Anti-interferon activity of adenovirus-2-encoded VAI and VAII RNAs in translation in cultured human cells

Mori, Kensaku; Jüttermann, Ruth; Wienhues, Ulla; Kobayashi, K; Yagi, Masahide; Sugimoto, Toshiyuki; Tjia, Sian T.; Doerfler, Walter; Hosokawa, Keiichi

doi:10.1016/0168-1702(95)01309-1

Cited by 10 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VA RNA I is an adenoviral RNA pol‐III transcript abundantly expressed late during infection to prevent PKR activation by viral dsRNA. It contains an apical dsRNA stem, which binds to the PKR dsRNA binding site, and a central domain, which inhibits the kinase (Mathews and Shenk, 1991; Ma and Mathews, 1993; Mori et al, 1996). VA RNA I contains all the major transcription regulatory elements within the transcribed region.…”

Section: Resultsmentioning

confidence: 99%

Determinants of interferon-stimulated gene induction by RNAi vectors

2004

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Determinants of interferon-stimulated gene induction by RNAi vectors

2004

View full text Add to dashboard Cite

“…The encoded partially double-stranded RNAs are approximately 160 nucleotides in size, and are involved in translational control and inhibition of the interferon response (Mathews & Shenk, 1991;Mori et al, 1996). A 90 nucleotide pol III RNA is also produced by a leftward-oriented gene located between ORF16 and ORF9 in FAdV-1 (Larsson et al, 1986).…”

Section: Genus-specific Genesmentioning

confidence: 99%

Genetic content and evolution of adenoviruses

Davison¹,

Benkő

Harrach

2003

Journal of General Virology

535

458

View full text Add to dashboard Cite

This review provides an update of the genetic content, phylogeny and evolution of the family Adenoviridae. An appraisal of the condition of adenovirus genomics highlights the need to ensure that public sequence information is interpreted accurately. To this end, all complete genome sequences available have been reannotated. Adenoviruses fall into four recognized genera, plus possibly a fifth, which have apparently evolved with their vertebrate hosts, but have also engaged in a number of interspecies transmission events. Genes inherited by all modern adenoviruses from their common ancestor are located centrally in the genome and are involved in replication and packaging of viral DNA and formation and structure of the virion. Additional niche-specific genes have accumulated in each lineage, mostly near the genome termini. Capture and duplication of genes in the setting of a 'leader-exon structure', which results from widespread use of splicing, appear to have been central to adenovirus evolution. The antiquity of the pre-vertebrate lineages that ultimately gave rise to the Adenoviridae is illustrated by morphological similarities between adenoviruses and bacteriophages, and by use of a protein-primed DNA replication strategy by adenoviruses, certain bacteria and bacteriophages, and linear plasmids of fungi and plants. INTRODUCTIONThe purpose of this review is to provide a comprehensive update of the genetic content, phylogeny and evolution of the family Adenoviridae, whose members infect hosts throughout the vertebrates (Russell & Benkó´, 1999). This area has frequently taken a back seat to studies of the interactions of selected human adenovirus proteins with cellular processes and, more recently, to the use of adenoviruses as vectors. Our aim is to redress this imbalance by bringing together published information and by offering fresh insights into the genomics of the family as a whole. It is not our purpose to deal in broad scope with other areas, such as the expression, functions and interactions of adenovirus gene products and the ways in which host defences are manipulated during the infectious cycle. These have been reviewed recently by Russell (2000) and Shenk (2001) for human adenoviruses. Phylogeny, classification and genetic organizationMembers of the family Adenoviridae are non-enveloped, icosahedral viruses that replicate in the nucleus. Their linear, double-stranded DNA molecules are 26-45 kbp in size and rank as medium-sized among the DNA viruses. The genomes are characterized by an inverted terminal repeat (ITR) ranging in size from 36 to over 200 bp, and the 59 ends are linked to a terminal protein (TP). Phylogenetic relationships among a large number of adenoviruses infecting vertebrates from fish to humans are shown in Fig. 1. The major clades (groups of viruses sharing a common ancestor) correspond to the four accepted genera plus a fifth genus that is likely to be added in due course (Benkó´et al., 2002;. Two genera (Mastadenovirus and Aviadenovirus) originate from mammals or birds, respective...

show abstract

“…Its limited ability to block PKR activation (10,11) argues against a role similar to that of VA RNA I , especially in light of its lesser accumulation in infected cells (12). Furthermore, a mutant virus that fails to produce VA RNA II grows as well as wild type in culture (13).…”

mentioning

confidence: 99%

Activities of adenovirus virus-associated RNAs: Purification and characterization of RNA binding proteins

Liao

Kobayashi

Mathews

1998

Proc. Natl. Acad. Sci. U.S.A.

122

View full text Add to dashboard Cite

Most human adenoviruses encode two virusassociated (VA) RNAs, VA RNA I and VA RNA II , that accumulate to high levels in the cytoplasm of infected cells. The function of VA RNA I in blocking the activation of the cellular kinase PKR is well known, but the role of VA RNA II is obscure. Herein we characterize and purify several human proteins that interact preferentially with VA RNA II in Northwestern blot assays. Two of these proteins were identified as RNA helicase A and NF90, a component of the heterodimeric nuclear factor of activated T cells (NFAT). They copurified with the smaller NFAT subunit, NF45, which did not bind VA RNA II , and with an unidentified protein, p97, which did bind VA RNA II . Both RNA helicase A and NF90 contain two copies of a double-stranded (ds) RNA binding motif and bind strongly to dsRNA. NF90 interacts with RNAs in the following order of affinity: dsRNA > VA RNA II > VA RNA I > singlestranded RNA. Furthermore, VA RNA II is more effective than VA RNA I as an inhibitor of RNA helicase activity. These data identify RNA helicase A and NF90 as cellular proteins with an affinity for dsRNA and other structured RNA molecules and suggest that their functions are subject to regulation by RNA ligands including VA RNA II .

show abstract

Anti-interferon activity of adenovirus-2-encoded VAI and VAII RNAs in translation in cultured human cells

Cited by 10 publications

References 30 publications

Determinants of interferon-stimulated gene induction by RNAi vectors

Determinants of interferon-stimulated gene induction by RNAi vectors

Genetic content and evolution of adenoviruses

Activities of adenovirus virus-associated RNAs: Purification and characterization of RNA binding proteins

Contact Info

Product

Resources

About