Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages

Arulselvan, Palanisamy; Tan, Woan Sean; Gothai, Sivapragasam; Muniandy, Katyakyini; Fakurazi, Sharida; Esa, Norhaizan Mohd; Alarfaj, Abdullah A.; Kumar, Suresh

doi:10.3390/molecules21111452

Cited by 66 publications

(58 citation statements)

References 35 publications

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“…Our study showed that the control LPS-induced cells significantly increase in iNO, PGE 2 , IL1β and IL10 productions and TNF-α non-significantly in comparison to the normal cells. This was in agreement with other studies confirming the activation of signalling pathways, which leads to the release of pro-inflammatory cytokines, including IL1 β, TNF-α and mediators like NO and PGE 2 and anti-inflammatory cytokines like IL10 induced by LPS in RAW macrophage cells in the study [ 24 , 25 , 26 ]. The positive controls LNAME and quercetin in our study significantly reversed these effects.…”

Section: Discussionsupporting

confidence: 93%

Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

Nkadimeng

Nabatanzi

Steinmann

et al. 2020

Plants

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Psilocybin-containing mushrooms, commonly known as magic mushrooms, have been used since ancient and recent times for depression and to improve quality of life. However, their anti-inflammatory properties are not known. The study aims at investing cytotoxicity; antioxidant; and, for the first time, anti-inflammatory effects of Psilocybe natalensis, a psilocybin-containing mushroom that grows in South Africa, on lipopolysaccharide-induced RAW 264.7 macrophages. Macrophage cells were stimulated with lipopolysaccharide and treated with different concentrations of Psilocybe natalensis mushroom extracted with boiling hot water, cold water and ethanol over 24 h. Quercetin and N-nitro-L-arginine methyl ester were used as positive controls. Effects of extracts on the lipopolysaccharide-induced nitric oxide, prostaglandin E2, and cytokine activities were investigated. Phytochemical analysis, and the antioxidant and cytotoxicity of extracts, were determined. Results showed that the three extracts inhibited the lipopolysaccharide-induced nitric oxide, prostaglandin E2, and interleukin 1β cytokine production significantly in a dose-dependent manner close to that of the positive controls. A study proposed that ethanol and water extracts of Psilocybe natalensis mushroom were safe at concentrations used, and have antioxidant and anti-inflammatory effects. Phytochemical analysis confirmed the presence of natural antioxidant and anti-inflammatory compounds in the mushroom extracts.

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Section: Discussionsupporting

confidence: 93%

Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

Nkadimeng

Nabatanzi

Steinmann

et al. 2020

Plants

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“…The results demonstrated that the MO extract strongly inhibited the LPS-induced expression of IL-6 and TNF-α during inflammation while increasing anti-inflammatory cytokine IL-10. Our present findings are consistent with a previous study fraction [17], which investigated the effects of different solvent fractions, including butanol, ethyl acetate, chloroform, and hexane of MO extract. The authors observed a decrease in IL-6, TNF-α, IL-1β, and prostaglandin E2 (PGE2) production in LPS-stimulated RAW264.7 macrophages after treatment with each solvent.…”

Section: Discussionsupporting

confidence: 93%

“…In Malaysia, fraction of MO leaves have been reported to be anti-inflammatory, by inhibiting Lipopolysaccharide (LPS)-induced production of nitric oxide and the pro-inflammatory cytokines in RAW264.7 cells [17]. Another study identified that isothiocyanates, bioactive from MO leaves extract, significantly inhibited the expression of iNOS, IL-1β, and the production of NO and TNF-β [18].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Compounds in Moringa oleifera Lam. Leaves Inhibit the Pro-Inflammatory Mediators in Lipopolysaccharide-Induced Human Monocyte-Derived Macrophages

et al. 2020

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Moringa oleifera (MO) is an important plant for traditional medicine. The present study aimed to identify the MO active phytochemical compounds for their ability against inflamed macrophages. An ethyl acetate extract fraction of MO was fractionation by flash column chromatography. Human macrophages were stimulated by Lipopolysaccharide and then treated with fractions of MO to examine their anti-inflammatory activity and cellular mechanism. The active fractions were analyzed by liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometer (LC-ESI-QTOF-MS). MO treated cells showed a decreased production of pro-inflammatory mediator in response to lipopolysaccharide. This was evident at both mRNA and protein levels. The study revealed that MO suppressed mRNA expression of IL-1, IL-6, TNF-α, PTGS2, NF-κB (P50), and RelA. Furthermore, the extract effectively inhibited the expression of inflammatory mediators, including IL-6, TNF-α, and cyclooxygenase-2. Interestingly, the effect of MO inhibited phosphorylation of IκB-α and the ability to reduce expression of the nuclear factor (NF)-κB p65, suppressing its nuclear translocation. Moreover, LC-ESI-QTOF-MS analysis of the MO active fraction revealed seven compounds, namely 3,4-Methyleneazelaic acid, (2S)-2-phenylmethoxybutane-1,4-diol, (2R)-2-phenylmethoxybutane-1, 4-diol, γ-Diosphenol, 2,2,4,4-Tetramethyl-6-(1-oxobutyl)-1,3,5-cyclohexanetrione, 3-Hydroxy-β-ionone, and Tuberonic acid. Our findings highlight the ability of MO compounds to inhibit inflammation through regulation of the NF-κB pathway.

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“…The fruit demonstrated the highest activity in reducing NO release induced by LPS in RAW264.7 cells (Lee et al, ). The fruit extract of MO blocked the atomic translocation of NF‐kB and increased inhibitor kB articulation (Arulselvan et al, ). MO roots showed evidence of restraint against IL‐2 activity (Sashidara et al, ).…”

Section: Resultsmentioning

confidence: 99%

Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

Dhakad

Ikram

Sharma

et al. 2019

Phytotherapy Research

169

100

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The genus Moringa Adans. comprises 13 species, of which Moringa oleifera Lam. native to India and cultivated across the world owing to its drought and frost resistance habit is widely used in traditional phytomedicine and as rich source of essential nutrients. Wide spectrum of phytochemical ingredients among leaf, flower, fruit, seed, seed oil, bark, and root depend on cultivar, season, and locality. The scientific studies provide insights on the use of M. oleifera with different aqueous, hydroalcoholic, alcoholic, and other organic solvent preparations of different parts for therapeutic activities, that is, antibiocidal, antitumor, antioxidant, anti‐inflammatory, cardio‐protective, hepato‐protective, neuro‐protective, tissue‐protective, and other biological activities with a high degree of safety. A wide variety of alkaloid and sterol, polyphenols and phenolic acids, fatty acids, flavanoids and flavanol glycosides, glucosinolate and isothiocyanate, terpene, anthocyanins etc. are believed to be responsible for the pragmatic effects. Seeds are used with a view of low‐cost biosorbent and coagulant agent for the removal of metals and microbial contamination from waste water. Thus, the present review explores the use of M. oleifera across disciplines for its prominent bioactive ingredients, nutraceutical, therapeutic uses and deals with agricultural, veterinarian, biosorbent, coagulation, biodiesel, and other industrial properties of this “Miracle Tree.”

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Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages

Cited by 66 publications

References 35 publications

Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

Bioactive Compounds in Moringa oleifera Lam. Leaves Inhibit the Pro-Inflammatory Mediators in Lipopolysaccharide-Induced Human Monocyte-Derived Macrophages

Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

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