Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF-��B and MAPKs Pathways in LPS-Induced RAW 264.7 Cells

Abstract

Probiotics are known to provide the host with immune-modulatory effects and are therefore of remarkable interest for therapeutic and prophylactic applications against various disorders, including inflammatory diseases. Weissella cibaria JW15 (JW15) has been reported to possess probiotic and antioxidant properties. However, the effect of JW15 on inflammatory responses has not yet been reported. Therefore, the objective of the current study was to evaluate the anti-inflammatory potential of JW15 against lipopolysaccharide (LPS) stimulation. The production of pro-inflammatory factors and the cellular signaling pathways following treatment with heat-killed JW15 was examined in LPS-induced RAW 264.7 cells. Treatment with heat-killed JW15 decreased nitric oxide and prostaglandin E 2 production via downregulation of the inducible nitric oxide synthase and cyclooxygenase-2. In addition, treatment with heat-killed JW15 suppressed the expression of pro-inflammatory cytokines, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. The anti-inflammatory properties of treating with heat-killed JW15 were associated with mitogen-activated protein kinase signaling pathwaymediated suppression of nuclear factor-κB. These results indicated that JW15 possesses antiinflammatory potential and provide a molecular basis regarding the development of functional probiotic products.

Weissella cibaria CMU exerts an anti‑inflammatory effect by inhibiting Aggregatibacter actinomycetemcomitans‑induced NF‑κB activation in macrophages

“…The present study used inactivated A. actinomycetemcomitans as a trigger instead of its LPS and found that live oraCMU decreased A. actinomycetemcomitans-induced NO production in RAW 264.7 cells by inhibiting iNOS at the mRNA level. Similarly, Yu et al (31), reported that treatment with heat-inactivated W. cibaria JW15 decreased NO production in RAW 264.7 cells upon LPS stimulation, which was attributable to downregulated iNOS expression.…”

“…7). Heat-inactivated W. cibaria JW15 has also been shown to suppress IL1β and IL6 expression; moreover, mechanistically, its anti-inflammatory properties are mediated by mitogen-activated protein kinase signaling and result in NF-κB inhibition (31).…”

Inula britannica Inhibits Adipogenesis of 3T3-L1 Preadipocytes via Modulation of Mitotic Clonal Expansion Involving ERK 1/2 and Akt Signaling Pathways

“…Western blotting analysis was performed as described previously with a minor modification [ 31 ]. The 3T3-L1 preadipocytes plated in 60 mm culture dishes (1.0 × 10 5 cells/dish) were incubated and differentiated for various intervals in the presence of different concentrations of IAE.…”

“…The qRT-PCR analysis was performed as described previously [ 31 ]. The 3T3-L1 preadipocytes seeded in 6-well culture plates (5 × 10 4 cells/well) were differentiated until day 8, as described in Section 2.3 .…”

Effect of a Synbiotic Containing Lactobacillus paracasei and Opuntia humifusa on a Murine Model of Irritable Bowel Syndrome

“…We used L. paracasei DKGF 1 as a probiotic, and O. humifusa extract as a prebiotic. L. paracasei DKGF 1 was isolated from Kimchi, cultivated and maintained in MRS (Man, Rogosa, and Sharpe; Difco Laboratories, Detroit, MI, USA) at 37 °C [ 24 , 25 ]. O. humifusa , locally called as Cheonnyuncho, is widely cultivated in the southern coastal regions of South Korea.…”