Anti-metastatic Activity of Curcumin Analog Pentagamaboronon-0-Sorbitol Against HER2-overexpressed MCF-7 Breast Cancer Cells

Abstract

Breast cancer with Human Epidermal Growth Factor Receptor (HER)2 overexpression increases tumor progession and lead to metastasis, which is primarily cause of mortality in breast cancer. Pentagamaboronon-0 Sorbitol (PGB-0-So) is an aquoeous formulation of curcumin analog, PGB-0, with sorbitol. This compound has been developed as an anti-cancer chemotherapeutic agent and a boron carrying pharmaceutical for boron neutron capture therapy (BNCT). The aims of this study are to investigate antimetastatic activities of PGB-0-So against HER2-overexpressed MCF-7 breast cancer (MCF-7/HER2) cells. The MTT cytotoxicity assay of PGB-0-So exhibited cytotoxic effect with an IC50 value of 35 μM. The testing of anti-migration activity using the scratch wound healing assay demonstrated that PGB-0-So inhibited the closure of the wound on MCF-7/HER2 cells compare to the control. Furthermore, PGB-0-So was able to suppress matrix metalloproteinase (MMP)-9 activities, based on the gelatin zymography assay. In conclusion, PGB-0-So has potency to be developed as an anti-cancer agent against metastatic breast cancer.Keywords : PGB-0-So, anti-metastatsis, cell migration, MMP-9, MCF-7/HER2

CCA-1.1, a Novel Curcumin Analog, Exerts Cytotoxic anti- Migratory Activity toward TNBC and HER2-Enriched Breast Cancer Cells

“…In chemotherapy development, the selectivity becomes a critical feature, indicating that a drug candidate should exhibit higher effect against cancer cells than normal cells, with SI >2 marked as high selectivity of a candidate drug as the closure percent by using ImageJ software (version 1.51) (Qodria et al, 2018;Ramadani et al, 2018).…”

Pentagamaboronon-0-Sorbitol Induces Apoptosis through Elevation of Reactive Oxygen Species in Triple Negative Breast Cancer Cells

“…Since this compound showed poor solubility in water, complexation PGB-0 with polyol sugar Sorbitol was done to optimize the potency of this substance as anticancer agent (Chen, et al, 2013;Dash, Srinivas and Babu, 2019). PGB-0-So had reported to inhibit the activity of Matrix Metalloproteinase 9 (MMP-9) which plays role in tumor invasion in several breast cancer cells (Qodria, et al, 2018;Ramadani, et al, 2018).…”

Analog Curcumin, Pentagamavunon-0 (PGV-0), Induces Senescence and Increases Cytotoxic Effect of Doxorubicin on HCC 1954 Cells

“…PGB-0 reported to decreased the viability of MCF-7/HER2 cells (Utomo, et al, 2017) and4T1 cells (Kusumastuti, et al, 2019). The PGB-0 in a complex form with sorbitol (PGB-0-So) increased cytotoxicity against MCF-7/HER2 cells (Qodria, et al, 2018). Whereas, PGV-0 is known to have cytotoxic activities in various types of cancer cells, including breast cancer (Hermawan, et al, 2011).…”