Anti–MSP-10 IgG indicates recent exposure to Plasmodium vivax infection in the Peruvian Amazon

Aguirre, Ángel Rosas; Patra, Kailash P.; Calderón, Maritza; Torres, Katherine; Gamboa, Dionicia; Arocutipa, Edith; Málaga, Edith; Garro, Katherine; Fernández, C.F. González; Trompeter, Grace; Alnasser, Yossef; Llanos‐Cuentas, Alejandro; Gilman, Robert H.; Vinetz, Joseph M.

doi:10.1172/jci.insight.130769

Cited by 10 publications

(28 citation statements)

References 50 publications

(39 reference statements)

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“…Thirty-two (89%) reported the methodology used to elute the DBS samples [14,15,[17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][35][36][37][39][40][41][42][44][45][46][47][48][49][50][51][52]. The shortest incubation period of the DBS in elution buffer (50 μl 0.05% PBS/Tween-20) was 30 minutes [42] and the longest incubation period of the DBS in elution buffer (700 μl and 300 μl PBS/0.05% BSA) was 18-24 hours [17]) ( Table 2).…”

Section: Elution Methodsmentioning

confidence: 99%

“…Overall, DBS and serum samples from 16,255 individuals were included: 13,742 (84.5%) adults, 560 (3.4%) 5-to 17-year old's and 2,082 (12.8%) less than five years old. Two studies reported antibodies against hepatitis A [14,15], nine hepatitis B [16][17][18][26][27][28]46,48,51], ten hepatitis C [19][20][21][22][26][27][28]37,46,48], eight HIV [23][24][25][26][27][28]46,50], three human papillomavirus (HPV) [29,30,37], three measles [31,32,42], three rubella [33,34,42], two syphilis [40,46], two H. pylori [35,47] and two malaria [38,49]. Twelve papers reported on Chagas disease [35], Epstein-Barr virus [36], HPV, H. pylori, hepatitis C and polyomavirus [37], Strong...…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Twelve papers reported on Chagas disease [35], Epstein-Barr virus [36], HPV, H. pylori, hepatitis C and polyomavirus [37], Strongyloidiasis [39], pertussis [41], hepatitis E [56], Vibrio cholera [2], measles, mumps and rubella [42], tuberculosis and cytomegalovirus [43], toxoplasmosis [44], trypanosoma [45], Covid-19 [52], respectively. Twelve studies were conducted in Europe [14,18,21,27,29,30,35,[41][42][43]50,52], four in North America [19,20,32,36], eleven in South America [15][16][17]22,24,34,38,39,48,49,51], seven in Africa [23,25,26,31,40,44,45] and five in Asia [28,33,…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Thirty of the studies used enzyme-linked immunosorbent assays (ELISA) [14][15][16][17]19,[21][22][23][24][25][26][29][30][31][32]34,36,38,39,[41][42][43][44][45][46][47][49][50][51][52] with the one study using the Luminex 100 [37] or a combination of Treponema pallidum particle agglutination assay (TPPA), Treponema pallidum hemagglutination assay (TPHA) and ELISA [40]. Two studies used the architect system [27,35] as a detection method and two studies used chemiluminescence immunoassay (CIA) either alone [18] or in combination with ELISA [20].…”

Section: Diagnostic Performancementioning

confidence: 99%

“…Twenty-one studies reported that their DBS samples were stored at -20˚C [17,19,[22][23][24]26,27,30,33,34,36,39,[41][42][43]46,[48][49][50]53], three studies stored at -80˚C [37,38,44], five studies stored at 4˚C [16,31,32,44,51], two studies stored at room temperature [20,35] and in two studies the DBS's were stored at -5˚C to -10˚C [40] and -70˚C [21], respectively (Table 2).…”

Section: Storage Of Dbs Cardsmentioning

confidence: 99%

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Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

et al. 2021

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Background Increasingly, vaccine efficacy studies are being recommended in low-and-middle-income countries (LMIC), yet often facilities are unavailable to take and store infant blood samples correctly. Dried blood spots (DBS), are useful for collecting blood from infants for diagnostic purposes, especially in low-income settings, as the amount of blood required is miniscule and no refrigeration is required. Little is known about their utility for antibody studies in children. This systematic review aims to investigate the correlation of antibody concentrations against infectious diseases in DBS in comparison to serum or plasma samples that might inform their use in vaccine clinical trials. Methods and findings We searched MEDLINE, Embase and the Cochrane library for relevant studies between January 1990 to October 2020 with no language restriction, using PRISMA guidelines, investigating the correlation between antibody concentrations in DBS and serum or plasma samples, and the effect of storage temperature on DBS diagnostic performance. We included 40 studies in this systematic review. The antibody concentration in DBS and serum/plasma samples reported a good pooled correlation, (r2 = 0.86 (ranged 0.43 to 1.00)). Ten studies described a decline of antibody after 28 days at room temperature compared to optimal storage at -20°C, where antibodies were stable for up to 200 days. There were only five studies of anti-bacterial antibodies. Conclusions There is a good correlation between antibody concentrations in DBS and serum/plasma samples, supporting the wider use of DBS in vaccine and sero-epidemiological studies, but there is limited data on anti-bacterial antibodies. The correct storage of DBS is critical and may be a consideration for longer term storage.

