Anti-Inflammatory Effects of Deuterium-Depleted Water plus Rosa damascene Mill. Essential Oil via Cyclooxygenase-2 Pathway in Rats

Abstract

Objectives: Natural medicine has been proposed in treating of sepsis in the worldwide. So, in this study, the effect of deuterium-depleted water (DDW) alone and adjuvant with Rosa damascene Mill. essential oils (RD) was considered through the evaluation of oxidative stress/antioxidant parameters and the expression of cyclooxygenase-2 (COX-2) inflammatory gene in liver damage caused by sepsis. Materials and Methods: The rats were randomly divided into 5 groups: 1) laparotomy (LAP) group; 2) cecal ligation and puncture (CLP) group; 3) DDWs (15ppm and 30 ppm doses) groups; 4) DDWs (15ppm and 30 ppm doses) plus RD (100mg/kg.bw); 5) indomethacin (2 mg/kg.bw) as a positive control. The treatments were daily done for two weeks and creation of CLP model was occurred on the 15 th day. Then, the animals were killed and their liver tissue were separated for histopathologic and biochemical assessments. Results: Our results demonstrated that the treatment of animals with DDWs and DDWs plus RD were effective due to regulating the oxidative stress/antioxidant parameters including lipid peroxidation (LP), glutathione (GSH), glutathione s-transferases (GST), myeloperoxidase (MPO), ferric reducing ability of plasma (FRAP) and inflammatory parameters such as prostaglandin E2 (PGE2) and COX-2. Pathological studies also showed that sepsis led to the liver tissue injuries, which can be reduced by treatments. u n c o r r e c t e d p r o o f 2 Conclusion: Sepsis caused oxidative stress resulting in the liver tissue, but the administration of DDWs and DDWs plus RD can be useful to prevent and improve these injuries.

Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2

“…*These data are presented here in order to make a new compression among treatment groups (Fatemi et al. 2019).…”

“…c p < 0.05 is considered significantly between *DDWs treatment groups with DDW+ML groups *These data are presented here in order to make a new comparison among treatment groups (Fatemi et al. 2019).…”

“…2018; Fatemi et al. 2019). There is also an evidence to show the hepatoprotective activity of DDW against acetaminophen toxicity in in vivo system (Rasooli et al.…”

Effects of the Intake of Deuterium Depleted Water During Cancer Therapy: A Narrative Review

“…The isotopes of certain biogenic elements of a non-radioactive nature (Figure 2) such as hydrogen ( 1 H and 2 H), oxygen ( 16 O, 17 O, 18 O), carbon ( 12 С, 13 С) and nitrogen ( 14 N, 15 N); they cause a series of effects on the biochemical reactions and physiological processes of the organism, with a prominent influence on growth and development in both unicellular and multicellular organisms, as well as influencing energy metabolism and metabolic rate [3]. These reactions can affect the cell cycle and cell proliferation [4].…”

Relationships of irrigation water and soil physical and chemical characteristics with yield, chemical composition and antimicrobial activity of Damask rose essential oil

“…Results of clinical trials have shown that R. damascena EO has tranquilizing effects without any serious complications [14][15][16]. Various reports also demonstrated its biological activities including antioxidant [17,18], anti-cancer [19], anti-inflammatory [20], anti-HIV [21,22], antifungal, antibacterial, and antimicrobial [16,[23][24][25] properties.…”