Anti-inflammatory effects of Chinese propolis in lipopolysaccharide-stimulated human umbilical vein endothelial cells by suppressing autophagy and MAPK/NF-κB signaling pathway

Han, Xuan; Yuan, Wenwen; Chang, Huasong; Liu, Minmin; Hu, Fuliang

doi:10.1007/s10787-018-0533-6

Cited by 21 publications

(11 citation statements)

References 37 publications

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“…Both Chinese propolis and Brazilian propolis have good immunomodulatory effects on LPS-induced inflammation. Chinese propolis modulates the suppression of autophagy and the MAPK/NF-κB signalling pathway to protect vascular endothelial cells from LPS stimulation [ 49 ]. Brazilian green propolis exerts strong anti-inflammatory effects by inhibiting the production of many cytokines, such as IL-1α, IL-1 β , IL-4, IL-6, TNF- α and MCP-1, in stimulated J774A cells [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

Yang

Wang

et al. 2020

Molecules

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Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties. To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays. The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting. The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4′-methoxy pinobanksin, were identified in EEP-B. Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time. The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 20 μg/mL EEP-B. This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression. These findings could provide a theoretical basis for MAEC treatment using EEP-B.

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Section: Discussionmentioning

confidence: 99%

The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

Yang

Wang

et al. 2020

Molecules

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“…This suggests that EEP treatment, which is a rich mixture of polyphenols, particularly pinocembrin, could be able to return to it basal levels of autophagy. Recently, it has been reported that Chinese propolis reduce the inflammation through an inhibition of autophagy by reducing the distribution and accumulation of LC3 in vascular endothelial cells [83]. Another study mentions that a Taiwanese green propolis partially inhibited the NLRP3 inflammasome via autophagy induction [84].…”

Section: Discussionmentioning

confidence: 99%

Propolis Reduces the Expression of Autophagy-Related Proteins in Chondrocytes under Interleukin-1β Stimulus

Arias

Saavedra

et al. 2019

IJMS

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Background: Osteoarthritis (OA) is a progressive and multifactorial disease that is associated with aging. A number of changes occur in aged cartilage, such as increased oxidative stress, decreased markers of healthy cartilage, and alterations in the autophagy pathway. Propolis extracts contain a mixture of polyphenols and it has been proved that they have high antioxidant capacity and could regulate the autophagic pathway. Our objective was to evaluate the effect of ethanolic extract of propolis (EEP) on chondrocytes that were stimulated with IL-1β. Methods: Rabbit chondrocytes were isolated and stimulated with IL-1β and treated with EEP. We evaluated cell viability, nitric oxide production, healthy cartilage, and OA markers, and the expression of three proteins associated with the autophagy pathway LC3, ATG5, and AKT1. Results: The EEP treatment reduces the expression of LC3, ATG5, and AKT1, reduces the production of nitric oxide, increases the expression of healthy markers, and reduces OA markers. Conclusions: These results suggest that treatment with EEP in chondrocytes that were stimulated with IL-1β has beneficial effects, such as a decrease in the expression of proteins associated with autophagy, MMP13, and production of nitric oxide, and also increased collagen II.

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“…Studies have found that propolis inhibits ox-LDL-induced ROS production and NF-κB p65 activation in human umbilical vein endothelial cells (Xuan et al, 2014). Meanwhile, propolis inhibits lipopolysaccharide (LPS)-induced TLR4 expression in vascular endothelial cells (Xuan et al, 2019). The results showed that propolis significantly reduced the activities of TLR4 and NF-κB p65, and inhibited the levels of NF-κB downstream factors TNFα and MMP-9, suggesting that propolis could improve the vascular inflammatory cascade through TLR4/NF-κB pathway.…”

Section: Discussionmentioning

confidence: 99%

Propolis ameliorates restenosis in hypercholesterolemia rabbits with carotid balloon injury by inhibiting lipid accumulation, oxidative stress, and TLR4/NF‐κB pathway

Ji¹,

Pan

et al. 2021

J. Food Biochem.

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Neointima formation and atherosclerosis are the main complications after the endovascular intervention and vascular surgery, and there are no effective drugs.Propolis is a kind of resin substance produced by honeybees and has numerous health-beneficial effects. In this study, we evaluated the effects of propolis (125 and 250 mg•kg -1 •day -1 , 6 weeks) on carotid restenosis in hypercholesterolemia rabbits.Propolis significantly ameliorated the degree of carotid restenosis, inhibited neointima hyperplasia, reduced serum lipids profile, and enhanced the anti-oxidative activities in hypercholesterolemia rabbits. Furthermore, propolis reduced the plasma levels of C-reactive protein, interleukin-6, and tumor necrosis factorα (TNFα), and inhibited the expression of CD68, TLR4, NF-κB p65, MMP-9, and TNFα in the carotid arteries. The results indicate that propolis has a protective effect on carotid restenosis in rabbits, which is associated with regulating blood lipids, inhibiting oxidative stress and inflammation, and its anti-inflammatory activity may be related to inhibit TLR4-mediated NF-κB signaling pathway. Practical applicationsRestenosis is a primary challenge in angioplasty and atherosclerotic treatment.Hyperlipidemia can induce inflammation and accelerate the formation of restenosis.Recently, natural products have been widely used to prevent intimal hyperplasia of common cardiovascular diseases. Propolis is currently a popular functional food, but the role of propolis on carotid restenosis after angioplasty and its underlying mechanism remains unclear. This study showed that propolis inhibits the effect of carotid restenosis in hypercholesterolemia rabbits. The results of this study may provide a basis for propolis to prevent and treat vascular restenosis.

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Anti-inflammatory effects of Chinese propolis in lipopolysaccharide-stimulated human umbilical vein endothelial cells by suppressing autophagy and MAPK/NF-κB signaling pathway

Cited by 21 publications

References 37 publications

The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

Propolis Reduces the Expression of Autophagy-Related Proteins in Chondrocytes under Interleukin-1β Stimulus

Propolis ameliorates restenosis in hypercholesterolemia rabbits with carotid balloon injury by inhibiting lipid accumulation, oxidative stress, and TLR4/NF‐κB pathway

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