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Section: Elution Methodsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Diagnostic Performancementioning

confidence: 99%

Section: Storage Of Dbs Cardsmentioning

confidence: 99%

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Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

et al. 2021

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Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1

et al. 2022

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The Plasmodium ookinete uses chitinase activity to penetrate the acellular, chitin-containing peritrophic matrix to invade the mosquito vector. Plasmodium ookinetes from different parasite clades secrete two structurally distinct forms of chitinase, one, a short form lacking a C-terminal putative chitinbinding domain (CBD), the other, a long form with both proenzyme and C-terminal putative chitin-binding domains. Here, we structurally and functionally characterize the three cysteines in the short chitinase of the humaninfecting malaria parasite, P. falciparum testing the hypothesis that one unpaired cysteine would not contribute to chitinase-specific enzymatic activity which would identify this residue as potentially involved in intermolecular disulfide bonding and heteromultimeric invasion complex formation as previously described. To test this hypothesis, we produced and characterized recombinant wild-type and cysteine-mutation PfCHT1 proteins in E. coli and used biophysical and enzymatic approaches to examine their enzymatic activities and chitin-binding affinities. The cysteine-203 PfCHT1 mutation had no effect on chitinolytic and chitin-binding functions. The cysteine-220 and cysteine-230 mutants were enzymatically inactive and did not bind to chitin. The artificial intelligence-based protein prediction algorithm, AlphaFold, correctly identified the involvement of cys-220 and cys-230 in the intramolecular disulfide linkages key to maintaining properly folded chitinase structural integrity. AlphaFold predicted that cys-203 cysteine is surface exposed and thus involved in intermolecular protein-protein interaction. Production of the cys-to-ser 203 PfCHT1 mutant facilitated recombinant protein production. Future cellular and biochemical studies are needed to further understand details of Plasmodium ookinete mosquito midgut invasion.

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Temporal and Microspatial Heterogeneity in Transmission Dynamics of Coendemic Plasmodium vivax and Plasmodium falciparum in Two Rural Cohort Populations in the Peruvian Amazon

Aguirre

Guzmán-Guzmán

Chuquiyauri

et al. 2020

The Journal of Infectious Diseases

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Background Malaria is highly heterogeneous; its changing malaria micro-epidemiology needs to be addressed to support malaria elimination efforts at the regional level. Methods A three-year, population-based cohort study in two settings in the Peruvian Amazon (Lupuna, Cahuide) followed participants by passive and active case detection from January 2013 to December 2015. Incidence and prevalence rates were estimated using microscopy and PCR. Results Lupuna registered 1,828 infections (1,708 P. vivax, 120 P. falciparum; incidence was 80.7 infections/100 person-years (95%CI [77.1–84.5]). Cahuide detected 1,046 infections (1,024 P. vivax, 20 P. falciparum, two mixed); incidence was 40.2 infections/100 person-years (95%CI [37.9–42.7]). Recurrent P. vivax infections predominated onwards from 2013. According to PCR data, submicroscopic predominated over microscopic infections, especially in periods of low transmission. The integration of parasitological, entomological and environmental observations evidenced an intense and seasonal transmission resilient to standard control measures in Lupuna, and a persistent residual transmission after severe outbreaks were intensively handled in Cahuide. Conclusions In two exemplars of complex local malaria transmission, standard control strategies failed to eliminate submicroscopic and hypnozoite reservoirs, enabling persistent transmission.

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Anti–MSP-10 IgG indicates recent exposure to Plasmodium vivax infection in the Peruvian Amazon

Abstract: Role of funding source: The funding agency (NIH) had no role in the design of this study nor any role in its execution, analyses, and interpretation of data or the decision to submit results.

Cited by 10 publications

References 50 publications

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1

Temporal and Microspatial Heterogeneity in Transmission Dynamics of Coendemic Plasmodium vivax and Plasmodium falciparum in Two Rural Cohort Populations in the Peruvian Amazon

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