Anti‐inflammatory effects of sevoflurane and mild hypothermia in endotoxemic rats

Hofstetter, Christian; Boost, Kim Alexander; Flondor, Michael; Basagan-Mogol, Elif; Betz, Christoph; Homann, M; Mühl, Heiko; Pfeilschifter, Joseph; Zwißler, Bernhard

doi:10.1111/j.1399-6576.2007.01353.x

Cited by 67 publications

(43 citation statements)

References 26 publications

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“…That this effect has clinical relevance is suggested by the following lines of evidence. First, this anti-inflammatory effect of the volatile anesthetics sevoflurane, isoflurane and desflurane was also demonstrated in animal models of inflammation (6,18,34) and with human cells from healthy donors (35). Second, the inhibitory properties on the IκB· degradation and inducing properties on IκB· protein content occurred at concentrations comparable to those used during sevoflurane or isoflurane inhalation in clinical practice (36).…”

Section: Discussionmentioning

confidence: 99%

Sevoflurane and isoflurane decrease TNF-α-induced gene expression in human monocytic THP-1 cells: Potential role of intracellular IκBα regulation

Boost

Leipold²,

Scheiermann³

et al. 2009

Int J Mol Med

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Abstract. The nuclear factor (NF)-κB/inhibitory (I)κB· pathway is one of the most important intracellular signal transduction pathways during inflammation which is induced by a variety of major early response cytokines. Recent studies suggest that volatile anesthetics interfere with inflammatory cytokine production through inhibition of intracellular signal transduction pathways. We, therefore, aimed to investigate the effects of the volatile anesthetics sevoflurane and isoflurane on NF-κB/IκB·-dependent intracellular signal transduction in human monocytic THP-1 cells induced by tumor necrosis factor-· (TNF-·) and production of interleukin-8 (IL-8) and downstream heme oxygenase-1 (HO-1). THP-1 cells, a human monocytic cell line, were used in an in vitro model which enables the exposure to volatile anesthetics. Using this model, THP-1 cells were subjected to sevoflurane or isoflurane exposure (1 MAC each) and were stimulated with TNF-· (50 or 100 ng/ml). Compared to untreated cells, expression of intracellular HO-1-protein and release of IL-8 into cell culture supernatants and corresponding mRNA expression were attenuated in THP-1 cells exposed to sevoflurane and isoflurane, respectively. Moreover, translocation of NF-κB and degradation of IκB· were markedly reduced by both anesthetics. Notably, under unstimulated conditions, exposure to sevoflurane induced a sustained upregulation of the IκB· content in THP-1 cells. We demonstrated inhibition of TNF-·-induced gene expression and release of IL-8 and HO-1 in human monocytic THP-1 cells exposed to both volatile anesthetics. This was associated with an upregulated intracellular IκB· content followed by decreased NF-κB translocation. This was more sustained during exposure to sevoflurane and may provide an additional intracellular mechanism for the anti-inflammatory effects associated with sevoflurane administration.

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Section: Discussionmentioning

confidence: 99%

Sevoflurane and isoflurane decrease TNF-α-induced gene expression in human monocytic THP-1 cells: Potential role of intracellular IκBα regulation

Boost

Leipold²,

Scheiermann³

et al. 2009

Int J Mol Med

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“…Moreover, cold inhibits the expression of inflammatory mediators; hypothermia inhibits activation of neutrophils and expression of intercellular adhesion molecule-1 (ICAM-1), the adhesion and activation of neutrophils during inflammation (Hanusch et al, 2007). Therefore hypothermia induced expression of the anti-inflammatory cytokines IL-10 (Scumpia et al, 2004); hypothermia attenuates the inflammatory response during WBC, thus contributing to its beneficial role in organ protection (Hofstetter et al, 2007). As elevated serum CK is a characteristic marker of exertional rhabdomyolysis, it may be used for measuring the effects of workload, recovery and possible overtraining.…”

Section: Article In Pressmentioning

confidence: 99%

Effects of whole-body cryotherapy on serum mediators of inflammation and serum muscle enzymes in athletes

Banfi

Melegati

Barassi

et al. 2009

Journal of Thermal Biology

145

132

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a b s t r a c tWhole-body cryotherapy (WBC) covers a wide range of therapeutic applications and consists of briefly exposing the body to extremely cold air. In sports medicine, WBC is used to improve recovery from muscle injury; however, empirical studies on its application to this area are lacking. To fill this gap, we compared changes in immunological parameters (C3, IgA, IgM, IgG, C-reactive protein, PGE2), cytokines (IL-2, IL-8, IL-10), adhesion molecules (sICAM-1), and muscle enzymes (creatine kinase [CK], lactate dehydrogenase [LAD]) before and after WBC in 10 top-level Italian National team rugby players. The subjects underwent five sessions on alternate days once daily for 1 week. During the study period, the training workload was the same as that of the previous weeks. Compared to baseline values, immunological parameters remained unchanged, while CK and LAD levels significantly decreased after treatment. No alterations in immunological function were observed but there is a decrease in proinflammatory cytokine/chemokine and an increase in anti-inflammatory cytokine.As measured by changes in serum CK and LAD concentrations, and cytokines pathway, short-term cold air exposure was found to improve recovery from exercise-induced muscle injury and/or damage associated with intense physical training.

