Anti-Inflammatory Effects of Cycloheterophyllin on Dinitrochlorobenzene-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice

Abstract

Atopic dermatitis (eczema) is a condition that makes skin red and itchy. Though common in children, the condition can occur at any age. Atopic dermatitis is persistent (chronic) and tends to recur periodically. It may be accompanied by asthma or hay fever. No cure has been found for eczema. Therefore, it is very important to develop ingredients that aid the prevention and treatment of atopic dermatitis. Cycloheterophyllin is derived from Artocarpus heterophyllus and has antioxidant and anti-inflammatory activities. However, it still is not understood whether cycloheterophyllin is an anti-atopic dermatitis agent. Keratinocytes (HaCaT cells) and BALB/c mice for inducing AD-like cutaneous lesions were used to evaluate the potential of cycloheterophyllin as an anti-atopic dermatitis agent. The release of pro-inflammatory cytokines induced by treatment of TNF-α/IFN-γ was reduced after pretreatment with cycloheterophyllin. The inhibitory effects could be a contribution from the effect of the MAP kinases pathway. Moreover, the symptoms of atopic dermatitis (such as red skin and itching) were attenuated by pretreatment with cycloheterophyllin. Epidermal hyperplasia and mast cell infiltration were decreased in the histological section. Finally, damage to the skin barrier was also found to recover through assessment of transepidermal water loss. Taken together, prenylflavone-cycloheterophyllin from Artocarpus heterophyllus is a potential anti-atopic dermatitis ingredient that can be used in preventing or treating the condition.

Anti-Atopic Effect of Isatidis Folium Water Extract in TNF-α/IFN-γ-Induced HaCaT Cells and DNCB-Induced Atopic Dermatitis Mouse Model

“…Furthermore, WIF reduced serum IgE and TNF-α levels, which were elevated in the DNCB-induced group, alleviated epidermal and dermal thickness, and suppressed cell migration of inflammatory cells, such as eosinophils and mast cells. Based on these results, it can be considered that WIF exhibited anti-AD efficacy in a DNCB-induced AD-like mouse model [27]. Furthermore, WIF strongly inhibited ERK and p38 MAPKs activities and NF-κB-p65 translocation from the cytoplasm to the nucleus in the dorsal skin tissues of a DNCB-induced AD-like mouse model.…”

Anti-Atopic Dermatitis Activity of Epi-Oxyzoanthamine Isolated from Zoanthid

“…Many studies have shown that IFN-γ and TNF-α stimulate keratinocytes to induce signaling pathways involved in pro-inflammatory responses. Therefore, this model is often used as an in vitro test method for skin anti-inflammatory effectiveness [ 24 , 25 ]. It is shown in Figure 2 that once these pro-inflammatory cytokines (TNF-α and IFN-γ) are added, the formation of intracellular cytokine mRNA is significantly increased.…”

Arzanol, a natural phloroglucinol α‐pyrone, protects HaCaT keratinocytes against H2O2‐induced oxidative stress, counteracting cytotoxicity, reactive oxygen species generation, apoptosis, and mitochondrial depolarization

“…For the first time, in this study, the antioxidant effect of arzanol was natural extracts and compounds against oxidative stress in human epidermal cells and skin pathologies (Jiang et al, 2020;Mirata et al, 2023;Wang et al, 2022). In our experimental conditions, HaCaT cells were cultured in DMEM medium containing high calcium (Ca 2+ ) amount, a condition favorable for keratinocyte differentiation (Colombo et al, 2017).…”

“…For the first time, in this study, the antioxidant effect of arzanol was evaluated in human HaCaT keratinocytes against the oxidative stress induced by H 2 O 2 with the aim of exploring its beneficial potential effects on human skin oxidative conditions. Cultured human HaCaT cells, being physiologically similar to normal human keratinocytes, represent a cell model amply used to assess the protective effect of natural extracts and compounds against oxidative stress in human epidermal cells and skin pathologies (Jiang et al, 2020; Mirata et al, 2023; Wang et al, 2022). In our experimental conditions, HaCaT cells were cultured in DMEM medium containing high calcium (Ca 2+ ) amount, a condition favorable for keratinocyte differentiation (Colombo et al, 2017).…”