Anti-inflammatory effects of the ethyl acetate extract of Aquilaria crassna inhibits LPS-induced tumour necrosis factor-alpha production by attenuating P38 MAPK activation

Kumphune, Sarawut; Prompunt, Eakapot; Phaebuaw, Kanitha; Sriudwong, Piyanuch; Pankla, Rungnapa; Thongyoo, Panumart

doi:10.4103/0973-8258.82099

Cited by 20 publications

(13 citation statements)

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“…This ethnopharmacological evidence, together with the current trends in bioprospecting, have spurred the interest of the scientific community to investigate claims using modern tools. This is manifested in the surge of the number of scientific publications in recent years, particularly those describing the pharmacological actions of agarwood, including the antidiabetic Jiang and Tu, 2011;Pranakhon et al, 2015;Zulkiflie et al, 2013), anti-inflammatory (Chitre et al, 2007;Kumphune et al, 2011;Rahman et al, 2012;Sattayasai et al, 2012;Zhou et al, 2008), anti-cancer (Dahham et al, 2014;Dahham, 2015a;Gunasekera et al, 1981;Hashim et al, 2014a), anti-depressant (Okugawa et al, 1993;Takemoto et al, 2008), and anti-oxidant (Dahham et al, 2014;Han and Li, 2012;Huda et al, 2009;Kamonwannasit et al, 2013;Miniyar et al, 2008;Moosa, 2010;Nik Wil et al, 2014;Owen and Jones, 2002;Ray et al, 2014;Sattayasai et al, 2012;Tay et al, 2014) Trees, 1998)). In response to this situation, sustainable agarwood planting and management with artificial induction of agarwood resin formation have been implemented.…”

Section: Discussionmentioning

confidence: 98%

Aquilaria spp. (agarwood) as source of health beneficial compounds: A review of traditional use, phytochemistry and pharmacology

Hashim

Kerr

Abbas

et al. 2016

Journal of Ethnopharmacology

170

127

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Section: Discussionmentioning

confidence: 98%

Aquilaria spp. (agarwood) as source of health beneficial compounds: A review of traditional use, phytochemistry and pharmacology

Hashim

Kerr

Abbas

et al. 2016

Journal of Ethnopharmacology

170

127

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“…In this study, the cytotoxic and inhibitory effects of agarwood were observed when the extracts from both plant part (leaf and branch) from healthy and uninfected Aquilaria subintegra were tested against MCF-7 breast cancer cells. Most study conducted using agarwood did not state whether the sample originated from healthy or infected agarwood trees [14,16,19,22,[24][25][26]. Extracts from leaf and branch demonstrate profound reduction of cell growth in both Cell Attachment Assay (CAA) and Cell Viability Assay (CVA) as well as showing significant IC50 value estimate via the Sulforhodamine B (SRB) Assay.…”

Section: Discussionmentioning

confidence: 99%

“…In terms of anti-inflammatory properties, studies showed that (i) hexane extract of heartwood of Aquilaria agallocha (in vitro at 500 µg/mL) gave 78.5 % protection of human red blood cells in hypotonic solution while at 100mg/kg, it resulted in reduction of carrageenan-induced paw edema in rats [23], (ii) soxhlet-extracted (ethyl acetate) heartwood of Aquilaria crassna inhibited TNF-α gene expression when tested in vitro on human peripheral blood mononuclear cells (hPBMCs) [24], (iii) ethanolic extract of Aquilaria sinensis leaf caused 65 % writhing reduction and 32 % to 51 % inhibiton of paw edema when tested at 848 mg/kg [25] and (iv) Aquilaria agallocha heartwood possessed analgesic effects that reduced writhing while increasing the latency of tail flicking and paw licking in male albino mice [26].…”

