Anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium on LPS-stimulated Raw264.7 cells

Kwon, Ok‐Kyoung; Lee, Mee-Young; Yuk, Ji-Eun; Oh, Sei-Ryang; Chin, Young‐Won; Lee, Hyeong‐Kyu; Ahn, Ki Hoon

doi:10.1016/j.jep.2010.04.002

Cited by 82 publications

(53 citation statements)

References 24 publications

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“…A number of studies have suggested that MAPKsignaling pathways are considerable targets for controlling inflammation since JNK, p38 and ERK are activated by LPS; they also induce cytokine production (Dong et al, 1993;Kwon et al, 2010;Uto et al, 2010;Yu et al, 2011). In addition, MAPKs have been reported to mediate LPS-induced iNOS and KF-kB activations (Lu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Boehmeria niveaattenuates LPS-induced inflammatory markers by inhibiting p38 and JNK phosphorylations in RAW264.7 macrophages

Sung

Davaatseren

Kim

et al. 2013

Pharmaceutical Biology

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Context: Boehmeria nivea (Linn.) Gaudich (Urticaceae), a natural herb, has a long history of treating several diseases including wound healing. However, the anti-inflammatory effect of B. nivea has not been investigated. Objective: We investigated whether the 70% ethanol extract of B. nivea (Ebn) can exert antiinflammatory activity. Several phenolic compounds of extracts were determined to provide further information on the correlation between anti-inflammatory effects and phenolic compounds. Materials and methods: We prepared a 70% ethanol extract of B. nivea leaves and evaluated its anti-inflammatory activity (200, 400, 800, 1200 mg/mL) by measuring the secretions of nitric oxide (NO), tumor necrosis factor alpha (TNF-a) and interleukin 6 (IL-6), which were stimulated by lipopolysaccharide (LPS) in RAW264.7 macrophages. The total phenolic compounds were determined by the Folin-Ciocalteu method and major compounds were determined by HPLC. Results: Ebn was able to abolish the LPS-induced secretions of NO, TNF-a and IL-6. It also decreased the protein levels (IC 50 ¼ 186 mg/mL) of LPS-induced inducible nitric oxide synthase (iNOS). The LPS stimulated p38, JNK and ERK phosphorylations significantly more than the controls. Surprisingly, although Ebn reduced p38 and JNK phosphorylations, it did not influence ERK phosphorylation. We found that Ebn revealed several major compounds such as chlorogenic acid (1.96 mg/100 g), rutin (46.48 mg/100 g), luteolin-7-glucoside (11.29 mg/100 g), naringin (1.13 mg/100 g), hesperidin (23.69 mg/100 g) and tangeretin (1.59 mg/100 g). Discussion and conclusion: Boehmeria nivea exerts an anti-inflammatory effect on macrophages by inhibiting p38 and JNK, suggesting that it may be used as a functional ingredient against inflammation.

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Section: Discussionmentioning

confidence: 99%

“…Lindl., and Lilium lancifolium Thunb. (Liliaceae) exert anti-inflammatory effects via the inhibition of JNK, p38 and ERK (Kwon et al, 2010;Uto et al, 2010;Yu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Boehmeria niveaattenuates LPS-induced inflammatory markers by inhibiting p38 and JNK phosphorylations in RAW264.7 macrophages

Sung

Davaatseren

Kim

et al. 2013

Pharmaceutical Biology

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“…5 and 6), based on the knowledge that COX-2 expression coincides with PGE 2 secretion in the pathogenesis of various inflammatory diseases. Several groups have recently demonstrated that the effects of plant extracts on the expression of inflammation-related genes [19][20][21] are strongly related to inhibitory effects on COX-2 protein expression in LPS-stimulated macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…Induction of COX-2 has been shown to require activation of the ERK signaling pathway [24], while other researchers have demonstrated that constitutively active MEKK1 induces COX-2 expression by activating the SEK1/MKK4-p38 kinase pathway [25]. The c-Jun N-terminal kinases (JNKs), known as stress-activated protein kinases (SAPKs), are also associated with induction of COX-2 [19]. Here, we have shown that EPE inhibits LPS-induced COX-2 expression via suppressing ERK, p38, and JNK phosphorylation (Fig.…”

Section: Discussionmentioning

confidence: 99%

Ethanol Extract of Elaeocarpus petiolatus Inhibits Lipopolysaccharide-Induced Inflammation in Macrophage Cells

et al. 2011

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Elaeocarpus petiolatus is known to exert active oxygen scavenging, anti-aging, and whitening actions. However, the biological effects of E. petiolatus on inflammation and the underlying mechanisms are yet to be established. In the present study, we investigated the anti-inflammatory effects of the ethanol extract from E. petiolatus (EPE) bark in murine Raw264.7 macrophages stimulated with lipopolysaccharide (LPS). EPE inhibited the production of PGE(2), TNF-α, and IL-1β in a dose-dependent manner in Raw264.7 cells stimulated with LPS. The decrease in PGE(2) production was correlated with reduced COX-2 expression. Furthermore, EPE suppressed the phosphorylation of extracellular signal-related kinases (ERK), c-Jun N-terminal kinase (JNK), and p38 as well as translocation of the NF-κB p65 subunit from the cytosol to nucleus. Our results suggest that EPE exerts anti-inflammatory activity through inhibition of inflammatory mediators, such as PGE(2), TNF-α, and IL-1β, and downregulation of COX-2 via suppression of NF-κB translocation and phosphorylation of ERK, JNK, and p38 in LPS-stimulated Raw264.7 cells.

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“…They also found that the iNOS and COX-2 expression was suppressed simultaneously. The mechanism-based study in this paper indicates that anti-inflammatory effects of methanol extract from L. lancifolium Thunb root are due to down-regulation of iNOS and COX-2 via suppression of NF-B activation and nuclear translocation as well as blocking of ERK and JNK signaling in LPSstimulated Raw264.7 cells [14]. Water extract of L. lancifolium Thunb root was found to significantly inhibit the number of macrophages and neutrophils in BALF due to pulmonary inflammatory response in a CS-exposed mouse.…”

Section: Pharmacological Studiesmentioning

confidence: 80%

Lilium lancifolium 百合 (Baihe, Tiger Lily)

Liu

2015

Dietary Chinese Herbs

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Anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium on LPS-stimulated Raw264.7 cells

Cited by 82 publications

References 24 publications

Boehmeria niveaattenuates LPS-induced inflammatory markers by inhibiting p38 and JNK phosphorylations in RAW264.7 macrophages

Boehmeria niveaattenuates LPS-induced inflammatory markers by inhibiting p38 and JNK phosphorylations in RAW264.7 macrophages

Ethanol Extract of Elaeocarpus petiolatus Inhibits Lipopolysaccharide-Induced Inflammation in Macrophage Cells

Lilium lancifolium 百合 (Baihe, Tiger Lily)

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