Anti-inflammatory effects of the products from Wilbrandia ebracteata on carrageenan-induced pleurisy in mice

Peters, Rodrigo Rebelo; Saleh, Twana; Lora, Maximilien; Patry, Christian; Brum‐Fernandes, Artur J. de; Farías, Mareni Rocha; Ribeiro‐do‐Valle, Rosa Maria

doi:10.1016/s0024-3205(99)00200-3

Cited by 42 publications

(22 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This fraction decreases cellular migration, exsudate formation and inhibits prostaglandin E2 release (Peters et al, 1999). The oral treatment of the animals with the dichloromethanic fraction produced a significant reduction of articular incapacitation; reduced nitrite release into the zymosaninflamed joints, selectively inhibit COX-2 activity observed in vitro experiments.…”

Section: Introductionmentioning

confidence: 85%

See 1 more Smart Citation

High performance liquid chromatography determination of cucurbitacins in the roots of Wilbrandia ebracteata Cogn.

Krepsky¹,

Cervelin²,

Porath³

et al. 2009

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO: "Determinação de cucurbitacinas em raízes de Wilbrandia ebracteata Cogn. por cromatografia líquida de alta eficiência". Raízes de Wilbrandia ebracteata Cogn. (Cucurbitaceae), tradicionalmente empregada no tratamento de doenças reumáticas, contém cucurbitacinas, sendo di-hidrocucurbitacina B a mais abundante, enquanto cucurbitacina B está presente em menor quantidade. Foi desenvolvido um método para determinação quantitativa destas cucurbitacinas. Os parâmetros selecionados foram: eluente isocrático acetonitrila/H 2 0 40:60, fluxo 1,2 mL/min. e detecção em 230 nm. Diversas formas de preparo da amostra foram testadas, sendo que extração com diclorometano sob refluxo forneceu o melhor resultado. O processo de validação incluiu: linearidade, exatidão, repetibilidade e precisão intermediária. A curva de calibração para a dihidrocucurbitacina B foi linear de 40.00 to 400 µg/mL, a recuperação foi 95,5±3.01%, a precisão intermediária, 1,64% e a repetibilidade variou entre 1,30 a 2,05%. A curva de calibração da cucurbitacina B foi linear de 4,00 to 240 µg/mL, a recuperação encontrada foi igual a 96,6±2.45%, a precisão intermediária, 2,29% e a repetibilidade variou entre 1,03 a 2,95%. Análise do mesmo espécime de W. ebracteata uma vez por ano de 2002 a 2005 revelou grande aumento no teor de cucurbitacina B após a raiz ter sido atacada por herbívoro. Unitermos:Wilbrandia ebracteata, Cucurbitaceae, cucurbitacina B, di-hidrocucurbitacina B, interação planta herbívoro, validação.ABSTRACT: Roots of Wilbrandia ebracteata Cogn., Cucurbitaceae, used in folk medicine for treatment of rheumatic disease, are rich in cucurbitacins. Dihydrocucurbitacin B is the most abundant cucurbitacin while cucurbitacin B is a minor component. A reverse-phase HPLC system was developed for simultaneous quantitative assay of these cucurbitacins in the roots. The optimised experimental conditions were acetonitrile/H 2 0 40:60, flow-rate 1.2 mL/min., detection at 230 nm and isocratic elution. A variety of sample preparation modes were tested and the extraction with dichloromethane under reflux gave better results. The validation process included linearity, accuracy, repeatability and intermediate precision. The calibration curve of dihydrocucurbitacin B was linear from 40.00 to 400 µg/mL, the recovery was 95.5±3.01%, the intermediate precision was found to be 1.64% and the repeatability varied between 1.30 to 2.05%. The calibration curve of cucurbitacin B was linear from 4.00 to 240 µg/mL, intermediate precision was found to be 2.29% and repeatability varied between 1.03 to 2.95%. Analysis of the same specimen of W. ebracteata every year from 2002 to 2005 revealed a great rise on the cucurbitacin B concentration after the root was attacked by an herbivore. Keywords:Wilbrandia ebracteata, Cucurbitaceae, cucurbitacin B, dihydrocucurbitacin B, plantherbivore interaction, accuracy.disease (Corrêa, 1984, Simões et al., 1986. The antiinflammatory activity of the dichloromethanic fraction has been demonstrated (Peters et al., 1997;Peters et al., 1999)...

show abstract

Section: Introductionmentioning

confidence: 85%

“…The antiinflammatory activity of the dichloromethanic fraction has been demonstrated (Peters et al, 1997;Peters et al, 1999), as well as the anti-ulcer activity of the leaves (González & DiStasi, 2002) and absence of antitubercular activity (Pavan et al, 2009). Phytochemical studies of the roots…”

mentioning

confidence: 99%

High performance liquid chromatography determination of cucurbitacins in the roots of Wilbrandia ebracteata Cogn.