mentioning

confidence: 99%

Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells

Abbas¹,

Hashim²,

Salleh³

2018

jrp

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Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu (Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional Chinese Medicine and other communities in the Asian region. While the resin is formed in infected trees, this present study reported the cytotoxicity and attachment inhibition effects of leaf and branch extracts from uninfected agarwood tree against breast cancer cells. Qualitative extraction screening process was first conducted to screen for suitable extraction solvents and parts of plant (leaf or branch). Then, the in vitro anti-cancer screening assays including cytotoxicity and attachment assays were conducted. Branch sample extracted using ethanol and distilled water resulted in higher yield and more potent cancer inhibiting effects as compared to other solvents. Crude extract obtained after drying process using ethanol as solvent yielded 9.47 % g/g (branch) and 13.2 % g/g (leaf); distilled water as solvent yielded 9.33 % g/g (branch) and 12.8 % g/g (leaf), respectively. However, branch extract exhibited more potent cancerinhibiting effects with IC50 of 23 µg/mL (ethanol) and 38 µg/mL (distilled water) as compared to leaf (no intersection points in the plot). To this end, it can be concluded that extracts from uninfected agarwood tree (Aquilaria subintegra) possesses cytotoxic and anti-attachment effects on MCF-7 breast cancer cells with ethanolic branch extract being the most promising. The screening and selection of extraction solvent and plant type are crucial steps towards cost-effective extraction and further bioprocessing of bioactive compounds from agarwood tree.

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“…crassna is a plant with diverse traditional medicinal properties. A number of studies have confirmed that the essential oil is an active component of A. crassna stem bark [23,24]. However, very little is known about the pharmacological properties of this plant.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Anti-Cancer and Anti-Angiogenic Activity of Essential Oils Extracts from Agarwood (Aquilaria crassna)

Dahham¹,

Tabana²,

Doblin³

et al. 2016

Med Aromat Plants

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MAP, an open access journal extracts from Aquilaria crassna stem bark against panel of cancer cell lines; (ii) to study the antiangiogenic effects of Aquilaria crassna most active extract. Materials and Methods Chemicals and reagents All cell cultures and their reagents were purchased from Gibco (USA). Phosphate buffered saline, trypsin, HIFBS, PS, fibrinogen, aprotinin, thrombin, suramin, aprotinin, 6-aminocaproic acid, L-glutamine, thrombin, and gentamicin were purchased from Sigma (Germany). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) were procured from Sigma-Aldrich (USA). Dimethyl sulfoxide (DMSO) was purchased from Fluka (USA). Cell lines and culture conditions The HUVEC cell line (catalogue number, C2517A), human colorectal carcinoma cell line HCT-116 (catalogue number CCL-247), human hormone sensitive invasive breast cancer cell line MCF-7 (catalogue number CRL-1469), Human pancreatic carcinoma cell line (PANC-1) catalogue number (HTB-14) and human prostate cancer cell line PC-3 (catalog number CRL-1435) were purchased from ScienCell (USA). HUVECs were maintained in ECM (ScienCell) supplemented with endothelial cell growth supplements (ECGS), 5% HIFBS, and 1% PS. HCT-116 cells and PANC-1 were maintained in RPMI, whereas MCF-7 and PC-3 cells were maintained in DMEM and F12K medium, respectively. The media were supplemented with 5% HIFBS and 1% PS. Cells were cultured in a humidified incubator at 37°C supplied by 5% CO 2. Cell culture work was conducted in sterile conditions using a Class II biosafety cabinet (ESCO, USA).

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Anti-inflammatory effects of the ethyl acetate extract of Aquilaria crassna inhibits LPS-induced tumour necrosis factor-alpha production by attenuating P38 MAPK activation

Cited by 20 publications

References 1 publication

Aquilaria spp. (agarwood) as source of health beneficial compounds: A review of traditional use, phytochemistry and pharmacology

Aquilaria spp. (agarwood) as source of health beneficial compounds: A review of traditional use, phytochemistry and pharmacology

Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells

In Vitro Anti-Cancer and Anti-Angiogenic Activity of Essential Oils Extracts from Agarwood (Aquilaria crassna)

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