Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf

Sun, Qinqin; Jin, Zhu; Cao, Feiwei; Chen, Fengjia

doi:10.1371/journal.pone.0181094

Cited by 29 publications

(25 citation statements)

References 70 publications

(52 reference statements)

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“…Extracts from Chimonanthus nitens Oliv. leaves also inhibited recruitment of neutrophils (and not macrophages), reduced the LPS-induced upregulation of il1b, il6 , and tnfa expression (Sun et al, 2017 ). The protein TtSOD, which inhibited tail wounding-induced neutrophil migration, was also shown to inhibit neutrophil infiltration upon LPS injection (Sheng et al, 2019 ).…”

Section: Inflammatory Disease Models In Zebrafishmentioning

confidence: 99%

“…In the LPS-induced inflammation model, GC administration was reported to inhibit the production of ROS and NO, the expression of pro-inflammatory genes, the recruitment of leukocytes, and the mortality (Wijesinghe et al, 2014 ; Yang et al, 2014 , 2017 ; Sun et al, 2017 ). In the Copper-induced inflammation model using CuSO 4 immersion of larvae, GCs also caused inhibition of neutrophil accumulation (d'Alençon et al, 2010 ).…”

Section: The Use Of Zebrafish Inflammation Models For Research On Glumentioning

confidence: 99%

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Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs

Xie

Meijer

Schaaf

2021

Front. Cell Dev. Biol.

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Dysregulation of the inflammatory response in humans can lead to various inflammatory diseases, like asthma and rheumatoid arthritis. The innate branch of the immune system, including macrophage and neutrophil functions, plays a critical role in all inflammatory diseases. This part of the immune system is well-conserved between humans and the zebrafish, which has emerged as a powerful animal model for inflammation, because it offers the possibility to image and study inflammatory responses in vivo at the early life stages. This review focuses on different inflammation models established in zebrafish, and how they are being used for the development of novel anti-inflammatory drugs. The most commonly used model is the tail fin amputation model, in which part of the tail fin of a zebrafish larva is clipped. This model has been used to study fundamental aspects of the inflammatory response, like the role of specific signaling pathways, the migration of leukocytes, and the interaction between different immune cells, and has also been used to screen libraries of natural compounds, approved drugs, and well-characterized pathway inhibitors. In other models the inflammation is induced by chemical treatment, such as lipopolysaccharide (LPS), leukotriene B4 (LTB4), and copper, and some chemical-induced models, such as treatment with trinitrobenzene sulfonic acid (TNBS), specifically model inflammation in the gastro-intestinal tract. Two mutant zebrafish lines, carrying a mutation in the hepatocyte growth factor activator inhibitor 1a gene (hai1a) and the cdp-diacylglycerolinositol 3-phosphatidyltransferase (cdipt) gene, show an inflammatory phenotype, and they provide interesting model systems for studying inflammation. These zebrafish inflammation models are often used to study the anti-inflammatory effects of glucocorticoids, to increase our understanding of the mechanism of action of this class of drugs and to develop novel glucocorticoid drugs. In this review, an overview is provided of the available inflammation models in zebrafish, and how they are used to unravel molecular mechanisms underlying the inflammatory response and to screen for novel anti-inflammatory drugs.

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Section: Inflammatory Disease Models In Zebrafishmentioning

confidence: 99%

Section: The Use Of Zebrafish Inflammation Models For Research On Glumentioning

confidence: 99%

Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs

Xie

Meijer

Schaaf

2021

Front. Cell Dev. Biol.

