Anti-inflammatory effects of millet bran derived-bound polyphenols in LPS-induced HT-29 cell via ROS/miR-149/Akt/NF-κB signaling pathway

Shi, Jiangying; Shan, Shuhua; Li, Hanqing; Song, Guisheng; Li, Zhuoyu

doi:10.18632/oncotarget.20216

Cited by 58 publications

(37 citation statements)

References 47 publications

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“…MicroRNA-21a, miR-146b, and miR-149 were highlighted as those miRNAs with the most prominent increase upon 3R4F CS versus sham in lung compared with the other tissues. For all 3 miRNAs, an association with immune-responses have been reported: miR-21a is a negative immune regulator in mouse liver regeneration (via nuclear factor kappa B [NF-κB] inhibition) ( Marquez et al 2010 ) and of the macrophage response in peritonitis ( Barnett et al 2016 ), miR-146b is an innate immune cell-associated miRNA ( Hou et al 2009 ), and miR-149 has been implicated in immune-regulatory feedback loops ( Shi et al 2017 ; Xu et al 2014 ). Overall, this is consistent with the prominent immune response observed in the lung upon 3R4F CS exposure.…”

Section: Discussionmentioning

confidence: 99%

Respiratory Effects of Exposure to Aerosol From the Candidate Modified-Risk Tobacco Product THS 2.2 in an 18-Month Systems Toxicology Study With A/J Mice

Titz

Sewer

Luettich

et al. 2020

Toxicological Sciences

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Smoking cessation is the most effective measure for reducing the risk of smoking-related diseases. However, switching to less harmful products (modified risk tobacco products [MRTP]) can be an alternative to help reduce the risk for adult smokers who would otherwise continue to smoke. In an 18-month chronic carcinogenicity/toxicity study in A/J mice (OECD Test Guideline 453), we assessed the aerosol of Tobacco Heating System 2.2 (THS 2.2), a candidate MRTP based on the heat-not-burn principle, compared with 3R4F cigarette smoke (CS). To capture toxicity- and disease-relevant mechanisms, we complemented standard toxicology endpoints with in-depth systems toxicology analyses. In this part of our publication series, we report on integrative assessment of the apical and molecular exposure effects on the respiratory tract (nose, larynx, and lungs). Across the respiratory tract, we found changes in inflammatory response following 3R4F CS exposure (e.g., antimicrobial peptide response in the nose), with both shared and distinct oxidative and xenobiotic responses. Compared with 3R4F CS, THS 2.2 aerosol exerted far fewer effects on respiratory tract histology, including adaptive tissue changes in nasal and laryngeal epithelium and inflammation and emphysematous changes in the lungs. Integrative analysis of molecular changes confirmed the substantially lower impact of THS 2.2 aerosol than 3R4F CS on toxicologically and disease-relevant molecular processes such as inflammation, oxidative stress responses, and xenobiotic metabolism. In summary, this work exemplifies how apical and molecular endpoints can be combined effectively for toxicology assessment and further supports findings on the reduced respiratory health risks of THS 2.2 aerosol.

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Section: Discussionmentioning

confidence: 99%

Respiratory Effects of Exposure to Aerosol From the Candidate Modified-Risk Tobacco Product THS 2.2 in an 18-Month Systems Toxicology Study With A/J Mice

Titz

Sewer

Luettich

et al. 2020

Toxicological Sciences

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“…TNF- α , IL-6, IL-1 β , and PGE2 expression in the cultured cell supernatants was quantified using an enzyme-linked immunosorbent assay (ELISA) kit (Quantikine® ELISA, R&D Systems, MN, USA) [ 18 ] according to the manufacturer's instructions. Absorbance was measured at 450 nm using a FlexStation 3 microplate reader (Molecular Devices, USA).…”

Section: Methodsmentioning

confidence: 99%

Pinus thunbergii Parl. Extracts Reduce Acute Inflammation by Targeting Oxidative Stress

