Anti-leukaemic and Anti-mutagenic Effects of Di(2-ethylhexyl)phthalate Isolated from Aloe vera Linne

Abstract

Extracts of Aloe vera Linne have been found to exhibit cytotoxicity against human tumour cell lines. This study examines the anti-tumour effects of di(2-ethylhexyl)phthalate (DEHP) isolated from Aloe vera Linne, in human and animal cell lines. Its anti-mutagenic effects were examined using Salmonella typhimurium TA98 and TA100 strains. Growth inhibition was specifically exerted by DEHP against three leukaemic cell lines at concentrations below 100 microg mL(-1). At 100 microg mL(-1) DEHP, K562, HL60 and U937 leukaemic cell lines showed growth inhibition of 95, 97 and 95%, respectively. DEHP exhibited an inhibitory activity of 74, 83 and 81%, respectively, in K562, HL60 and U937 cell lines at a concentration of 10 microg mL(-1). At a concentration of 1 microg mL(-1), DEHP exerted an inhibitory activity of 50, 51 and 52%, respectively, in K562, HL60 and U937. In a normal cell line, MDBK, DEHP exerted 30% growth inhibition at a concentration of 100 microg mL(-1), and showed no inhibitory activity at concentrations below 50 microg mL(-1). It was found that DEHP exerted anti-mutagenic activity in the Salmonella mutation assay. The number of mutant colonies of Salmonella typhimurium strain TA98 upon exposure to AF-2 (0.2 microg/plate) decreased in a concentration-dependent manner in the presence of different DEHP concentrations (decreasing to 90.4, 83.9, 75.4, 69.6 and 46.9%, respectively, for DEHP concentrations of 100, 50, 10, 5 and 1 microg/plate). In the case of Salmonella typhimurium strain TA100, DEHP reduced AF-2-induced mutagenicity at 1, 5, 10, 50 and 100 microg/plate to 57.4, 77.5, 80.0, 89.0 and 91.5%, respectively. The isolated compound from Aloe vera Linne, DEHP, was considered to be the active principle responsible for anti-leukaemic and anti-mutagenic effects in-vitro.

Isolation and Identification of an Antimutagenic Phthalate Derivative Compound from Octopus (<i>Paraoctopus limaculatus</i>)

“…Lee et al [20] isolated di(2-ethylhexyl) phthalate from Aloe vera Linnen. They found this phthalate derivative to have anti-leukemic and antimutagenic properties as assayed in human tumor cell lines and Salmonella typhimurium TA98 and TA100, respectively.…”

Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain

“…Phthalate compounds are petrochemicals used as plasticisers or solvents in a variety of industrial products. Nevertheless, many phthalate derivatives have been isolated from terrestrial and marine organisms including plants (Lee et al 2000), fungal and bacterial culture broths, especially those belonging to the genus Streptomyces. Compound 2 has been already described from Streptomyces bangladeshiensis (Al- Bari et al 2006).…”

Isolation and Characterization of a PsychrotolerantStreptomycesStrain from Permafrost Soil in Spitsbergen, Producing Phthalic Acid Ester

“…the biosynthesis of DehP by red alga Bangia atropurpurea was described from chen (4). DehP isolated from Aloe vera linne shows anti-leukaemic and anti-mutagenic effects (15).…”