Anti-inflammatory effects of ozonated water in an experimental mouse model

Abstract

Abstract. Previous studies have suggested that ozonated water is safe and possesses antibacterial effects for treatment of experimental peritonitis rats. In this study, we evaluated the anti-inflammatory effects of ozonated water that was intraperitoneally injected into an experimental inflammatory mouse model. The concentrations of dissolved ozone decreased constantly and lineally, while the half-life of dissolved ozone was 36.8±2.7 min (27˚C). The 10-ppm ozonated water was injected intraperitoneally into mice with lipopolysaccharide (LPS)-induced acute inflammation. The results showed that the intraperitoneal injection of ozonated water decreased the levels of tumor necrosis factor-α (TNF-α) and increased the activity of superoxide dismutase (SOD). The results suggest that ozonated water has anti-inflammatory properties and is a potential therapeutic option for acute inflammation.

The efficacy of ozone therapy in neonatal rats with hypoxic ischemic brain injury

“…Ozone (O 3 ) is a cyclic gas composed of 3 oxygen atoms (4). Recently, medical ozone therapy has been shown to be a safe, economic, and effective treatment for many conditions, including advanced ischemic lesions, peritonitis, infected wounds, chronic skin ulcers, initial gangrene, and burns (5).…”

Persistence of Healthcare-Associated (Nosocomial) Infections Due to Inadequate Hand Hygiene: Part 3—Application of Human Factors Engineering to an Ozone Hand Sanitizer

“…Solubilized ozone has been used safely in several animal models and human clinical studies, with no reported side effects [5]. But until now it has been difficult to reliably control the concentration of ozone in water [6]. We describe an automated device designed to improve hand-hygiene using ozonated water and efficacy of the approach in test systems.…”

Effects of Prophylactic Ozone Therapy on General Anesthesia and Surgical Stress Response: Neutrophil/Lymphocyte Ratio and Ischemia-Modified Albumin

“…Conclusions:When General anesthesia and surgical stress response was evaluated using inflammatory parameters of both NLR and IMA, there was significantly less of an increase in levels in rabbits given ozone compared to the control group. Ozone therapy comprises a medical O3/O2 gas mix applied through a variety of pathways [5]. Ozone therapy has known antioxidant, antibacterial, immunomodulator, analgesic, anti-inflammatory, and many other beneficial effects [5,6].…”

“…Ozone therapy comprises a medical O3/O2 gas mix applied through a variety of pathways [5]. Ozone therapy has known antioxidant, antibacterial, immunomodulator, analgesic, anti-inflammatory, and many other beneficial effects [5,6]. Toxic if taken at high concentrations through inhalation pathways, ozone undertakes a trigger role in releasing inflammatory mediators [7].…”

“…Toxic if taken at high concentrations through inhalation pathways, ozone undertakes a trigger role in releasing inflammatory mediators [7]. When ozone therapy is administered, apart from by inhalation, it reduces the release of antioxidants like superoxide dismutase (SOD), as well as proinflammatory cytokines like IL-1, IL-6 and TNF-α, showing an anti-inflammatory effect [5,8].…”