Anti-inflammatory ketoprofen in the treatment of field cases of bovine mastitis

Shpigel, Nahum Y.; Chen, R.; Winkler, M.; Saran, A.; Ziv, G.; Longo, Fabio

doi:10.1016/0034-5288(94)90197-x

Cited by 41 publications

(43 citation statements)

References 7 publications

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“…Therefore, the study design included carprofen administration following the appearance of initial clinical symptoms (Shpigel et al, 1994) and inflammatory dynamics of the moderate inflammatory model used (Vangroenweghe et al, 2004a,b). It is known that pretreatment is more effective in inhibiting the inducible COX-2 enzyme (Burvenich, 1985;Burvenich et al, 1989); however, from a practical point of view, the earliest occasion at which field cases of clinical E. coli mastitis can be diagnosed and subsequently treated, occurs at the milking following the infection of the mammary quarter (Shpigel et al, 1994(Shpigel et al, , 1996. The first clinical signs (RT > 40°C, quarter swelling, slightly abnormal milk appearance) in the present experimental model, using a 1 × 10 4 cfu inoculum dose, appeared around PIH 9.…”

Section: Discussionmentioning

confidence: 99%

“…Nonsteroidal anti-inflammatory drugs (NSAID) with antipyretic, analgesic, and anti-inflammatory activities, have been used as adjunctive or alternative therapy to systemic or intramammary antibiotics (Shpigel et al, 1994). Nonsteroidal anti-inflammatory drugs have been reported to alter the clinical course of E. coli or endotoxin-induced mastitis in the bovine.…”

Section: Introductionmentioning

confidence: 99%

“…In these studies, NSAIDs positively affected various clinical, hematological, biochemical, and pathophysiological parameters. Recently, ketoprofen, phenylbutazone, and dipyrone were shown to have beneficial effects in the treatment of field cases of clinical mastitis (Shpigel et al, 1994(Shpigel et al, , 1996. However, these beneficial effects were claimed using NSAID treatment against a historical control group, which could raise questions with respect to a possible confusion between treatment effects and temporal effects.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Carprofen Treatment Following Experimentally Induced Escherichia coli Mastitis in Primiparous Cows

Vangroenweghe

Duchateau

Boutet

et al. 2005

Journal of Dairy Science

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Acute Escherichia coli mastitis is one of the major sources of economic loss in the dairy industry due to reduced milk production, treatment costs, discarded milk, and occasional fatal disease. Nonsteroidal antiinflammatory drugs (NSAIDs) are frequently used as adjunctive therapy to antibiotics. The objective of the current study was to evaluate the effect of carprofen treatment following infusion of Escherichia coli into the mammary glands of primiparous cows during the periparturient period. Severity of mastitis was scored based on the average milk production in the uninfected quarters on d +2 postinoculation and a clinical severity score. Carprofen was administered intravenously at 9 h postchallenge, when clinical signs of mastitis appeared. In previous work, efficacy of NSAIDs was mainly evaluated using clinical symptoms. In the present study, the effect of carprofen on innate immune response was also assessed by quantification of inflammatory mediators. All primiparous cows reacted as moderate responders throughout the experimental period. Primiparous cows were intramammarily inoculated with 1 × 10 4 cfu of E. coli P4:O32 in 2 left quarters. Analysis of blood and milk parameters, including IL-8, complement component C5a, lipopolysaccharide-binding protein (LBP), soluble CD14, prostaglandin E 2 , and thromboxane B 2 was performed from d 0 to d +6 relative to intramammary inoculation. Rectal temperature in carprofen-treated animals was lower than in control animals at 3 and 6 h posttreatment. Treatment also restored the decreased reticulorumen motility that occurs during E. coli mastitis to preinfection levels faster than in control animals. Carprofen treatment resulted 2361in an earlier normalization of the clinical severity score. Eicosanoid (prostaglandin E 2 and thromboxane B 2 ) production in milk tended to be inhibited by carprofen. No significant differences in the kinetic patterns of somatic cell count, IL-8, complement component C5a, LBP, and soluble CD14 were observed. In conclusion, carprofen treatment improved general clinical condition by effective antipyrexia and restoration of reticulorumen motility but did not significantly inhibit eicosanoid production. Carprofen treatment did not result in a significant decrease of chemotactic inflammatory mediators, IL-8 and C5a, and early innate immune molecules, sCD14 and LBP. Therefore, major modulatory effects from NSAID administration were not observed in this mastitis model, although a larger study might confirm some apparent trends obtained in the present results. (Key words: primiparous cow, carprofen, Escherichia coli mastitis, moderate inflammation) Abbreviation key: C5a = complement component 5a, COX = cyclo-oxygenase, HR = heart rate, LBP = LPSbinding protein, NSAID = nonsteroidal anti-inflammatory drug, PGE 2 = prostaglandin E 2 , PIH = postinfusion hour, RT = rectal temperature, sCD14 = soluble CD14, TMB = tetramethylbenzidine, TXB 2 = thromboxane B 2 .

