Anti-inflammatory effects of Huangqin tang extract in mice on ulcerative colitis

Chen, Peidong; Zhou, Xi Kathy; Zhang, Li; Shan, Mingqiu; Bao, Beihua; Cao, Yudan; Kang, An Na; Ding, Anwei

doi:10.1016/j.jep.2014.12.039

Cited by 53 publications

(32 citation statements)

References 38 publications

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“…Treatment with HQT also resulted in microscopic amelioration of intestinal inflammation, as demonstrated by the reduction of MPO activity, a marker of mucosal neutrophil activation. These results are in agreement with those of previous studies, including our study that demonstrated alleviation of inflammation in TNBS-induced murine colitis by HQT1112. It has been reported that agents such as mesalazine, olsalazine and sulfasalazine are effective in preventing colitis in this animal model25.…”

Section: Discussionsupporting

confidence: 94%

“…It has been reported that agents such as mesalazine, olsalazine and sulfasalazine are effective in preventing colitis in this animal model25. The results of the current and previous studies suggest that HQT has a protective effect in DSS-induced colitis equal to or even greater than that of mesalazine (used as a reference in the present work) or sulfasalazine1112. Hence, HQT may be a promising candidate for the treatment of colitis.…”

Section: Discussionsupporting

confidence: 57%

“…It is known that chemokines such as MIP-3α, MCP-1 and MIP-2 are expressed specifically in the inflamed areas of the colon in patients with IBD1415. It is also known that HQT treatment decreases pro-inflammatory cytokine levels, including TNF-α, IL-1β and IL-6, in TNBS-induced colitis in rats11. Our current results obtained in mice with DSS-induced acute and chronic colitis further confirm this inhibitory effect of HQT on cytokines and chemokines.…”

Section: Discussionmentioning

confidence: 99%

“…HQT has been demonstrated to be clinically effective in the treatment of IBD. Our group and other researchers have demonstrated that HQT plays an important role in inhibiting intestinal inflammation and restoring the balance of CD4+T cells in experimental animal models with 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis1112. However, it is unclear whether HQT contributes to epithelial cell homeostasis and suppression of inflammation and immune response in other colitis models.…”

mentioning

confidence: 96%

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Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response

Zou

Lin

et al. 2016

Sci Rep

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Huangqin-tang (HQT) is a traditional Chinese medicine (TCM) formula widely used for the treatment of inflammatory bowel disease in China. However, the molecular mechanisms by which HQT protects the colon are unclear. We studied the protective effects of HQT and the underlying mechanisms in an experimental mouse model and in vitro. In vivo, dextran sodium sulphate (DSS)-induced acute and chronic colitis were significantly ameliorated by HQT as gauged by phenotypic, histopathologic and inflammatory manifestations of the disease. Mechanistically, DSS-induced nuclear factor-κB (NF-κB) signalling was inhibited by HQT. Moreover, HQT-treated mice demonstrated significant changes in cell apoptosis, expression of apoptosis-associated genes such as caspase-3, bax, bcl-2, and intestinal permeability. HQT also increased occluding and zonula occludens-1 (ZO-1), inhibited cell proliferation (Ki67), and increased regulatory T cells numbers, protein expression of Foxp3 and IL-10 in the colonic tissue. In vitro, HQT down-regulated production of pro-inflammatory cytokines and supressed the NF-κB signalling pathway in lipopolysaccharides-induced RAW 264.7 macrophages. Our study suggests that HQT plays a critical role in regulating intestinal epithelial cell homeostasis, inflammation and immune response in colitis and offers novel therapeutic options in the management of inflammatory bowel disease.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

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Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response

Zou

Lin

et al. 2016

Sci Rep

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“…Other reports20, 21, 22 also indicated that some components in SXD may contribute significantly to the anti-inflammatory bowel disease efficacy of this prescription. However, a detailed study on the profile of constituents of SXD formula has not yet been conducted.…”

Section: Introductionmentioning

confidence: 86%

Simultaneous determination of 14 active constituents of Shengjiang Xiexin decoction using ultrafast liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Peng

Guan

Wang

et al. 2017

Acta Pharmaceutica Sinica B

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An effective herbal medicinal prescription of Shengjiang Xiexin decoction (SXD) was used in treating the inflammatory bowel disease in clinic. In this study, an ultrafast liquid chromatography—tandem mass spectrometry (UFLC—MS/MS) method was developed to separate and to simultaneously determine 14 major active ingredients in SXD. Chromatographic separation was successfully accomplished on an Acquity BEH C18 (100 mm×2.1 mm, 1.7 μm) column using gradient elution with 0.1% (v/v) formic acid water (A) and 0.1% (v/v) formic acid in methanol (B). Negative and positive electrospray ionization tandem mass spectrometry was used to detect the 14 analytes using its selective reaction monitoring (SRM) mode. A good linear regression relationship for each analyte was obtained over the range from 3.88 ng/mL to 4080 ng/mL. The precision was evaluated by intra- and inter-day assays with a relative standard deviation (RSD) of less than 6.25%. The recovery measured at three concentration levels varied from 98.72% to 103.47%. The overall limits of quantification (LOQ) ranged from 2.05 ng/mL to 4.72 ng/mL. The method was successfully implemented in the qualitative and quantitative analyses of the 14 chemical constituents in SXD. The results showed that the developed UFLC—MS/MS method was linear and accurate. The method could be used reliably as a quality control method for SXD.

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Therapeutic Potential of Natural Agents Against Oxidative Stress-Influenced Colitis-Associated Cancer

Venkatesan

Sadik

Sivaprakasam

et al. 2022

Handbook of Oxidative Stress in Cancer: Therapeutic Aspects

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Anti-inflammatory effects of Huangqin tang extract in mice on ulcerative colitis

Cited by 53 publications

References 38 publications

Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response

Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response

Simultaneous determination of 14 active constituents of Shengjiang Xiexin decoction using ultrafast liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Therapeutic Potential of Natural Agents Against Oxidative Stress-Influenced Colitis-Associated Cancer

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