Anti-influenza A virus effect of Hypericum perforatum L. extract

Pu, Ximing; Liang, Jianping; Wang, Xuehong; Xu, Tao; Liu, Hua; Shang, Ruofeng; Yu, Li; Xing, Yan-mei

doi:10.1007/s12250-009-2983-x

Cited by 18 publications

(13 citation statements)

References 28 publications

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“…Hypericin is an anthraquinone predominantly an active component of Hypericum perforatum, a flowering plant belonging to Hypericaceae family was effective against viral protease with a binding energy of À11.17 kcal/mol. The plant is well known for its activity against several viruses namely, influenza A, herpes simplex virus, bronchitis virus, and human immune viruses (Chen et al, 2019;Pu et al, 2009 (Islam et al, 2020) similar to the present study. Robustaflavone, a natural flavonoid formed by combining two molecules of apigenin through oxidative coupling forming hydrogen bond between C3 and C6 carbon atoms of hydroxyphenyl and chromene ring respectively (Lin et al, 2000;Xu et al, 2009;Zengion & Yarnell, 2011).…”

Section: Resultssupporting

confidence: 84%

Molecular docking, validation, dynamics simulations, and pharmacokinetic prediction of natural compounds against the SARS-CoV-2 main-protease

Shivanika

Kumar²,

Ragunathan

et al. 2020

Journal of Biomolecular Structure and Dynamics

145

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The study aims to evaluate the potency of two hundred natural antiviral phytocompounds against the active site of the Severe Acquired Respiratory Syndrome-Coronavirus À 2 (SARS-CoV-2) Main-Protease (M pro) using AutoDock 4.2.6. The three-dimensional crystal structure of the M pro (PDB Id: 6LU7) was retrieved from the Protein Data Bank (PDB), the active site was predicted using MetaPocket 2.0. Food and Drug Administration (FDA) approved viral protease inhibitors were used as standards for comparison of results. The compounds theaflavin-3-3'-digallate, rutin, hypericin, robustaflavone, and (-)-solenolide A with respective binding energy of À12.41 (Ki ¼ 794.96 pM); À11.33 (Ki ¼ 4.98 nM); À11.17 (Ki ¼ 6.54 nM); À10.92 (Ki ¼ 9.85 nM); and À10.82 kcal/mol (Ki ¼ 11.88 nM) were ranked top as Coronavirus Disease À 2019 (COVID-19) M pro inhibitors. The interacting amino acid residues were visualized using Discovery Studio 3.5 to elucidate the 2-dimensional and 3-dimensional interactions. The study was validated by i) re-docking the N3-peptide inhibitor-M pro and superimposing them onto co-crystallized complex and ii) docking decoy ligands to M pro. The ligands that showed low binding energy were further predicted for and pharmacokinetic properties and Lipinski's rule of 5 and the results are tabulated and discussed. Molecular dynamics simulations were performed for 50 ns for those compounds using the Desmond package, Schr€ odinger to assess the conformational stability and fluctuations of protein-ligand complexes during the simulation. Thus, the natural compounds could act as a lead for the COVID-19 regimen after in-vitro and in-vivo clinical trials.

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Section: Resultssupporting

confidence: 84%

Molecular docking, validation, dynamics simulations, and pharmacokinetic prediction of natural compounds against the SARS-CoV-2 main-protease

Shivanika

Kumar²,

Ragunathan

et al. 2020

Journal of Biomolecular Structure and Dynamics

145

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“… Citronellol and Eugenol (Leaves, Essential oil) 1,8-Cineole and α-Thujone (Leaves) Inhibits the hemagglutinin activity, but not the Neuraminidase activity Vimalanathan and Hudson (2014) Glycyrrhiza glabra L. 3,4-dihydro-8,8-dimethyl-2H,8H-benzo dipyran-3-ol, Biochanin B, Glabrol, Glabrone, Hispaglabridin B, Licoflavone B, Licorice glycoside B, Licorice glycoside E, Liquiritigenin, Liquiritin, Prunin (Roots) Inhibit Neuraminidase (NA) activity Grienke et al (2014) Hypericum perforatum L. Hypericin (Flowers) Inhibits virus-induced cytopathic effect; ie: Lung consolidation and lessening of lung virus titers. Pu et al (2009) Hypericum perforatum L. Isoquercetin (Flowers) Inhibit the replication of both influenza A and B viruses at the lowest effective concentration Kim et al (2010) Hypericum perforatum L. Chlorogenic acid and Quercetin (Flowers) Taken together, it was proposed that chlorogenic acid and quercetin could be employed as the effective lead compounds for anti-influenza A H1N1 due to having strong binding abilities with neuraminidase. Liu et al (2016) Malus domestica Borkh.…”

