Anti-Melanogenesis, Antioxidant and Anti-Tyrosinase Activities of Scabiosa columbaria L.

Abstract

Scabiosa columbaria is a plant traditionally used to treat skin ailments, such as scabies, wound bruises, sores and hyperpigmentation. To find a novel skin depigmenting agent, the present study was investigated to determine the possible anti-melanogenesis, antioxidant and anti-tyrosinase effects of methanol extract of S. columbaria leaves. Cytotoxicity towards human dermal fibroblast (MRHF) cells was assessed using the live-cell fluorescence imaging microscopy. The inhibitory effects of the extract on tyrosinase, collagenase and melanin synthesis were also investigated using standard in vitro method, while ferric reducing power (FRAP) was used to determine the antioxidant potential of the plant extract. The effect of the extract on collagen content in MRHF cells was also investigated. The plant extract displayed no meaningful cytotoxicity towards MRHF cells and no significant cell death was recorded at all the tested concentrations. The extract (25–100 µg/mL) effectively decreased melanin content in B16F10 (mouse melanoma) cells with moderate inhibition of tyrosinase enzyme in a dose-dependent manner. However, the extract also demonstrated no significant effect on collagenase and collagen content in MRHF cells, but showed strong antioxidant activity at the concentrations tested. The results suggest that S. columbaria could be a promising candidate in the treatment of skin hyperpigmentation disorders

The Feasibility of Utilizing Cultured Cordyceps militaris Residues in Cosmetics: Biological Activity Assessment of Their Crude Extracts

“…Inhibition of the tyrosinase enzyme was investigated via the dopachrome method as previously described [19], with some modifications. L-DOPA was used as a substrate.…”

Chemical compositions by using LC–MS/MS and GC–MS and antioxidant activities of methanolic extracts from leaf and flower parts of Scabiosa columbaria subsp. columbaria var. columbaria L.

“…Chlorogenic and caffeic acids, which are diphenolics have a higher radical scavenging ability than monophenolics (p coumaric acid) ( Rice-Evans et al, 1996 ). Otang-Mbeng and Sagbo (2020) reported that the FRAP test of the leaves of Scabiosa columbaria had high antioxidant activity values due to presence of polyphenolic compounds. Hafsa et al (2009) concluded that extracts (acetonic, ethanolic and aqueous) obtained using different solvents from Scabiosa arenaria Forssk showed high antioxidant activity with different activity tests such as DPPH scavenging capacity (highest inhibition percentages 93.84%), radical cation ABTS .+ scavenging activity (after 5 min from 7.50% to 93.67% for ethanolic extract), TEAC assay (2.25 mM trolox/g extract) and total phenolic content (138.36 mg catechin/ g extract).…”

“…Although researches have been carried out to determine the chemical composition and biological activities of Scabiosa columbaria L. at the species and subspecies category ( Horn et al, 2001 , Benli et al, 2008 , Van Vuuren and Naidoo, 2010 , Otang-Mbeng and Sagbo, 2020 , Sagbo et al, 2020 ), no research has yet been carried out to determine the characteristics specified at the variety category.…”

Cytotoxic, Cellular Antioxidant, and Antiglucuronidase Properties of the Ethanol Leaf Extract from Bulbine asphodeloides

“…The cytotoxic activity of B. asphodeloides on MRHF cells was assessed by the Hoechst 33342/PI dual-staining method [ 17 ]. Cells were seeded in a 96 well-plate at a density of 8000 cells/well using aliquots (100 μ L) and left to attach overnight.…”