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“…By contrast, postconditioning would be more applicable in a clinical situation. Hofstetter et al (21) examined the postconditioning effect of sevoflurane in an in vivo model of LPS-induced endotoxemia in rats, where administration of sevoflurane 15 min after intravenous injection of LPS resulted in decreased TNF-α and IL-1β. The present study demonstrated that postconditioning is effective even 2 h after LPS stimulation and indicates anesthetic postconditioning could be initiated in a later phase of clinical treatment.…”

Section: A B Cmentioning

confidence: 99%

Volatile anesthetic sevoflurane ameliorates endotoxin-induced acute lung injury via microRNA modulation in rats

et al. 2015

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Abstract. Volatile anesthetics have a lung protective effect in acute lung injury (ALI). Our previous study showed sevoflurane affects the expression of microRNA (miRNA) that control various physiological systems by regulating messenger RNA (mRNA) expression. However, the association between the anti-inflammatory effect of sevoflurane and miRNAs modulation remains unknown. The aim of the present study was to investigate the effect of sevoflurane and the expression of miRNAs in an endotoxin-induced ALI model in rats. Wistar rats were randomly assigned to three groups [lipopolysaccharide (LPS), LPS-sevoflurane and control; n=8/group]. All the rats were mechanically ventilated and intravenously-administered LPS (saline as control). Two hours post-injury, general anaesthesia was performed for 4 h with 2% sevoflurane (LPS-sevoflurane). The LPS and the control groups did not receive anaesthesia. The severity of ALI was evaluated by partial pressure of oxygen/fraction of inspired oxygen and the mRNA expression of inflammatory cytokine. The miRNA expression in lung tissue was analyzed by a reverse transcription-quantitative polymerase chain reaction. LPS caused ALI, evidenced by the impairment of pulmonary function and increased mRNA levels of tumor necrosis factor-α, interleukin-6 and nuclear factor-κB. Sevoflurane improved pulmonary function and inhibited the increased mRNAs. Of the 219 miRNAs detected, 15 and nine miRNAs were significantly changed in the LPS and LPS-sevoflurane group, respectively. In the LPS-sevoflurane group, the expression of several miRNAs that regulate inflammation was significantly changed compared to the LPS group. In conclusion, the present data showed that sevoflurane influences the expression of the miRNAs that regulate inflammation. This result suggests that the changes in miRNA expression are involved in the lung protective mechanisms of volatile anesthetics. IntroductionAcute lung injury (ALI) is a major complication in critically ill patients (1). Therefore, it is important to preserve lung function and prevent further exacerbation for those patients whose lungs are already vulnerable prior to surgery. The volatile anesthetic sevoflurane is known to have organ protective effects in several pathological conditions. For example, Voigtsberger et al (2) have recently shown that sevoflurane ameliorates gas exchange and attenuates lung damage in a model of lipopolysaccharide (LPS)-induced ALI.MicroRNA (miRNA) is a newly discovered and non-coding RNA that regulates genome expression at the post-transcriptional level (3). miRNA is estimated to control 30-90% of human genes (4). miRNA has been suggested to either suppress messenger RNA (mRNA) translation or reduce mRNA stability by binding particular regions of mRNA (5). Recent published data have also revealed that the expression and function of miRNA are associated with a broad range of diseases.In our previous study, it was found that sevoflurane affects the expression of miRNAs in normal rat lung (6). However, such effects of anesthetic ALI ...

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Anti‐inflammatory effects of sevoflurane and mild hypothermia in endotoxemic rats

Abstract: Our data suggest that sevoflurane and mild hypothermia attenuate the inflammatory response during endotoxemia in vivo thus contributing to their beneficial role in clinical organ protection.

Cited by 67 publications

References 26 publications

Sevoflurane and isoflurane decrease TNF-α-induced gene expression in human monocytic THP-1 cells: Potential role of intracellular IκBα regulation

Sevoflurane and isoflurane decrease TNF-α-induced gene expression in human monocytic THP-1 cells: Potential role of intracellular IκBα regulation

Effects of whole-body cryotherapy on serum mediators of inflammation and serum muscle enzymes in athletes

Volatile anesthetic sevoflurane ameliorates endotoxin-induced acute lung injury via microRNA modulation in rats

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