Krepsky¹,

Cervelin²,

Porath³

et al. 2009

Rev. bras. farmacogn.

View full text Add to dashboard Cite

show abstract

“…10,11 Cu B, the major compound in cucurbitacin, has a broad range of biological activity, including anti-inflammatory, hepatocurative, and hepatoprotective effects. [12][13][14] GL is one of the main components extracted from Glycyrrhiza glabra L. and it possesses a variety of pharmacological effects, including anti-inflammatory, antiviral, and antioxidative activity. [15][16][17] Both have potent pharmacological activity, but suffer from low bioavailability following oral administration due to poor water solubility.…”

Section: Et Almentioning

confidence: 99%

Application of spray granulation for conversion of mixed phospholipid-bile salt micelles to dry powder form: influence of drug hydrophobicity on nanoparticle reagglomeration

Lv¹,

Shen³

et al. 2014

IJN

View full text Add to dashboard Cite

The aim of this study was to investigate the feasibility of using spray granulation as a drying method to convert phospholipid (PL)-sodium deoxycholate (SDC)-mixed micelles (MMs) containing a water-insoluble drug to a solid dosage form and to evaluate how drugs with significantly different physicochemical properties affect the spray granulation process and subsequent in vitro and in vivo processes. Cucurbitacin B (Cu B) and glycyrrhizin (GL) were used as the model drugs. After spray granulation, the dried Cu B-PL/SDC-MM powder was completely redispersible within 15 minutes in vitro. Meanwhile, the area under the curve during 24 hours (AUC 0–24 ) and peak serum concentration from the dried powder were significantly ( P <0.05) lower than the values from Cu B-PL/SDC-MMs in vivo. However, a better result was obtained for GL, ie, the drug was redispersed completely within 5 minutes in vitro. Further, absorption from the dried GL-PL/SDC-MM powder was increased to the same level as that for GL-PL/SDC-MMs in vivo compared with the control group. The difference in these results can be found in Cu B and GL. Cu B nanoparticles reagglomerated when released, resulting in slower redispersibility and less absorption compared with the original PL-SDC-MMs. However, no agglomeration or delay was observed for GL. A possible explanation is the difference in surface hydrophobicity between Cu B and GL. The results of this study not only show that spray granulation is an effective drying technique that can complement spray-drying and freeze-drying, but also confirm that the physicochemical properties of a drug have a significant influence on the in vitro and in vivo performance of the dried powder obtained after spray granulation.

show abstract

“…10) Injection of carrageenin was given between the seventh and eighth ribs under the right upper limbs through a gauge needle, with penetration restricted to 6 mm by carefully inserting it through a rubber disc. 11,12) Another injection of treatment drugs was given to animals 3 h after the inflammatory stimulus. Control animals received the irritant and an equal volume of sterile saline.…”

Section: Tentative Fingerprint-efficacy Study Of Houttuynia Cordata Imentioning

confidence: 99%

Tentative Fingerprint-Efficacy Study of Houttuynia cordata Injection in Quality Control of Traditional Chinese Medicine

Lü

Liang

Xian-jin

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

Anti-inflammatory effects of the products from Wilbrandia ebracteata on carrageenan-induced pleurisy in mice

Cited by 42 publications

References 15 publications

High performance liquid chromatography determination of cucurbitacins in the roots of Wilbrandia ebracteata Cogn.

High performance liquid chromatography determination of cucurbitacins in the roots of Wilbrandia ebracteata Cogn.

Application of spray granulation for conversion of mixed phospholipid-bile salt micelles to dry powder form: influence of drug hydrophobicity on nanoparticle reagglomeration

Tentative Fingerprint-Efficacy Study of Houttuynia cordata Injection in Quality Control of Traditional Chinese Medicine

Contact Info

Product

Resources

About