Anti-inflammatory properties of Ajuga bracteosa in vivo and in vitro study and their effects on mouse model of liver fibrosis

Hsieh, Wen Tsong; Liu, Yu Ting; Lin, Wen

doi:10.1016/j.jep.2011.02.031

Cited by 25 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are supported by histological findings, as HE staining revealed that MLT was able to reduce necrosis, hepatocyte degeneration, and the extensive presence of the inflammatory infiltrate, as a result of exposure to CCl 4, showing further evidence of the hepatoprotective effect of MLT. Our findings are in agreement with previous studies showing that the use of MLT, as well as other substances with antioxidant power, reduces the expression of inflammatory mediators during fibrogenesis and hepatocarcinogenesis induced by hepatotoxins . Studies have shown that the treatment with MLT reduces the release of NO in the vasculature and attenuates iNOS expression in the liver, as demonstrated in models of sepsis, ischemia/reperfusion, cholestasis, ionizing radiation, and liver injury caused by toxins such as aflatoxin, CCl 4 , methanol, and thioacetamide …”

Section: Discussionsupporting

confidence: 93%

“…The CCl 4 induction of oxidative damage stimulated IkB phosphorylation, which releases NF-κB p65 to act in the nucleus, with an influence on the transcription of target genes and resulting in the production and secretion of pro-inflammatory cytokines implicated in promoting fibrosis. [36][37][38] Once activated, NF-kB stimulates the expression of iNOS with the consequent increase in the production of nitric oxide (NO), a molecule highly involved in toxin-induced liver injury. 38 Similar findings were found in our study, where CCl 4 may have induced IkB phosphorylation based on the increased nuclear expression of NF-κB p65, as well as the concomitant increase in the expression of iNOS.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride

et al. 2018

View full text Add to dashboard Cite

Background and AimLiver diseases are a major public health problem, accounting for a significant number of hospital visits and admissions and an increasing mortality rate. Melatonin (MLT) is a powerful antioxidant molecule that has been shown to be beneficial under various conditions. The objective was to evaluate the effect of MLT on experimental liver cirrhosis induced by carbon tetrachloride (CCl4) in rats.MethodsTwenty male Wistar rats (230–250 g) were divided into four groups. I: control group (CO); II: CO + MLT; III: CCl4; and IV: CCl4 + MLT. CCl4 was administered intraperitoneally (i.p.) as follows: 10 doses every 5 days, 10 doses every 4 days, and 7 doses every 3 days. MLT was administered i.p. at a dose of 20 mg/kg from the 10th week to the end of the experiment (16th week).ResultsIn the CCl4 + MLT group, we found that MLT caused a decrease in the level of F2‐isoprostanes and NQO1 expression. We also found that MLT reduced the inflammatory process as shown by decreased expressions of NF‐KB/p65 and inducible nitric oxide synthase (iNOS) and a smaller amount of inflammatory infiltrate. MLT reduced the expression of transforming growth factor beta1 (TGF‐β1), alpha‐smooth muscle actin (α‐SMA), and vascular endothelial growth factor (VEGF). Picrosirius staining showed that MLT decreases fibrosis.ConclusionMLT has a potent antifibrogenic effect, modulating the parameters of oxidative stress, angiogenesis, and inflammation.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The histological results showed that the normal structure of lobules was destroyed, and pseudolobules were formed. Moreover, the increased hydroxyproline content in liver, the key characteristic component of collagen [31], also confirmed the hepatic fibrogenesis in rats. There was a significant increase in the levels of ALT, AST, GGT, TBil as well as decrease in serum Alb content on exposure to CCl 4 , indicating considerable hepatocellular injury.…”

Section: Discussionmentioning

confidence: 93%

Urine metabolic profile changes of CCl4-liver fibrosis in rats and intervention effects of Yi Guan Jian Decoction using metabonomic approach

