Anti-inflammatory Potential of Saponins from <i>Aster tataricus</i> via NF-κB/MAPK Activation

Su, Xiang Dong; Jang, Hyun‐Jae; Wang, Cai-Yi; Lee, Seung Woong; Rho, Mun‐Chual; Kim, Young Ho; Yang, Seo Young

doi:10.1021/acs.jnatprod.8b00856

Cited by 30 publications

(34 citation statements)

References 32 publications

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“…During the process of pulmonary diseases, inflammatory reaction plays an important role (Welbourn and Young, 1992; Ghio and Devlin, 2001; Ricard et al, 2001; Peng et al, 2004; Menezes et al, 2005). Many preliminary studies confirmed the anti-inflammatory and immune-regulatory effects of natural products, while several data showed a decreased effect of their metabolites on cytokines for inflammation, such as IL-1, IL-6, and TNF-α (Teng et al, 2016, Teng et al, 2017; Chen et al, 2018, Chen et al, 2019; Gupta et al, 2019; Magalhães et al, 2019; Saudagar and Saokar, 2019; Su et al, 2019; Zhang et al, 2019). Hence, it arouses our interest that whether MXD, a famous traditional Chinese medicine formula widely used in respiratory diseases, can alleviate PM2.5 induced lung injury.…”

Section: Discussionmentioning

confidence: 93%

Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat

et al. 2019

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Ma Xing Shi Gan Decoction (MXD), a classical traditional Chinese medicine prescription, is widely used for the treatment of upper respiratory tract infection. However, the effect of MXD against particulate matters with diameter of less than 2.5 μm (PM2.5) induced lung injury remains to be elucidated. In this study, rats were stimulated with PM2.5 to induce lung injury. MXD was given orally once daily for five days. Lung tissues were harvested to assess pathological changes and edema. Myeloperoxidase (MPO) activity and malonaldehyde (MDA) content in lung were determined to evaluate the degree of injury. To assess the barrier disruption, the bronchoalveolar lavage fluid (BALF) was collected to determine the total protein content and count the number of neutrophils and macrophages. For evaluating the activation of macrophage in lung tissue, CD68 was detected using immunohistochemistry (IHC). The levels of inflammatory factors including tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and interleukin-6 (IL-6) in BALF and serum were measured. In vitro, a PM2.5-activated RAW 264.7 macrophages inflammatory model was introduced. To evaluate the protective effect of MXD-medicated serum, the cell viability and the release of inflammatory factors were measured. The effects of MXD on the High mobility group box-1/Toll-like receptor 4/Nuclear factor-kappa B (HMGB1/TLR4/NFκB) pathway in lung tissue and RAW 264.7 cells were assessed by Western blot. For further confirming the protective effect of MXD was mediated by inhibiting the HMGB1/TLR4/NFκB pathway, RAW 264.7 cells were incubated with MXD-medicated serum alone or MXD-medicated serum plus recombinant HMGB1 (rHMGB1). MXD significantly ameliorated the lung injury in rats, as evidenced by decreases in the pathological score, lung edema, MPO activity, MDA content, CD68 positive macrophages number, disruption of alveolar capillary barrier and the levels of inflammatory factors. In vitro, MXD-medicated serum increased cell viability and inhibited the release of inflammatory cytokines. Furthermore, MXD treatment was found to inhibit HMGB1/TLR4/NFκB signal pathway both in vivo and in vitro. Moreover, the protection of MXD could be reversed by rHMGB1 in RAW 264.7. Taken together, these results suggest MXD protects rats from PM2.5 induced acute lung injury, possibly through the modulation of HMGB1/TLR4/NFκB pathway and inflammatory responses.

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Section: Discussionmentioning

confidence: 93%

Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat

et al. 2019

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“…RAW 264.7 (ATCC TIB-71) cells were cultured in Dulbecco’s modified Eagle medium (DMEM) and NO assay was carried out for measurements of NO release using previously reported method [46]. Briefly, RAW 264.7 cells were plated at 1 × 10 5 cell density in a 96-well microplate and cultured for 24 h. Compounds ( 1 – 22 ) were pretreated with increasing dose concentrations (0.5, 1, 5, 10, 25, 50, and 100 μM), and then stimulated with LPS (1 μg/mL, Sigma–Aldrich, St. Louis, MO, USA) for 18 h. The mixture of cell supernatant (100 μL) and Griess reagent [1% sulfanilamide + 0.1% N -(1-naphthyl)ethylenediamine (Sigma–Aldrich)] in 5% phosphoric acid was recorded at 550 nm using a microplate reader (Varioskan LUX, Thermo Fisher Scientific Inc., Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

1-Carbomethoxy-β-Carboline, Derived from Portulaca oleracea L., Ameliorates LPS-Mediated Inflammatory Response Associated with MAPK Signaling and Nuclear Translocation of NF-κB

