Anti-microbial and anti-mycotoxigenic activities of endophytic <i>Alternaria alternata</i> isolated from <i>Catharanthus roseus</i> (L.) G. Don.: molecular characterisation and bioactive compound isolation

Sudharshana, Tungeti Narasimhappa; Venkatesh, Hosur Narayanappa; Nayana, Borah; Manjunath, K.; Mohana, Devihalli Chikkaiah

doi:10.1080/21501203.2018.1541933

Cited by 18 publications

(11 citation statements)

References 30 publications

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“…The gram-positive pathogens were effectively inhibited at lower concentrations by the extract of A. arborescens, F. proliferatum, and P. vismiae culture filtrate. Similar to the previous findings, the ethyl acetate extract of endophytic fungus Alternaria alternata tested against S. aureus, E. coli, K. pneumonia, S. typhi, and P. aeruginosa had shown significant antimicrobial activities at low minimum inhibitory concentration concentrations 7.8-125 µg/ml and zone of inhibition ranging from 12.5 to 24.5 mm [49]. Mishra et a.…”

Section: Gc-ms Analysissupporting

confidence: 85%

Antioxidant, antibacterial, cytotoxicity, and phytochemical potentials of fungal bioactive secondary metabolites

et al. 2022

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The present study focuses on antioxidant, antibacterial, phytochemical potential, and cytotoxicity of secondary metabolites from Colletotrichum gloeosporioides, Fusarium proliferatum, Alternaria arborescens, and Pestalotiopsis vismiae. The scavenging effect of fungal metabolites was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), H 2 O 2 , O 2 -, and reducing power assays.Among the four fungi, A. arborescens have higher antioxidant activity of DPPH (81.12%), H 2 O 2 (74.83%), superoxide (75.74%), and reducing power (0.141) with standard ascorbic acid. Fungal metabolites were found to be significantly effective against all tested pathogens. A. arborescens showed significant amounts of phenolic (138.78 ± 3.70 mg/g of gallic acid equivalent), flavonoid (74.16 ± 3.58 mg/g of quercetin equivalent), as well as cytotoxicity effects in a cancer cell line (A549) with inhibitory concentration (IC 50 = 35 µg/ml at 24 and 48 h). The live and dead cells were analyzed by acridine orange/ethidium bromide staining methods. In addition, A. arborescens metabolites were characterized through gas chromatography-mass spectrometry analysis. The major compounds were found to be phenol, 4-(1,1,3,3-tetramethylbutyl)-, acetamide, N-(3-methylphenyl)-, dibutyl phthalate, and benzo[qr]naphtho [2,1,8,7-fghi] pentacene. The compounds were reported in A. arborescensis up to our knowledge this is the first report. Based on this study, all fungal strains have a significant bioactive capacity with A. arborescens strain exhibiting greater biological properties. Further purification and characterization of A. arborescens bioactive component could lead to an intriguing reservoir of novel pharmacological agents in the future.

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Section: Gc-ms Analysissupporting

confidence: 85%

Antioxidant, antibacterial, cytotoxicity, and phytochemical potentials of fungal bioactive secondary metabolites

et al. 2022

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“…In terms of Alternaria , extracts and isolated compounds obtained from endophytic fungi belonging to this genus were previously reported to possess a broad spectrum of antimicrobial activities [ 25 , 26 ]. In this study, EtOAc extracts of both EL 24 and EL 35 displayed promising inhibitory activity towards three different strains of S. aureus , including, importantly, the two methicillin-resistant strains.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia

Caruso,

Palombo,

Moulton

et al. 2023

Antibiotics

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The threat to public health resulting from the emergence of antimicrobial resistance (AMR) is ever rising. One of the major bacterial pathogens at the forefront of this problem is methicillin-resistant Staphylococcus aureus, or MRSA, for which there is a great need to find alternative treatments. One of the most promising alternatives is endophytic fungi, which were shown to produce a vast array of bioactive compounds, including many novel antibacterial compounds. In this study, two endophytic Alternaria sp., EL 24 and EL 35, were identified from the leaves of Eremophila longifolia. Ethyl acetate (EtOAc) extracts of their culture filtrates were found to inhibit both methicillin-sensitive S. aureus ATCC 25923 and MRSA strains M173525 and M180920. The activity of each extract was shown to be greatly affected by the growth medium, with considerable reductions in minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) observed when tested in tryptic soy broth with glucose (TSBG) compared with Mueller–Hinton broth (MHB). Both extracts displayed significant (p ≤ 0.05) antibiofilm activity against all three S. aureus strains, the greatest of which was that of EL 35, which reduced biofilm formation by M180920 by 72%, while that of EL 24 resulted in a 57% reduction against ATCC 25923. Both extracts also disrupted established biofilms, of which the most effective was EL 35, which reduced the M180920 biofilm by 64%, while EL 24 also performed best against M180920, reducing biofilm by 54%. Gas chromatography–mass spectrometry (GC-MS) analysis of the EL 24 EtOAc extract revealed five known compounds. This study highlights the promise of endophytic fungi from Australian plants as a potential source of substances effective against important bacterial pathogens. Further understanding of the responsible compounds and their mechanisms could lead to the development of treatments effective against MRSA, as well as novel biofilm-resistant biomedical materials, contributing towards reducing the burden of AMR.

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“…The ethyl acetate (EA) extract and the p -coumaric acid obtained from the endophytic fungus A. alternata isolated from C. roseus leaves showed concentration-dependent broad-spectrum antimicrobial activity against E. coli , Klebsiella pneumoniae , P. vulgaris , P. aeruginosa , S. typhimurium , S. aureus , and Streptococcus faecalis , and on the yeast C. albicans and C. neoformans [ 222 ]. These EA extracts and the p -coumaric acid were also effective as antifungal on the phytopathogens A. brassicicola , Alternaria geophila, A. flavus (aflatoxigenic strain), A. fumigatus , Aspergillus ochraceus, A. tamarii, A. terreus, Curvularia tetramera, F. oxysporum, Fusarium lateritium, F. equiseti, Fusarium udum, Fusarium verticillioides (fumonisinogenic strain), P. citrinum , and Penicillium expansum , showing MIC from 7.8 to 250 µg/mL.…”

Section: Studies Of Fungal Secondary Metabolites As Cytotoxic and Ant...mentioning

confidence: 99%

Overview of Bioactive Fungal Secondary Metabolites: Cytotoxic and Antimicrobial Compounds

et al. 2022

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Microorganisms are known as important sources of natural compounds that have been studied and applied for different purposes in distinct areas. Specifically, in the pharmaceutical area, fungi have been explored mainly as sources of antibiotics, antiviral, anti-inflammatory, enzyme inhibitors, hypercholesteremic, antineoplastic/antitumor, immunomodulators, and immunosuppressants agents. However, historically, the high demand for new antimicrobial and antitumor agents has not been sufficiently attended by the drug discovery process, highlighting the relevance of intensifying studies to reach sustainable employment of the huge world biodiversity, including the microorganisms. Therefore, this review describes the main approaches and tools applied in the search for bioactive secondary metabolites, as well as presents several examples of compounds produced by different fungi species with proven pharmacological effects and additional examples of fungal cytotoxic and antimicrobial molecules. The review does not cover all fungal secondary metabolites already described; however, it presents some reports that can be useful at any phase of the drug discovery process, mainly for pharmaceutical applications.

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Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation

Abstract: View related articles View Crossmark data Citing articles: 1 View citing articles Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation

Cited by 18 publications

References 30 publications

Antioxidant, antibacterial, cytotoxicity, and phytochemical potentials of fungal bioactive secondary metabolites

Antioxidant, antibacterial, cytotoxicity, and phytochemical potentials of fungal bioactive secondary metabolites

Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia

Overview of Bioactive Fungal Secondary Metabolites: Cytotoxic and Antimicrobial Compounds

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