Yoon

Choi

Kang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Pinus thunbergii Parl. (PTP) has traditionally been used for edible and medicinal purposes to treat several disorders, including diabetes and neuralgia. Therefore, this study sought to evaluate the inhibitory effects of PTP leaf ethanol extracts on acute inflammation. Moreover, the reactive oxygen species (ROS) scavenging activity, superoxide dismutase (SOD) activity, lipopolysaccharide (LPS)-induced nitric oxide (NO) generation, and H2O2-induced lipid peroxidation capacity of PTP were assessed in vitro in RAW 264.7 macrophages. Our results suggest that PTP prevents cell damage caused by oxidative free radicals and downregulates the expression of LPS-induced inflammation-associated factors including inducible nitric oxidase synthetase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). PTP inhibited NO production by 53.5% ( P < 0.05 ) and iNOS expression by 71.5% ( P < 0.01 ) at 100 µg/mL. PTP at 100 µg/mL also inhibited ROS generation by 58.2% ( P < 0.01 ) and SOD activity by 29.3%, as well as COX-2 expression by 83.3% ( P < 0.01 ) and PGE2 expression by 98.6% ( P < 0.01 ). The anti-inflammatory effects of PTP were confirmed in vivo using an arachidonic acid (AA)-induced ear edema mouse model. Ear thickness and myeloperoxidase (MPO) activity were evaluated as indicators of inflammation. PTP inhibited edema formation by 64.5% ( P < 0.05 ) at 1.0 mg/ear. A total of 16 metabolites were identified in PTP extracts and categorized into subgroups, including two phenolic acids (mainly quinic acid), seven flavonoids, five lignans, one sesquiterpenoid, and one long-chain fatty acid. Therefore, our results suggest that PTP possesses anti-inflammatory properties.

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“… 9 An animal experiment showed that foxtail millet feeding improved insulin sensitivity and cholesterol metabolism in genetically type 2 diabetic mice. 10 A recent study indicated that bound polyphenols of inner shell from foxtail millet bran can display anti-inflammatory effects in LPS-induced HT-29 cells and in nude mice, 11 another clinical trial have shown the blood glucose lowering of foxtail millet. 12 …”

Section: Introductionmentioning

confidence: 99%

The gastroprotective effect of the foxtail millet and adlay processing product against stress-induced gastric mucosal lesions in rats

Lin

Sheu

Sheen

et al. 2020

Journal of Traditional and Complementary Medicine

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Foxtail millet ( Setaria italica (L.) P. Beauv.) and adlay ( Coix lachryma-jobi L. var. ma-yuen Stapf.) seeds have substantial benefits possesses remarkable edible and nutritive values, and ease of processing and food manufacturing. They have nutraceutical properties in the form of antioxidants which prevent deterioration of human health and have long been used in traditional Chinese medicine as a remedy for many diseases. The present study is designed to investigate the gastroprotective effect of foxtail millet and adlay processing product (APP) diet on water immersion restraint stress (WIRS) induced ulceration in rats. We examined the effects of intake of AIN-93G diet containing either foxtail millet (10, 20 and 40%, 4 weeks) or APP (15 and 30%, 5 weeks) on macroscopic ulcer index (UI), plasma calcium level, lipid peroxidation products (estimated by the thiobarbituric acid reactive substances; TBARS), non-protein sulfhydryl (NPSH), digestive enzyme activities, and histopathology were determined. The results showed that pretreatment with millet and adlay diets significantly prevented the gastric mucosal lesion development. In addition, ulcerated rats showed depletion of NPSH levels whereas treatment with millet and adlay reverted this decline in stress-induced rats. Histological studies confirmed the results. The finding suggests that millet and adlay diets promote ulcer protection by the decrease in ulcer index, TBARS values and increase NPSH concentrations. Millet and adlay diets retain the advantage of being a natural product which may protect the gastric mucosa against ulceration.

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Anti-inflammatory effects of millet bran derived-bound polyphenols in LPS-induced HT-29 cell via ROS/miR-149/Akt/NF-κB signaling pathway

Cited by 58 publications

References 47 publications

Respiratory Effects of Exposure to Aerosol From the Candidate Modified-Risk Tobacco Product THS 2.2 in an 18-Month Systems Toxicology Study With A/J Mice

Respiratory Effects of Exposure to Aerosol From the Candidate Modified-Risk Tobacco Product THS 2.2 in an 18-Month Systems Toxicology Study With A/J Mice

Pinus thunbergii Parl. Extracts Reduce Acute Inflammation by Targeting Oxidative Stress

The gastroprotective effect of the foxtail millet and adlay processing product against stress-induced gastric mucosal lesions in rats

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