Anti-inflammatory effects of the Aralia elata and Cirsium japonicum in Raw264.7 cells and in vivo colitis model in mice and dogs

Kim, Ryunhee; Islam, Md Sadikul; Yoo, Yeo-Jin; Shin, Ha-Young; Lee, Jehee; Cho, Jeong‐Hwi; Park, Yang-Gyu; Choi, Jin-Young; Tae, Hyun‐Jin; Park, Byung-Yong

doi:10.1016/j.biopha.2022.113186

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…37 The findings of this experiment are compatible and directly compared with those of a recent report, which, similar to our protocol, assessed AE against LPS-induced inflammation in RAW 264.7 cells and DSS-induced colitis in mice, in which oxidative stress, TNFa, IL-6, IL-1b, and NO production were substantially reduced. 40 Moreover, we confirmed that a single plant extract of AE extracts, not a combined treatment of Cirsium japonicum and AE extracts, also improved the colitis animal models. As a result, the mechanism of the AE extract in the treatment of UC is based on its suppression of the inflammatory cytokines TNF-α, IL-6, and IL-1β and hindering NO production in the cells.…”

Section: Discussionsupporting

confidence: 59%

Anti-Inflammatory Effects ofAralia elataExtract Against Dextran Sodium Sulfate-Induced Colitis in Mice and Raw 264.7 Macrophage Cells Exposed to Lipopolysaccharide: First Report

Lee

Adam

et al. 2022

Natural Product Communications

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Aralia elata (AE) is an anti-inflammatory, polyphenolic containing medicinal plant. However, little is known about AE and its application to ulcerative colitis (UC). This study aimed to confirm AE extract's antioxidant and anti-inflammatory effects in vivo and in vitro. The in vitro antioxidant activity was evaluated by measuring total polyphenol and flavonoid content in AE extract. AE extract (10 000 mg/L) contained 186.8 mg GAE/g polyphenol and 81.9 mg QE/g flavonoid. Mice were divided into 6 groups, including control, which received normal saline, and treatment groups, which received dextran sodium sulfate (DSS) with or without AE extract (250, 500, and 1000 mg/kg). RAW 264.7 macrophage cells were divided into 2 groups: control and treatment. RAW 264.7 macrophage cells treated with sterile double distilled water, 1 mg/L lipopolysaccharide (LPS), and AE extracts (25, 50, 75, 100 µg/mL) were used to assess the cytotoxicity and anti-inflammatory activity. High-performance liquid chromatography, enzyme-linked immunosorbent assay (ELISA) kits, and histology were employed to analyze the AE extract contents, nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as oxidative stress markers. In addition, the disease activity index (DAI) and cytotoxicity were determined in mice and cells, respectively. High-performance liquid chromatography analysis revealed that AE extract is rich in chlorogenic acid (96 ± 0.01 mg/g). DSS increased the DAI and levels of TNF-α, IL-1β, and immune cell infiltration compared with those of the control animals. Furthermore, LPS eventually reduced cell viability and increased the levels of NO, TNF-α, IL-1β, and IL-6 in contrast to control cells. After treatment, a noticeable reduction was observed in the levels of DAI, NO, TNF-α, IL-1β, and IL-6 compared to those without AE treatments. Overall, AE extract is safe and had anti-inflammatory properties. Therefore, AE extract can be considered a potential pre-treatment supplement for UC.

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Section: Discussionsupporting

confidence: 59%

Anti-Inflammatory Effects ofAralia elataExtract Against Dextran Sodium Sulfate-Induced Colitis in Mice and Raw 264.7 Macrophage Cells Exposed to Lipopolysaccharide: First Report

Lee

Adam

et al. 2022

Natural Product Communications

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“…All the mice in the 3 groups were fed until the end of the 12th week. After finishing the experimental periods, mice were euthanized by intraperitoneal injection of urethan (Sigma-Aldrich, St. Louis, MO, USA, 700 mg/kg), and blood, spleen, and kidney samples were collected [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Inulin Reduces Kidney Damage in Type 2 Diabetic Mice by Decreasing Inflammation and Serum Metabolomics

He,

Li,

Yan

et al. 2024

Journal of Diabetes Research

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This study is aimed at assessing the impact of soluble dietary fiber inulin on the treatment of diabetes-related chronic inflammation and kidney injury in mice with type 2 diabetes (T2DM). The T2DM model was created by feeding the Institute of Cancer Research (ICR) mice a high-fat diet and intraperitoneally injecting them with streptozotocin (50 mg/kg for 5 consecutive days). The thirty-six ICR mice were divided into three dietary groups: the normal control (NC) group, the T2DM (DM) group, and the DM + inulin diet (INU) group. The INU group mice were given inulin at the dose of 500 mg/kg gavage daily until the end of the 12th week. After 12 weeks, the administration of inulin resulted in decreased serum levels of fasting blood glucose (FBG), low-density lipoprotein cholesterol (LDL-C), blood urea nitrogen (BUN), and creatinine (CRE). The administration of inulin not only ameliorated renal injury but also resulted in a reduction in the mRNA expressions of inflammatory factors in the spleen and serum oxidative stress levels, when compared to the DM group. Additionally, inulin treatment in mice with a T2DM model led to a significant increase in the concentrations of three primary short-chain fatty acids (SCFAs) (acetic acid, propionic acid, and butyric acid), while the concentration of advanced glycation end products (AGEs), a prominent inflammatory factor in diabetes, exhibited a significant decrease. The results of untargeted metabolomics indicate that inulin has the potential to alleviate inflammatory response and kidney damage in diabetic mice. This beneficial effect is attributed to its impact on various metabolic pathways, including glycerophospholipid metabolism, taurine and hypotaurine metabolism, arginine biosynthesis, and tryptophan metabolism. Consequently, oral inulin emerges as a promising treatment option for diabetes and kidney injury.

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“…Histopathological changes in the brain was observed through hematoxylin and eosin (HE) staining following the protocol previously described ( Kim et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Antiviral activity of prion protein against Japanese encephalitis virus infection in vitro and in vivo

Hong,

Munna,

Moon

et al. 2023

Virus Research

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Anti-inflammatory effects of the Aralia elata and Cirsium japonicum in Raw264.7 cells and in vivo colitis model in mice and dogs

Cited by 9 publications

References 37 publications

Anti-Inflammatory Effects ofAralia elataExtract Against Dextran Sodium Sulfate-Induced Colitis in Mice and Raw 264.7 Macrophage Cells Exposed to Lipopolysaccharide: First Report

Anti-Inflammatory Effects ofAralia elataExtract Against Dextran Sodium Sulfate-Induced Colitis in Mice and Raw 264.7 Macrophage Cells Exposed to Lipopolysaccharide: First Report

Inulin Reduces Kidney Damage in Type 2 Diabetic Mice by Decreasing Inflammation and Serum Metabolomics

Antiviral activity of prion protein against Japanese encephalitis virus infection in vitro and in vivo

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