Anti-lymphangiogenic diterpenes from the bark of Calocedrus macrolepis var. formosana

Lee, Tzong‐Huei; Hsieh, Chin‐Lin; Wu, Ho-Cheng; Wang, Shih‐Wei; Yu, Chen-Lin; Hsiao, George; Cheng, Ming‐Jen; Hsieh, Wen‐Tsong; Kuo, Yueh‐Hsiung

doi:10.38212/2224-6614.3375

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…The protocols for cell culture of human endothelial progenitor cells (EPCs) have been described in detail previously [ 11 ]. The anti-angiogenic activities of selected compounds were evaluated by cell growth assay according to our previous method [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Chemical constituents from a marine medicinal brown alga-derived Xylaria acuta SC1019

Hsi,

Wang,

Hsiao

et al. 2024

Journal of Food and Drug Analysis

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Section: Methodsmentioning

confidence: 99%

Chemical constituents from a marine medicinal brown alga-derived Xylaria acuta SC1019

Hsi,

Wang,

Hsiao

et al. 2024

Journal of Food and Drug Analysis

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“…formosana have demonstrated that the leaf, bark, heartwood, and seed essential oils exhibit various beneficial properties. [3][4][5][6][7][8][9][10] These include antioxidant effects, antifungal properties, antibacterial activity, antitermitic properties, mosquito-repellent properties, anti-inflammatory effects, inhibition of lymphangiogenesis, and potential anticancer activity. Based on the available information, there have been no studies conducted to examine the chemical composition or biological activity of the essential oils derived from the twigs of C macrolepis var.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and In Vitro Antimicrobial, Antioxidant, and Tyrosinase-Inhibiting Activity of Essential Oils Extracted From the Leaves and Twigs of Calocedrus macrolepis var. formosana

Shyu,

Hsu,

Yang

et al. 2023

Natural Product Communications

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In this study, we focused on evaluating the antimicrobial, antioxidant, and tyrosinase-inhibiting properties of leaf and twig essential oils from Calocedrus macrolepis var. formosana. Additionally, we determined the major components associated with these effects. The findings revealed that α-pinene (36.4%) and limonene (23.6%) were the dominant components in the leaf essential oil, while α-pinene (36.2%), ferruginol (22.5%), and limonene (13.1%) were the major constituents in the twig essential oil. However, when comparing the biological activities of the 2 essential oils, the twig essential oil demonstrated excellent inhibitory effects against skin pathogens, as well as strong antioxidant and tyrosinase-inhibiting activities, in contrast to the leaf essential oil. The twig essential oil also exhibited excellent antimicrobial activity against 5 skin pathogenic strains, with the strongest inhibitory effect being that against Staphylococcus aureus, S epidermidis, and Candida albicans, which was accompanied by an inhibition zone of 46 to 48 mm, a minimum inhibitory concentration (MIC) of 125 μg/mL, and a minimum bactericidal concentration (MBC) of 250 μg/mL. The inhibitory activity of the twig essential oil against lipid peroxidation was found to be potent, as evidenced by an IC50 value of 216 ± 1.9 μg/mL. It also exhibited strong scavenging activity against free radicals, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 121.6 ± 0.5 μg/mL) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (IC50 = 136.8 ± 1.6 μg/mL) cation radicals. In addition, it exhibited a high cupric ion reducing–antioxidant capacity (CUPRAC), with the A0.50 value determined to be 186.28 ± 0.5 μg/mL. It also exhibited adequate activity in terms of inhibiting in vitro tyrosinase activity, as evidenced by an IC50 value of 168.5 ± 0.6 μg/mL. Further analysis revealed that ferruginol was the active component responsible for the antimicrobial, antioxidative, and tyrosinase-inhibiting activities observed in twig oil. These findings suggest that the twig oil is a promising candidate for further research because of its favorable antimicrobial, antioxidative, and tyrosinase-inhibiting activity.

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“…Terpenoids are renowned for their cytotoxic effects on various types of tumors. Interestingly, in the previous investigation, it was discovered that diterpenes possess anti-lymphangiogenic properties, which is a new concept in targeting tumor cell metastasis [37][38][39]. To further explore the relationship between terpenoids and their anti-lymphangiogenic activity, as well as to expand the pharmacological profile of terpenoids, we evaluated the anti-lymphangiogenic activity of the isolated compounds in our study.…”

Section: Introductionmentioning

confidence: 99%

Anti-Lymphangiogenic Terpenoids from the Heartwood of Taiwan Juniper, Juniperus chinensis var. tsukusiensis

Wu,

Shiu,

Wang

et al. 2023

Plants

Self Cite

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To look in-depth into the phytochemical and pharmacological properties of Taiwan juniper, this study investigated the chemical profiles and anti-lymphangiogenic activity of Juniperus chinensis var. tsukusiensis. In this study, four new sesquiterpenes, 12-acetoxywiddrol (1), cedrol-13-al (2), α-corocalen-15-oic acid (3), 1,3,5-bisaoltrien-10-hydroperoxy-11-ol (4), one new diterpene, 1β,2β-epoxy-9α-hydroxy-8(14),11-totaradiene-3,13-dione (5), and thirty-three known terpenoids were successfully isolated from the heartwood of J. chinensis var. tsukusiensis. The structures of all isolates were determined through the analysis of physical data (including appearance, UV, IR, and optical rotation) and spectroscopic data (including 1D, 2D NMR, and HRESIMS). Thirty-four compounds were evaluated for their anti-lymphangiogenic effects in human lymphatic endothelial cells (LECs). Among them, totarolone (6) displayed the most potent anti-lymphangiogenic activity by suppressing cell growth (IC50 = 6 ± 1 µM) of LECs. Moreover, 3β-hydroxytotarol (7), 7-oxototarol (8), and 1-oxo-3β-hydroxytotarol (9) showed moderate growth-inhibitory effects on LECs with IC50 values of 29 ± 1, 28 ± 1, and 45 ± 2 µM, respectively. Totarolone (6) also induced a significant concentration-dependent inhibition of LEC tube formation (IC50 = 9.3 ± 2.5 µM) without cytotoxicity. The structure–activity relationship discussion of aromatic totarane-type diterpenes against lymphangiogenesis of LECs is also included in this study. Altogether, our findings unveiled the promising potential of J. chinensis var. tsukusiensis in developing therapeutics targeting tumor lymphangiogenesis.

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Anti-lymphangiogenic diterpenes from the bark of Calocedrus macrolepis var. formosana

Cited by 5 publications

References 29 publications

Chemical constituents from a marine medicinal brown alga-derived Xylaria acuta SC1019

Chemical constituents from a marine medicinal brown alga-derived Xylaria acuta SC1019

Chemical Composition and In Vitro Antimicrobial, Antioxidant, and Tyrosinase-Inhibiting Activity of Essential Oils Extracted From the Leaves and Twigs of Calocedrus macrolepis var. formosana

Anti-Lymphangiogenic Terpenoids from the Heartwood of Taiwan Juniper, Juniperus chinensis var. tsukusiensis

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