Anti-lipoteichoic acid antibodies enhance release of cytokines by monocytes sensitized with lipoteichoic acid

Mancuso, Giuseppe; Tomasello, Francesco; Ofek, Itzhak; Teti, Giuseppe

doi:10.1128/iai.62.4.1470-1473.1994

Cited by 32 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether alanine substituents play a decisive role in the immunobiology of LTA from other bacterial species deserves further study. It might be speculated that alanine substituents enable hydrophobic interactions between LTA molecules; dimerization of LTA molecules by antiglycerophosphate antibodies has been shown to amplify cytokine induction by LTA (20).…”

mentioning

confidence: 99%

Structure–Function Relationship of Cytokine Induction by Lipoteichoic Acid fromStaphylococcus aureus

Morath

Geyer

Hartung

2001

The Journal of Experimental Medicine

426

463

View full text Add to dashboard Cite

Lipoteichoic acids (LTAs) have been proposed as putative Gram-positive immunostimulatory counterparts to Gram-negative lipopolysaccharides. However, LTA from Staphylococcus aureus, the clinically most frequent Gram-positive pathogen, was inactive after purification. Here, a novel isolation procedure to prepare pure (>99%) biologically active LTA, allowing the first structural analysis by nuclear magnetic resonance and mass spectrometry, is described. A comparison with LTA purified by standard techniques revealed that alanine substituents are lost during standard purification, resulting in attenuated cytokine induction activity. In line with this finding, hydrolysis of alanine substituents of active LTA decimated cytokine induction. LTA represents a major immunostimulatory component of S. aureus.

show abstract

mentioning

confidence: 99%

Structure–Function Relationship of Cytokine Induction by Lipoteichoic Acid fromStaphylococcus aureus

Morath

Geyer

Hartung

2001

The Journal of Experimental Medicine

426

463

View full text Add to dashboard Cite

show abstract

“…HlpA-LTA complexes accumulating on host cell surfaces or forming in situ might serve to bridge several membrane receptors, resulting in a synergistic signal for the induction of cytokine synthesis and secretion. Indeed, cross-linking of LTA on the surfaces of monocytes by F(ab) 2 fragments of immunoglobulin G has been reported to aggregate receptors and enhance cytokine production (28). It was also reported that once the LTA receptors on the monocytes were fully aggregated, the quantities of cytokines produced were similar to those produced by monocytes induced by LPS.…”

Section: Discussionmentioning

confidence: 96%

“…The inflammation of renal tissue, observed in SAN, is generally believed to result from the action of anaphylatoxins generated by the classical complement pathway (12,27,29,38); however, certain streptococcal components, including LTA, can directly induce monocytes (2,5,24,28,31) and endothelial cells (37) to synthesize and secrete the proinflammatory cytokines tumor necrosis factor alpha (TNF-␣), interleukin-1 (IL-1), and IL-6. These cytokines activate T and B lymphocytes, stimulate fibroblast proliferation, and induce local vascular endothelial cells to synthesize adhesion receptors that mediate extravasation of leukocytes (21,36).…”

mentioning

confidence: 99%

Streptococcal Histone Induces Murine Macrophages To Produce Interleukin-1 and Tumor Necrosis Factor Alpha

Zhang¹,

Ignatowski

Spengler

et al. 1999

Infect Immun

View full text Add to dashboard Cite

The histone-like protein (HlpA) is highly conserved among streptococci. After lysis of streptococci in infected tissues, HlpA can enter the bloodstream and bind to proteoglycans in the glomerular capillaries of kidneys, where it can react with antibodies or stimulate host cell receptors. Deposits of streptococcal antigens in tissues have been associated with localized acute inflammation. In this study, we measured the ability of purified HlpA (5 to 100 g/ml), from Streptococcus mitis, to induce the production of proinflammatory cytokines by cultured, murine peritoneal macrophages. The release of tumor necrosis factor alpha (TNF-␣) and interleukin-1 (IL-1) was time and concentration dependent and was not diminished by the presence of polymyxin B. Exposure of macrophages to a mixture of HlpA and lipoteichoic acid resulted in a synergistic response in the production of both TNF-␣ and IL-1. Stimulation with a mixture of HlpA and heparin resulted in reduced cytokine production (50% less IL-1 and 76% less TNF-␣) compared to that by cells incubated with HlpA alone. The inclusion of antibodies specific to HlpA in macrophage cultures during stimulation with HlpA did not affect the quantity of TNF-␣ or IL-1 produced. These observations suggest that streptococcal histone may contribute to tissue injury at infection sites by promoting monocytes/macrophages to synthesize and release cytokines that initiate and exacerbate inflammation. Streptococcus pyogenes, which can infect tissues in enormous numbers, may release sufficient amounts of HlpA to reach the kidneys and cause acute poststreptococcal glomerulonephritis.

show abstract

“…Serum specimens of the donors of whole blood cultures in the present and previous studies contained considerable levels of anti-LTA antibodies (Arakaki, R., Sato, M. and Takada, H., unpublished) which might in£uence the bioactivities of LTA preparations. Mancuso et al [18] reported that anti-LTA antibodies enhanced the release of cytokines by human monocytes sensitized with LTA. It is well known that serum factors such as LBP [19^21], septin [22] and sCD14 [23,24] are required for e¤cient cellular activation.…”

Section: Discussionmentioning

confidence: 99%

A lipoteichoic acid fraction of Enterococcus hirae activates cultured human monocytic cells via a CD14-independent pathway to promote cytokine production, and the activity is inhibited by serum components

Arakaki¹

1998

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

To elucidate the cellular activation mechanisms of lipoteichoic acid (LTA) compared with those of lipopolysaccharide (LPS), a quantitatively major LTA fraction, QM-1M, was prepared from hot phenol-water extracts of Enterococcus hirae (ATCC 9790) by hydrophobic octyl-Sepharose chromatography and by ion-exchange membrane (QMA-Mem Sep 1010) chromatography as a 60% 1-propanol- and 1 M NaCl-eluted fraction. Unlike the reference Escherichia coli LPS, QM-1M did not demonstrate any ability to induce cytokines in a human whole blood culture system in this study, whereas QM-1M induced a few cytokines such as interleukin (IL)-8 and tumor necrosis factor-alpha in human monocytic THP-1 cell and human peripheral mononuclear cell (PBMC) cultures in the absence of serum. Fetal calf and human sera decreased the above cytokine induction by QM-1M in THP-1 and PBMC cultures, whereas sera increased activities of the reference LPS. IL-8 induction in the absence of serum in response to QM-1M was demonstrated to proceed through a CD14-independent pathway unlike the reference LPS.

show abstract

Anti-lipoteichoic acid antibodies enhance release of cytokines by monocytes sensitized with lipoteichoic acid

Cited by 32 publications

References 24 publications

Structure–Function Relationship of Cytokine Induction by Lipoteichoic Acid fromStaphylococcus aureus

Structure–Function Relationship of Cytokine Induction by Lipoteichoic Acid fromStaphylococcus aureus

Streptococcal Histone Induces Murine Macrophages To Produce Interleukin-1 and Tumor Necrosis Factor Alpha

A lipoteichoic acid fraction of Enterococcus hirae activates cultured human monocytic cells via a CD14-independent pathway to promote cytokine production, and the activity is inhibited by serum components

Contact Info

Product

Resources

About