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“…Studies on Ch. nitens have focused on its calycanthine, chimonanthine, coumarins, flavonoids, phenolic acids, terpenoids, and volatile oils [45,46,47] and their pharmacologies (anti-inflammatory properties [48], antihyperglycaemic [49] and antihyperlipidaemic efficacies, and antioxidant capacity [50], inhibitory α-glucosidase activity [51], and toxicity [52]), phylogeography [45,53], and fingerprints [54,55]. Studies on Ch.…”

Section: Introductionmentioning

confidence: 99%

Physical Map of FISH 5S rDNA and (AG3T3)3 Signals Displays Chimonanthus campanulatus R.H. Chang & C.S. Ding Chromosomes, Reproduces its Metaphase Dynamics and Distinguishes Its Chromosomes

Luo

Chen

2019

Genes

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Chimonanthus campanulatus R.H. Chang & C.S. Ding is a good horticultural tree because of its beautiful yellow flowers and evergreen leaves. In this study, fluorescence in situ hybridization (FISH) was used to analyse mitotic metaphase chromosomes of Ch. campanulatus with 5S rDNA and (AG3T3)3 oligonucleotides. Twenty-two small chromosomes were observed. Weak 5S rDNA signals were observed only in proximal regions of two chromosomes, which were adjacent to the (AG3T3)3 proximal signals. Weak (AG3T3)3 signals were observed on both chromosome ends, which enabled accurate chromosome counts. A pair of satellite bodies was observed. (AG3T3)3 signals displayed quite high diversity, changing in intensity from weak to very strong as follows: far away from the chromosome ends (satellites), ends, subtelomeric regions, and proximal regions. Ten high-quality spreads revealed metaphase dynamics from the beginning to the end and the transition to anaphase. Chromosomes gradually grew larger and thicker into linked chromatids, which grew more significantly in width than in length. Based on the combination of 5S rDNA and (AG3T3)3 signal patterns, ten chromosomes were exclusively distinguished, and the remaining twelve chromosomes were divided into two distinct groups. Our physical map, which can reproduce dynamic metaphase progression and distinguish chromosomes, will powerfully guide cytogenetic research on Chimonanthus and other trees.

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“…Chimonanthus contains volatile oil, coumarins, alkaloids, alkene, sesquiterpenoids, flavonoids [3][4][5][6]. The main medicinal components are alkaloids [7][8][9][10]. Calycanthine is an important class of alkaloids extracted and isolated from the roots, leaves, flowers and fruits of Chimonanthus praecox [11,12].…”

Section: Introductionmentioning

confidence: 99%

Determination and pharmacokinetics of calycanthine in rat plasma by UPLC-MS/MS

Zheng

Wen

2020

AChrom

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AbstractCalycanthine is an important class of alkaloids extracted and isolated from the roots, leaves, flowers and fruits of Chimonanthus praecox. In this work, the UPLC-MS/MS method was used for determination of calycanthine in rat plasma, and the pharmacokinetics in rats were investigated. Midazolam was used as an internal standard (IS), and methanol precipitation method was used to pretreatment the rat plasma samples. Chromatographic separation was achieved on a UPLC BEH C18 (50 × 2.1 mm, 1.7 μm) column with the mobile phase of methanol- 0.1% formic acid aqueous solution with gradient elution. Multiple reaction monitoring (MRM) mode with positive ionization was applied for quantitative analysis, m/z 347.3 → 246.7 and 326.2 → 291.4 for calycanthine and IS, respectively. The results indicated that within the range of 1–200 ng/mL, linearity of calycanthine in rat plasma was good (r > 0.995), and the lower limit of quantification (LLOQ) was 1 ng/mL. Accuracy range was between 90.6 and 109.4%, precision (RSD) of calycanthine was less than 14%. The matrix effect was between 97.9% and 105.4%, the recovery was better than 85.6%. The developed UPLC-MS/MS method was successfully applied in the pharmacokinetics of calycanthine in rats after oral and intravenous administration. The absolute bioavailability of the calycanthine was 37.5% in rats.

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Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf

Cited by 29 publications

References 70 publications

Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs

Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs

Physical Map of FISH 5S rDNA and (AG3T3)3 Signals Displays Chimonanthus campanulatus R.H. Chang & C.S. Ding Chromosomes, Reproduces its Metaphase Dynamics and Distinguishes Its Chromosomes

Determination and pharmacokinetics of calycanthine in rat plasma by UPLC-MS/MS

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