Anti-inflammatory Terpenes from Flowers of Inula japonica

Abstract

Five new terpenes (1-5) and ten known compounds (6-15) were isolated from Inula japonica, and their structures were identified by spectroscopic analysis. Compounds 3 and 14 showed positive inhibitory effects on nitric oxide production. Furthermore, compound 14 suppressed both leukotriene C4 synthesis and degranulation in c-kit ligand-induced bone marrow-derived mast cells.

DNA Topoisomerase Inhibitory Activity of Constituents from the Flowers of <i>Inula japonica</i>

“…Two new (1, 2) and twelve known compounds (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) were isolated by various chromatographic separations from the EtOAc extract of the flowers of I. japonica (Fig. 1) (Fig.…”

“…Structures of 3-14 were identified by comparison of 1 H-and C 13 -NMR, FAB-MS spectral data and optical rotation values with those previously reported to be budlein B (3), 23) 6β-hydroxytomentosin (4), 24) 6-deacetoxybritanin (5), 25) 4-epipulchellin (6), 26) britanin (7), 27) tomentosin (8), 28) (+)-dihydroquercetin (9), 29) (−)-syringaresinol (10), 30) quercetagetin 3,4′-dimethy ether (11), 31) luteolin (12), 32) britanin G (13) 33) and inuchinenolide C (14) 34) (see the Supplementary materials). These compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) were tested for inhibitory activities of DNA topoisomerases I and II (Fig. 5, Table 1).…”

“…1) Some terpenes [2][3][4][5][6][7] and flavonoids 8,9) have previously been isolated from this medicinal plant and have been reported to possess diverse biological activities, such as anti-diabetes, 10) antihypolipidemia, 11) hepatoprotective, 12) anti-inflammatory 7) and anti-tumor 1,2,13) effects. We have been isolating compounds that inhibited topoisomerases I and II from the source of medicinal plants.…”

2α-Hydroxyeudesma-4,11(13)-Dien-8β,12-Olide Isolated from Inula britannica Induces Apoptosis in Diffuse Large B-cell Lymphoma Cells

“…Such compounds exhibit a variety of pharmacological activities, including cytotoxic and antitumor activities, and consequently, plants containing these compounds are promising ingredients for novel anticancer candidate drugs of natural origin [30]. Inula britannica is an erect, sunflower-like, herbaceous biennial or perennial plant in the Asteraceae family and its flowers are used in traditional Chinese medicine for the treatment of various diseases, such as digestive disorders, bronchitis, and inflammation [31]. This plant has been shown to contain high levels of sesquiterpenes, and some of these sesquiterpenes, especially sesquiterpene lactones, have been reported to exhibit potent cytotoxic and apoptotic activities [14,15].…”

IVSE, isolated from Inula japonica,suppresses LPS-induced NO production via NF-κB and MAPK inactivation in RAW264.7 cells

“…In our previous studies, we demonstrated that flower extract of I. japonica showed anti-inflammatory and anti-asthmatic activities in vivo and in vitro [4,20,26]. We also isolated several compounds from I. japonica and determined their anti-inflammatory activities [33]. Britanin, another compound isolated from the flowers of I. japonica, inhibited the production of NO and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 cells [25].…”