Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice

Abstract

Considerable literature has been published on polysaccharides, which play a critical role in regulating the pathogenesis of inflammation and immunity. In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated. The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway. For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum. Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora. Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon. These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.

Structural Analysis and Anti-Inflammatory Effect of a Digalactosyldiacylglycerol-Monoestolide, a Characteristic Glycolipid in Oats

“…These results demonstrated that DGDG-LAHLA suppressed the production of both pro-inflammatory and anti-inflammatory cytokines. Such simultaneous suppression of both pro-inflammatory and anti-inflammatory cytokines has been reported for several other compounds (e.g., tribulusamide D [ 21 ] and Mytilus coruscus polysaccharide [ 22 ]). In these studies, the inhibition of an upstream transcription factor, NF-κB, was suggested as an underlying mechanism.…”

Recent Discoveries on Marine Organism Immunomodulatory Activities

“…In mice with dextran sodium sulphate-induced ulcerative colitis (DSS) after treatment with MP, the integrity of the intestinal barrier and the Firmicutes/Bacteroidetes ratio were improved; there was also a greater abundance of some probiotics, such as Anaerotruncus , Lactobacillus, Desulfovibrio , Alistipe, Odoribacter , and Enterorhabdus , in the colon. Thus, MP could be a promising dietary candidate for treating ulcerative colitis and improving immunity [ 109 ].…”

Effect of mussel polysaccharide on glucolipid metabolism and intestinal flora in type 2 diabetic mice

“…Currently, several marine polysaccharides show potential in alleviating insulin resistance. In our previous study, we reported the potential of active polysaccharides in mussels in alleviating inflammation and regulating intestinal flora 21‐23 . Based on those findings, we used T2D as the research object and continued to explore its impact on glucose and lipid metabolism disorders and microbiota changes.…”