Kim¹,

Park

Jang³

et al. 2019

Molecules

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Portulaca oleracea is as a medicinal plant known for its neuroprotective, hepatoprotective, antidiabetic, antioxidant, anticancer, antimicrobial, antiulcerogenic, and anti-inflammatory activities. However, the specific active compounds responsible for the individual pharmacological effects of P. oleracea extract (95% EtOH) remain unknown. Here, we hypothesized that alkaloids, the most abundant constituents in P. oleracea extract, are responsible for its anti-inflammatory activity. We investigated the phytochemical substituents (compounds 1–22) using nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS) and screened their effects on NO production in lipopolysaccharide (LPS)-induced macrophages. Compound 20, 1-carbomethoxy-β-carboline, as an alkaloid structure, ameliorated nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), and proinflammatory cytokines associated with the mitogen-activated protein kinase (MAPK) pathways, p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). Subsequently, we observed that compound 20 suppressed nuclear translocation of nuclear factor κB (NF-κB) using immunocytochemistry. Moreover, we recently reported that compound 8, trans-N-feruloyl-3’, 7’-dimethoxytyramine, was originally purified from P. oleracea extracts. Our results suggest that 1-carbomethoxy-β-carboline, the most effective anti-inflammatory agent among alkaloids in the 95% EtOH extract of P. oleracea, was suppressing the MAPK pathway and nuclear translocation of NF-κB. Therefore, P. oleracea extracts and specifically 1-carbomethoxy-β-carboline may be novel therapeutic candidates for the treatment of inflammatory diseases associated with the activation of MAPKs and NF-κB.

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“…5B) 6F), which was restored in ATE (100 mg/kg and 500 mg/kg)-treated groups significantly (p<0.05). Traditionally, A. tataricus is used for medicinal and several reports have indicated the presence of numerous bioactive constituents including dietary flavonoids, triterpenes, saponins and specific constituents such as epifriedelinol, caffeoylquinic acids, shionone and aster peptides shionone, epifriedelinol, quercetin, kaempferol, scopoletin, emodin, aurantiamide acetate and 1,7-dihydroxy-6-methyl-anthraquinone [49][50][51][52] . Some of these compounds, such as quercetin, saponins, kaempferol, epifriedelinol, caffeoylquinic acids possess strong antioxidant, antiinflammatory and hepatoprotective properties including antifibrotic effects [53][54][55][56] .…”

Section: Fig 1: Effect Of Ate In Cell Viability Assay On Chang Livermentioning

confidence: 99%

“…A. tataricus was also reported to have antioxidant activity and reduce the levels of alanine aminotransferase (ALS), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in hepatotoxic effect induced by Fr-2 [18,19] . More recently, potential inhibitors were isolated from Aster tataricuswhich exhibited antiinflammatory effects bypreventing the downstream activation of the mitogen-activated protein kinase signaling pathway [20,21] . A. tataricus was found to protect retinal damage in diabetic rats due to the antioxidant and antiinflammatory effects [22] and attenuated neuroinflammation by decreasing the oxidative stress and inhibiting the cytokine production in LPS-stimulated rat astrocytoma cell line (C6) and THP-1 cells [23] .…”

mentioning

confidence: 99%

Aster tataricus Linn., Suppresses Hepatic Stellate Cell Activation and Protects Against Thioacetamide-induced Liver Fibrosis in Rats

Kim¹,

Koppula²,

Yum³

et al. 2020

ijps

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Kim et al.: Antihepatofibrotic Effect of Aster tataricusAster tataricus Linn., (Asteraceae), an oriental and nutrient-potential herb is used in Asian countries for various health benefits. The present study focused on the protective effects of Aster tataricus against liver fibrosis in cellular and an experimental rat model. Cell cytotoxicity, cell cycle and apoptosis functions were analyzed using hepatic stellate cell lines following MTT assay, flow cytometry, and Annexin V-FITC/PI staining methods. For in vivo evaluation, thioacetamide-induced hepatofibrosis rat model was established. Sprague Dawley rats were divided into 5 groups of 10 each (control, thioacetamide, thioacetamide with Aster tataricus extract 100 and 500 mg/kg and silymarin 50 mg/kg groups, respectively). Fibrosis was induced by thioacetamide treatment (200 mg/kg, ip) 3 times per week for 13 weeks except for the control group. Aster tataricus extract (100 and 500 mg/kg), and silymarin was administrated orally to each group 6 times a week from week 7 to 13 and various fibrosis-related parameters were estimated using real-time polymerase chain reaction using TRIzol Plus RNA purification Kit and a spectrophotometer. Results indicated that hepatic stellate cells treated with Aster tataricus extract (0.5 mg/ml) and silymarin (0.05 mg/ml) significantly (p<0.05) induced apoptosis (19.04 and 24.82 %, respectively) compared to the control group (9.78 %). Moreover, rat primary hepatic stellate cells showed morphological changes and degradation of collagen and fibronectin when treated with 0.5 mg/kg of Aster tataricus extract. In vivo evaluation Aster tataricus extract at concentrations of 100 and 500 mg/mlattenuated the increased serum levels of alanine transaminase, aspartate transaminase and hydroxyproline and restored the decreased glutathione levels significantly in thioacetamide induced fibrotic rats (p<0.05). The altered histopathology in thioacetamide-induced liver tissues and changes in fibrosis-related gene expression (TGF-β, α-SMA and Col1α1) were also restored by Aster tataricus extract treatment. In conclusion, Aster tataricus can be developed as a potential therapeutic agent in the treatment of liver fibrosis.

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Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation

Cited by 30 publications

References 32 publications

Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat

Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat

1-Carbomethoxy-β-Carboline, Derived from Portulaca oleracea L., Ameliorates LPS-Mediated Inflammatory Response Associated with MAPK Signaling and Nuclear Translocation of NF-κB

Aster tataricus Linn., Suppresses Hepatic Stellate Cell Activation and Protects Against Thioacetamide-induced Liver Fibrosis in Rats

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