Anti-microbial activity of a new vaginal contraceptive NIM-76 from neem oil (Azadirachta indica)

Sairam, M.; Ilavazhagan, G.; Sharma, Shivani; Dhanraj, SA; Suresh, B.; Parida, Manmohan; Jana, Asha Mukul; Kumar, Dileep; Selvamurthy, W.

doi:10.1016/s0378-8741(99)00211-1

Cited by 101 publications

(70 citation statements)

References 11 publications

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“…There are no reports in the literature on the antimicrobial potential of the essential oil of G. kunthiana and studies on antimicrobial testing with essential oils of plants from the family Meliaceae are scarce. Data similar to those found in the present study were reported by Sairam et al (2000) who assessed the antibacterial and antifungal potential of the essential oil of Azadirachta indica (Meliaceae) and found greater antimicrobial activity of the essential oil against Gram-positive bacteria than against Gram-negative bacteria. Aromdee and Sriubolmas (2006) assessed the antimicrobial potential of the mentioned plant and observed moderate antimicrobial activity against B. subtilis and C. albicans.…”

Section: Resultssupporting

confidence: 92%

Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss

et al. 2017

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The essential oils are extracted from plant compounds and can present activities antimicrobial and antioxidant properties. The goals of the present study were: (a) to determine the chemical composition of the essential oil of Guarea kunthiana A. Juss using the method of gas chromatography coupled to mass spectrometry (GC-MS); (b) to evaluate the antimicrobial potential of this oil using the broth microdilution method against different microorganisms: five Gram-negative bacteria, four Gram-positive bacteria and a yeast and (c) to determine the antioxidant activity of the oil using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay. The GC-MS analyses allowed identifying 13 constituents, representing 96.52% of the essencial oil composition. The main compounds identified were α-zingiberene (34.48%), β-sesquiphellandrene (22.90%), and α-curcumene (16.17%). With respect to the antimicrobial activity, the essential oil was effective against all the microorganisms tested, except for the bacteria E. coli and K. pneumoniae, which were resistant to the action of the oil. From a general point of view, Gram-positive bacteria were more susceptible to the action of the essential oil than Gram-negative bacteria. The essential oil exhibited antioxidant potential.Keywords: Guarea kunthiana, essential oil, chemical composition, antimicrobial activity, antioxidant. Composição química, potencial antimicrobiano e antioxidante do óleo essencial de Guarea kunthiana A. Juss ResumoOs óleos essenciais são compostos extraídos de plantas e podem apresentar propriedades antimicrobianas e antioxidantes. O objetivo deste trabalho foi (a) determinar a composição química do óleo essencial de Guarea kunthiana A. Juss pelo método de cromatografia gasosa acoplada à espectrometria de massas (CG-EM); (b) avaliar o potencial antimicrobiano deste óleo pelo método de microdiluição em caldo frente a diferentes micro-organismos, sendo cinco bactérias Gram-negativas, quatro Gram-positivas uma levedura e (c) por fim, determinar a atividade antioxidante do óleo pelo método de captura do radical livre 2,2-difenil-1-picril hidrazil (DPPH). As análises de CG-EM resultaram na identificação de 13 constituintes, representando 96,52% da composição do óleo essencial. Os principais compostos identificados foram α-Zingibereno (34,48%), β-Sesquifelandreno (22,90%) e α-Curcumeno (16,17%). Em relação à atividade antimicrobiana, o óleo essencial foi efetivo frente a todos os micro-organismos testados exceto para as bactérias E. coli e K. pneumoniae, as quais se apresentaram resistentes à ação do óleo. Em geral, as bactérias Gram-positivas foram mais suscetíveis à ação do óleo essencial em relação às Gram-negativas. O óleo essencial apresentou potencial.Palavras-chave: Guarea kunthiana, óleo essencial, composição química, atividade antimicrobiana, antioxidante.

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Section: Resultssupporting

confidence: 92%

Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss

et al. 2017

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“…Efficacy of NIM-76, a spermicidal fraction from neem oil was investigated for its antimicrobial action against certain bacteria, fungi and poliovirus as compared to whole neem oil. This shows that NIM-76 has a potent broad spectrum antimicrobial activity (SaiRam, 2000). Available antimicrobial agents can control the infection but they are expensive and rapid emergence of anti-microbial resistance.…”

Section: Effect Of Neem Oil On Some Pathogenic Bacteriamentioning

confidence: 98%

Effect of neem oil on some pathogenic bacteria

Jahan

Begum

Sultana

2008

Bangladesh J Pharmacol

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“…The extract had stronger antimicrobial activity than the oil and, in vitro at 2 mg/mL, inhibited the growth of Escherichia coli and Klebsiella pneumoniae, which were not inhibited by the oil. The extract was active against Candida albicans (minimum inhibitory concentration 0.25 mg/mL) and had antiviral activity against poliovirus replication in Vero African green monkey kidney cell lines at 50 µg/mL [42] . Intravenous administration of 60 mg/ kg body weight of the oil twice per day for 7 d protected mice from systemic candidiasis, as shown by enhanced survival and a reduction in colony-forming units of Candida albicans in various tissues [42] .…”

Section: Antimicrobial and Antiviral Effectsmentioning

confidence: 99%

“…The extract was active against Candida albicans (minimum inhibitory concentration 0.25 mg/mL) and had antiviral activity against poliovirus replication in Vero African green monkey kidney cell lines at 50 µg/mL [42] . Intravenous administration of 60 mg/ kg body weight of the oil twice per day for 7 d protected mice from systemic candidiasis, as shown by enhanced survival and a reduction in colony-forming units of Candida albicans in various tissues [42] . The oil inhibited the growth of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus pyogenes in vitro at a concentration of 1.5%-6.0% [43] .…”

Section: Antimicrobial and Antiviral Effectsmentioning

confidence: 99%

Review on pharmacological and toxicologyical effects of oleum azadirachti oil

Koriem

2013

Asian Pacific Journal of Tropical Biomedicine

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Anti-microbial activity of a new vaginal contraceptive NIM-76 from neem oil (Azadirachta indica)

Cited by 101 publications

References 11 publications

Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss

Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss

Effect of neem oil on some pathogenic bacteria

Review on pharmacological and toxicologyical effects of oleum azadirachti oil

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