p.Ala1230Val) and the AA genotype in 3′UTR (16:23716202G>A) in KATA6 , responsible for acetylation of lysine 9 in histone H3, are associated with cryptorchidism ( P = 0.0383). Both the transcript level of KAT6A and H3K9 acetylation were lower in undescended testes, and additionally, the acetylation depended on the genotypes in exon 17 and the 3′UTR. Our study showed that the massive alteration of the transcriptome in undescended testicles is not caused by germinal DNA variants in DEG regulatory sequences but is partly associated with an aberrant DNA methylation and H3K9 acetylation patterns. Moreover, variants of KAT6A can be considered markers associated with the risk of this disorder.

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Section: Discussionmentioning

confidence: 99%

A massive alteration of gene expression in undescended testicles of dogs and the association of KAT6A variants with cryptorchidism

Stachowiak,

Nowacka-Woszuk,

Szabelska-Beresewicz

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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“…AMH is a member of the Transforming Growth Factor-β family that is expressed in the testis of both foetal and adult mammals [27,28]. The level of AMH has been widely used for the determination of gonadal function in both males and females [29] as well as in pathological conditions [19,30]. In the adult, a high expression of AMH in atrophied and dysfunctional testes may resemble that of the immature testis [31].…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemistry was performed to study the localisation of AR and AMH as previously described by the earlier studies in other species [18,19]. Briefly, the antigen retrieval process was carried out by heating sections in citric acid using a microwave oven at 750 W. The primary antibodies used were rabbit polyclonal anti-AR (clone N-20, Santa Cruz Biotech, Dallas, TX, USA, 1:100) and mouse monoclonal AMH (clone B-11, 1:100).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Geographic Variation in Testicular Morphometrics, Androgen Receptor Expression and Anti-Müllerian Hormone Levels in the Intermediate Roundleaf Bats across Distinct Regions in Thailand

Srisuwatanasagul,

Panyaboriban,

Karapan

et al. 2023

Animals

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The hipposideros larvatus (intermediate roundleaf bat) is one of the insectivorous bats which has an agro-ecological role as a controller of the insect population. The reproductive patterns of H. larvatus are intricately linked to its ecological role and survival. An understanding of the testicular morphology can contribute to conservation for this species particularly in areas where its populations might be declining or under threat. However, these bats may also be associated with zoonotic diseases which can have significant public health implications. The aims of the study were to examine the morphological data as well as the expression of the androgen receptor (AR) and anti-Müllerian hormone (AMH) in the male reproductive organs of H. larvatus from different areas of Thailand and at different sampling periods. Their testes were processed for histological investigation and immunohistochemistry for AR and AMH. The results showed differences among the various sampling areas and different sampling periods, which suggested seasonal breeding characteristics. The higher testicular morphometric data were observed in H. larvatus from the Dong Phayayen (DY) and Chiang Dao (CD) areas during June, while the size of seminiferous tubules decreased thereafter. High AR immunostaining was noticed when the testicular morphometric data were higher in DY bats during June. On the other hand, low AR was observed in bats during August and September, which was concomitant with the decreases in seminiferous tubule size and germinal epithelial height. The results suggest a potential correlation between AR immunostaining and the active phase of testicular functions in H. larvatus during June which may imply the involvement of AR with the enhancement of testicular activity. Conversely, the low expression of AR may contribute to the upregulation of AMH in the testes and may indicate lower testicular activity in H. larvatus in Thailand.

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Half-life of serum anti-Müllerian hormone and changes after gonadectomy in adult female and male dogs with normal and abnormal gonads

Balogh,

Szilágyi,

Balogh

et al. 2024

Theriogenology

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Anti-Müllerian hormone levels in serum and testes of male dogs: relations with neuter status and bilateral abdominal cryptorchidism

Cited by 5 publications

References 52 publications

A massive alteration of gene expression in undescended testicles of dogs and the association of KAT6A variants with cryptorchidism

A massive alteration of gene expression in undescended testicles of dogs and the association of KAT6A variants with cryptorchidism

Geographic Variation in Testicular Morphometrics, Androgen Receptor Expression and Anti-Müllerian Hormone Levels in the Intermediate Roundleaf Bats across Distinct Regions in Thailand

Half-life of serum anti-Müllerian hormone and changes after gonadectomy in adult female and male dogs with normal and abnormal gonads

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