Gou

Tao²,

Peng³

et al. 2013

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundYi Guan Jian Decoction (YGJD), a famous Chinese prescription, has long been employed clinically to treat liver fibrosis. However, as of date, there is no report on the effects of YGJD from a metabonomic approach. In this study, a urine metabonomic method based on gas chromatography coupled with mass spectrometry (GC/MS) was employed to study the protective efficacy and metabolic profile changes caused by YGJD in carbon tetrachloride (CCl4)-induced liver fibrosis.MethodsUrine samples from Wistar rats of three randomly divided groups (control, model, and YGJD treated) were collected at various time-points, and the metabolic profile changes were analyzed by GC/MS with principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA). Furthermore, histopathology and biochemical examination were also carried out to ensure the success of CCl4-induced liver fibrosis model.ResultsUrine metabolic profile studies suggested distinct clustering of the three groups, and YGJD group was much closer to the control group by showing a tendency of recovering towards the control group. Fourteen significantly changed metabolites were found, and YGJD treatment could reverse the levels of these metabolites to normal levels or close to normal levels.ConclusionsThe current study indicates that the YGJD has significant anti-fibrotic effects on CCl4-induced liver fibrosis in rats, which might be by regulating the dysfunction of energy metabolism, amino acid metabolism, tryptophan metabolism, cytochrome P450 metabolism, and gut microflora metabolism. The metabonomic approach can be recommended to study the pharmacological effect and mechanism of complex Chinese medicines.

show abstract

“…This plant is also renowned for its astringent, febrifugal, stimulant and diuretic properties [46-48]. Ajuga bracteosa contains sphingolides, bractic acid, diterpenoids and withanolides which inhibit enzymes such as lipoxygenase (LOX) and acetylcholinesterase (AChE) [47]. Scientific studies revealed chemopreventitive, antiplasmodial and cardiostimulant effects [46; 48].…”

Section: Iridoid Chemistry and Researched Anti-inflammatory Iridoid-cmentioning

confidence: 99%

Anti-Inflammatory Iridoids of Botanical Origin

Viljoen

Mncwangi²,

Vermaak³

2012

CMC

174

110

View full text Add to dashboard Cite

Inflammation is a manifestation of a wide range of disorders which include; arthritis, atherosclerosis, Alzheimer’s disease, inflammatory bowel syndrome, physical injury and infection amongst many others. Common treatment modalities are usually non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin, paracetamol, indomethacin and ibuprofen as well as corticosteroids such as prednisone. These however, may be associated with a host of side effects due to non-selectivity for cyclooxygenase (COX) enzymes involved in inflammation and those with selectivity may be highly priced. Thus, there is a continuing search for safe and effective anti-inflammatory molecules from natural sources. Research has confirmed that iridoids exhibit promising anti-inflammatory activity which may be beneficial in the treatment of inflammation. Iridoids are secondary metabolites present in various plants, especially in species belonging to the Apocynaceae, Lamiaceae, Loganiaceae, Rubiaceae, Scrophulariaceae and Verbenaceae families. Many of these ethnobotanicals have an illustrious history of traditional use alluding to their use to treat inflammation. Although iridoids exhibit a wide range of pharmacological activities such as cardiovascular, hepatoprotection, hypoglycaemic, antimutagenic, antispasmodic, anti-tumour, antiviral, immunomodulation and purgative effects this review will acutely focus on their anti-inflammatory properties. The paper aims to present a summary for the most prominent iridoid-containing plants for which anti-inflammatory activity has been demonstrated in vitro and / or in vivo.

show abstract

Anti-inflammatory properties of Ajuga bracteosa in vivo and in vitro study and their effects on mouse model of liver fibrosis

Cited by 25 publications

References 29 publications

Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride

Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride

Urine metabolic profile changes of CCl4-liver fibrosis in rats and intervention effects of Yi Guan Jian Decoction using metabonomic approach

Anti-Inflammatory Iridoids of Botanical Origin

Contact Info

Product

Resources

About