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Carprofen Treatment Following Experimentally Induced Escherichia coli Mastitis in Primiparous Cows

Vangroenweghe

Duchateau

Boutet

et al. 2005

Journal of Dairy Science

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“…Also, examining the effects of ketoprofen administration in dairy cows it was concluded that ketoprofen significantly improved recovery in clinical mastitis indicating that ketoprofen did suppress inflammation [12,26].…”

Section: Discussionmentioning

confidence: 99%

Hematological Parameters in Cows in Early Lactation Treated with Ketoprofen and their Relationship with Lipid Mobilization and Ketogenesis

Kovačević

Stojanović

Cincović

et al. 2016

Acta Scientiae. Vet.

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Background: Dairy cows are exposed to numerous hematological and biochemical changes, what is bringing cows into the state of increased metabolic activity and physiological adaptations. These adaptive processes have resulted in increased lipid mobilization and ketogenesis with increased concentration of non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHB). As a non steroidal anti-inflammatory drug, ketoprofen produces anti-inflammatory effects. The main objective of the present study was to found relationship between hematological parameters and ketoprofen administration and high lipid mobilization and ketogenesis in cows treated with ketoprofen immediately after calving. Materials, Methods & Results: Ketoprofen was used (3 mg/kg body weight) intramuscularly for three consecutive days post-partum on 15 cows of Holstein-Friesian breed. Cows of the control group (n = 15) were not treated with ketoprofen. Blood samples were collected at the day of calving, in the first and in the second week after parturition from the coccygeal vein of the both groups. Hematological parameters (erythrocytes, hemoglobin, neutrophils, lymphocytes and mean platelet volume) were determined on the automatic hematological counter. Metabolic parameters (NEFA, BHB) were determined by standard colorimetric kits using a semi-automatic biochemistry analyzer. Student's t-test was used to determine the influence of the application of ketoprofen to the hematological parameters. The data analysis was performed using SPSS, version 19.0, software package for Microsoft Windows (IBM, Armonk, NY, USA). The results of comparison with (P < 0.05) were considered to represent statistically significant differences. Difference in correlation test between NEFA and BHB and other blood parameters in experimental and control group of cows were determined by the Fischer r-to-z transformation. The research results show that there was a increased red blood cell count and a higher concentration of hemoglobin (P < 0.05), fewer neutrophils (P < 0.01), lower number of lymphocytes (P < 0.01) and fewer mean platelet volume in cows that received ketoprofen after calving than those of the control. Determined hematological parameters and values of NEFA and BHB are in correlation. The correlation coefficients were significantly lower in the experimental group of cows. The intensity of the connection between hematological parameters and value of NEFA and BHB decreases. Discussion: It is well known that dairy cows with excessive adiposity manifest a low-grade inflammation and that elevated NEFA concentrations present positive risk factors for many proinflammatory diseases. Analyzing the results in this examination, it has been noticed that the increase of NEFA and BHB after calving were significantly lower in group of cows treated with ketoprofen. Also, determined hematological parameters and values of NEFA and BHB are in correlation. The intensity of these connection decreases in ketoprofen treated cows. In current study, in cows treated with ketoprofen t...

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“…11,75 In a study in Israel, it was found that giving ketoprofen intramuscularly for 5 days allowed affected cows to return to 75% of their daily milk production recorded prior to their mastitis infection. 76 Upon initial diagnosis of clinical mastitis, the animals were given antimicrobials in combination with ketoprofen. A secondary portion of the study included ketoprofen treated versus a placebo-treated control group.…”

Section: Nsaid Therapy With Naturally Occurring Mastitismentioning

confidence: 99%