Anti-inflammatory properties ofLactobacillus gasseriexpressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis

Abstract

Emerging evidence has implicated reactive oxygen species (ROS) in the pathogenesis of inflammatory bowel disease (IBD). Although intestinal epithelial cells produce the ROS-neutralizing enzyme superoxide dismutase (SOD), the protein and activity levels of copper/zinc (Cu/Zn) and manganese (Mn) SOD are perturbed in inflamed tissues of IBD patients. Thus we investigated the ability of MnSOD from Streptococcus thermophilus to reduce colitis symptoms in interleukin (IL) 10-deficient mice using Lactobacillus gasseri as a delivery vehicle. Cohorts of 13-15 IL-10-deficient mice were left untreated or supplemented with native L. gasseri or L. gasseri expressing MnSOD for 4 wk. Colonic tissue was collected and inflammation was histologically scored. The presence of innate immune cells was investigated by immunohistochemistry and the host antioxidant response was determined by quantitative PCR. It was demonstrated that L. gasseri was stably maintained in mice for at least 3 days. L. gasseri producing MnSOD significantly reduced inflammation in IL-10-deficient mice compared with untreated controls (P < 0.05), whereas the anti-inflammatory effects of both native and MnSOD producing L. gasseri were more pronounced in males. The anti-inflammatory effects of L. gasseri were associated with a reduction in the infiltration of neutrophils and macrophages. Transcripts of antioxidant genes were equivalent in colonic tissues obtained from control and probiotic-treated IL-10-deficient mice. This study demonstrates that L. gasseri producing MnSOD has significant anti-inflammatory activity that reduces the severity of colitis in the IL-10-deficient mouse.

Gut microbiome and anticancer immune response: really hot Sh*t!

“…91 L. gasseri genetically modified to overexpress superoxide dismutase decreased colitis in IL-10-deficient hosts. 92 Prebiotics refer to indigestible food ingredients that selectively promote the colonization of healthy commensals such as the dietary fiber inulin that promotes Bifidobacteria growth. More specifically, cancer-preventive antioxidants include dietary polyphenols (flavonoids, phenolic acids, lignins present in tea, wine, nuts, fruits, and so on, and ellagic acid metabolized by colonic microbiota into urolithins exhibiting antiestrogenic and anti-COX2 activities).…”

Protective effect of myricetin in dextran sulphate sodium-induced murine ulcerative colitis

“…Although their precise aetiology is unknown, they are likely to be correlated with an abnormal exacerbated immune response to otherwise innocuous stimuli, which is not properly abrogated by the feedback system that normally downregulates the mucosal response to luminal factors (2). Similar to other inflammatory processes, IBD is characterised by an upregulation of the synthesis and release of a variety of pro-inflammatory mediators, such as reactive oxygen species and cytokines, thus influencing mucosal integrity and leading to excessive tissue injury (3,4). Currently, a specific causal treatment of IBD is not available, and the best regimen is the regulation of the oxidant/antioxidant balance.…”

Stress-responsive systems in rat pancreas upon long-term gastric hypochlorhydria and administration of multiprobiotic “Symbiter

“…це, в свою чергу, знімає зі ШКТ та асоційованих органів навантажен-ня з боку патогенної мікрофлори. Показано, що бактерії роду Lactobacillus, які входять до складу мультипробіотика «Симбітер ® », за ра-хунок синтезу Mn-СОД здатні знижувати інтенсивність запальних процесів у кишеч-нику [40]. Дослідженнями групи Lutgendorff et al [41] показано, що мультикомпонентні пробіотики здатні посилювати de novo син-тез основного низькомолекулярного анти-оксиданту клітини -глутатіону -і, таким чином, підвищувати його вміст як у цілому ШКТ, так і конкретно в підшлунковій залозі.…”