Section: Resultsmentioning

confidence: 99%

Potential anti-influenza effective plants used in Turkish folk medicine: A review

Sargın

2021

Journal of Ethnopharmacology

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Ethnopharmacological relevance Due to the outbreaks such as SARS, bird flu and swine flu, which we frequently encounter in our century, we need fast solutions with no side effects today more than ever. Due to having vast ethnomedical experience and the richest flora (34% endemic) of Europe and the Middle East, Turkey has a high potential for research on this topic. Plants that locals have been using for centuries for the prevention and treatment of influenza can offer effective alternatives to combat this problem. In this context, 224 herbal taxa belonging to 45 families were identified among the selected 81 studies conducted in the seven regions of Turkey. However, only 35 (15.6%) of them were found to be subjected to worldwide in vitro and in vivo research conducted on anti-influenza activity. Quercetin and chlorogenic acid, the effectiveness of which has been proven many times in this context, have been recorded as the most common (7.1%) active ingredients among the other 56 active substances identified. Aim of the study This study has been carried out to reveal the inventory of plant species that have been used in flu treatment for centuries in Turkish folk medicine, which could be used in the treatment of flu or flu-like pandemics, such as COVID 19, that humanity has been suffering with, and also compare them with experimental studies in the literature. Materials and methods The investigation was conducted in two stages on the subject above by using electronic databases, such as Web of Science, Scopus, ScienceDirect, ProQuest, Medline, Cochrane Library, EBSCO, HighWire Press, PubMed and Google Scholar. The results of both scans are presented in separate tables, together with their regional comparative analysis. Results Data obtained on taxa are presented in a table, including anti-influenza mechanism of actions and the active substances. Rosa canina (58.7%) and Mentha x piperita (22.2%) were identified as the most common plants used in Turkey. Also, Sambucus nigra (11.6%) , Olea europaea (9.3%), Eucalyptus spp., Melissa officinalis, and Origanum vulgare (7.0%) emerged as the most investigated taxa. Conclusion This is the first nationwide ethnomedical screening work conducted on flu treatment with plants in Turkey. Thirty-nine plants have been confirmed in the recent experimental anti-influenza research, which strongly shows that these plants are a rich pharmacological source. Also, with 189 (84.4%) taxa, detections that have not been investigated yet, they are an essential resource for both national and international pharmacological researchers in terms of new natural medicine searches. Considering that the production of antimalarial drugs...

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“…Because this experiment was carried out under the condition of no oxygen and weak light, the direct attack effect of HY on virus particles was not brought into full play, and the killing effect of virus was weakened (Miskovsky, 2002;Xu et al, 2019). In the pre-treatment of HY on cells prior to infection, HY blockaded on the adsorption of virus in CEK cells was weak, or did not protect the cells, thus failed to effectively prevent IBV from entering the host cells (Pu et al, 2009(Pu et al, , 2012. Therefore, in the following experiments, the CEK cells were first infected IBV, and then incubated HY solution.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells

et al. 2019

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Avian infectious bronchitis virus (IBV), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. Hypericin (HY) is an excellent compound that has been investigated in antiviral, antineoplastic, and antidepressant. To investigate the inhibition effect of HY on IBV infection in chicken embryo kidney (CEK) cells, 3 different experimental designs: pre-treatment of cells prior to IBV infection, direct treatment of IBV-infected cells, and pre-treatment of IBV prior to cell infection were used. Quantitative real-time PCR (qRT-PCR), immunofluorescence assay (IFA), flow cytometry, and fluorescence microscopy were performed and virus titer was determined by TCID 50 . The re-sults revealed that HY had a good anti-IBV effect when HY directly treated the IBV-infected cells, and virus infectivity decreased in a dose-dependent manner. Furthermore, HY inhibited IBV-induced apoptosis in CEK cells, and significantly reduced the mRNA expression levels of Fas, FasL, JNK, Bax, Caspase 3, and Caspase 8, and significantly increased Bcl-2 mRNA expression level in CEK cells. In addition, HY treatment could decrease IBV-induced reactive oxygen species (ROS) generation in CEK cells. These results suggested that HY showed potential antiviral activities against IBV infection involving the inhibition of apoptosis and ROS generation in CEK cells.

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Anti-influenza A virus effect of Hypericum perforatum L. extract

Cited by 18 publications

References 28 publications

Molecular docking, validation, dynamics simulations, and pharmacokinetic prediction of natural compounds against the SARS-CoV-2 main-protease

Molecular docking, validation, dynamics simulations, and pharmacokinetic prediction of natural compounds against the SARS-CoV-2 main-protease

Potential anti-influenza effective plants used in Turkish folk medicine: A review

Protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells

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