Anti-Melanoma Capability of Contactless Cold Atmospheric Plasma Treatment

Yan, Dayun; Wang, Qihui; Malyavko, Alisa; Keidar, Michael

doi:10.3390/ijms222111728

Cited by 6 publications

(13 citation statements)

References 32 publications

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“…Physical factors, likely mainly the EM effect generated in CAP, have several novel features compared with chemical factors such as ROS and RNS in CAP. First, physical factors in CAP can penetrate the dielectric barrier (~1 mm) and air gap (~8 mm) to affect cells, which is consistent with the widely observed non-invasive nature of the transdermal capability of CAP treatment on the skin above tumor tissues [36,91]. Second, physical factors in CAP cause strong necrosis [36,41,127].…”

Section: Mechanism Discussionsupporting

confidence: 68%

“…As shown in Figure 5 , physically based treatment can affect an area much larger than chemically based treatment because the EM effect can penetrate the dielectric physical barrier between every well on a 96-well plate [ 41 ]. In our recent study, an APPJ’s tip was less than 1 mm; however, the EM effect generated from APPJ could affect an area with a diameter of at least 2 cm [ 91 ]. As shown in Figure 4 a, the abscopal effect affects the tumor at a distance of 2–3 cm.…”

Section: Abscopal Effectmentioning

confidence: 99%

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Preclinical Cold Atmospheric Plasma Cancer Treatment

Limanowski

Yan

Lin

et al. 2022

Cancers

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CAP is an ionized gas generated under atmospheric pressure conditions. Due to its reactive chemical components and near-room temperature nature, CAP has promising applications in diverse branches of medicine, including microorganism sterilization, biofilm inactivation, wound healing, and cancer therapy. Currently, hundreds of in vitro demonstrations of CAP-based cancer treatments have been reported. However, preclinical studies, particularly in vivo studies, are pivotal to achieving a final clinical application. Here, we comprehensively introduced the research status of the preclinical usage of CAP in cancer treatment, by primarily focusing on the in vivo studies over the past decade. We summarized the primary research strategies in preclinical and clinical studies, including transdermal CAP treatment, post-surgical CAP treatment, CAP-activated solutions treatment, and sensitization treatment to drugs. Finally, the underlying mechanism was discussed based on the latest understanding.

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Section: Mechanism Discussionsupporting

confidence: 68%

Section: Abscopal Effectmentioning

confidence: 99%

Preclinical Cold Atmospheric Plasma Cancer Treatment

Limanowski

Yan

Lin

et al. 2022

Cancers

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“…This difference in observed selectivity likely results from the chosen cell comparison. In the past, many studies have compared the sensitivity of cancer cells to CAP with their corresponding healthy cell type [25,33,46]. Although these comparisons provide helpful information, it is important to also consider how CAP influences the immune cell compartment in the tumor microenvironment, which can greatly influence therapy response and patient prognosis [26,27].…”

Section: Discussionmentioning

confidence: 99%

“…Lines, Ma-Mel-19 and UKRV-Mel-15a, in a Dose-Dependent Manner CAP has been previously shown to have potent inhibitory effects on melanoma cells, including the suppression of cell proliferation and the induction of cell death [33,34]. This study expanded on these results by using the human melanoma cell lines, Ma-Mel-19 and UKRV-Mel-15a, and by evaluating their response to direct CAP treatment by the MiniJet-R, an argon and microwave-based plasma jet.…”

Section: Cap Treatment Reduces the Viability And Proliferation Of The...mentioning

confidence: 99%

Oxidative Stress Differentially Influences the Survival and Metabolism of Cells in the Melanoma Microenvironment

Trzeciak

Zimmer

Gehringer

et al. 2022

Cells

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The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T cells, and macrophages, which comprise part of the melanoma microenvironment. To accomplish this, cells were grown in different in vitro cell culture models and were treated with varying amounts of CAP. Subsequent alterations in viability, proliferation, and phenotype were analyzed via flow cytometry and metabolic alterations by Seahorse Cell Mito Stress Tests. It was found that cells generally exhibited reduced viability and proliferation, stemming from CAP induced G2/M cell cycle arrest and subsequent apoptosis, as well as increased mitochondrial stress following CAP treatment. Overall, sensitivity to CAP treatment was found to be cell type dependent with T cells being the most affected. Interestingly, CAP influenced the polarization of M0 macrophages to a “M0/M2-like” phenotype, and M1 macrophages were found to display a heightened sensitivity to CAP induced mitochondrial stress. CAP also inhibited the growth and killed melanoma cells in 2D and 3D in vitro cell culture models in a dose-dependent manner. Improving our understanding of oxidative stress, mechanisms to manipulate it, and its implications for the tumor microenvironment may help in the discovery of new therapeutic targets.

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“…The article by Dayun Yan et al entitled “Anti-Melanoma Capability of Contactless Cold Atmospheric Plasma Treatment” [ 9 ] demonstrates that a cold atmospheric helium plasma jet can prevent proliferation of melanoma cells under contactless treatment conditions. Dayun Yan et al found that the helium gas flow rate was an essential parameter for controlling the electromagnetic effect on melanoma cells.…”

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confidence: 99%