Anti-inflammatory effects of Brucea javanica oil emulsion by suppressing NF-κB activation on dextran sulfate sodium-induced ulcerative colitis in mice

Huang, Yanfeng; Zhou, Jiangtao; Qu, Chang; Dou, Yao-Xing; Huang, Qionghui; Lin, Zhi‐Xiu; Xian, Yan‐Fang; Xie, Jianhui; Xie, Yinfeng; Lai, Xiaoping; Su, Zi‐Ren

doi:10.1016/j.jep.2017.01.042

Cited by 60 publications

(43 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IFNγ) pathway can increase expression of Smad7, 42,43 and local production of TNFα, IL-1β, and IFNγ was shown to be increased in UC patients and murine model of UC. 44,45 Our data demonstrated that treatment of experimental UC In conclusion, our study suggests that RF, which recovers the antioxidant status of the colonic tissue and ameliorates oxidative colonic injury by way of a neutrophil-dependent mechanism, merits further exploration as a promising therapeutic and/or prophylactic agent in UC. Since RF is relatively inexpensive and essentially non-toxic, clinical trials should evaluate its antioxidant and anti-inflammatory potential for the treatment of inflammatory diseases involving acute oxidative damage.…”

Section: Discussionmentioning

confidence: 57%

“…TNF‐α and IL‐1β) or STAT‐1 (e.g. IFN‐γ) pathway can increase expression of Smad7, and local production of TNF‐α, IL‐1β, and IFN‐γ was shown to be increased in UC patients and murine model of UC . Our data demonstrated that treatment of experimental UC rats with RF increased the TGF‐β1 protein expressions likely through the amplification of the TGF‐β1/Smad3 signalling cascade and/or inhibition of the proinflammatory mediators (e.g.…”

Section: Discussionmentioning

confidence: 58%

See 1 more Smart Citation

Ameliorative effects of riboflavin on acetic acid‐induced colonic injury in rats

Karakoyun

Ertaş

Yüksel

et al. 2017

Clin Exp Pharma Physio

View full text Add to dashboard Cite

Riboflavin (RF) has been found to be a promising antioxidant and/or anti-inflammatory agent in several studies. However, the effect of RF against acetic acid (AA)-induced colonic injury is currently unknown. This study aimed to investigate the potential antioxidant and protective effects of RF in a rat model of ulcerative colitis. Starting immediately after the colitis induction (AA+RF group) or 1 week before the colitis induction (RF+AA+RF group), the rats were treated with RF (25 mg/kg per day; p.o.) for 3 days. The control and AA groups received saline (1 mL; p.o.) whereas AA+SS group (positive control) received sulfasalazine (100 mg/kg per day; p.o.) for 3 days. Colonic samples were taken for the biochemical and histological assessments on the third day. High damage scores, elevated tissue wet weight index (WI), tissue myeloperoxidase (MPO) activity, 8-hydroxy-2'-deoxyguanosine levels and chemiluminescence values, and a pronounced decrease in antioxidant glutathione (GSH) levels of the AA group were all reversed by RF pretreatment (RF+AA+RF group) and SS treatment (AA+SS group) (P < .05-.001). Tissue WI, MPO activity and GSH levels were not statistically changed in the AA+RF group. Western blot analysis revealed that the decreased protein expressions of tissue collagen (COL) 1A1, COL3A1 and transforming growth factor-β1 in the AA group were elevated in all the treatment groups (P < .05-.001). In conclusion, RF exerts both the antioxidant and anti-inflammatory effects against AA-induced colonic inflammation by suppressing neutrophil accumulation, inhibiting reactive oxidant generation, preserving endogenous glutathione, improving oxidative DNA damage and regulating inflammatory mediators, suggesting a future potential role in the treatment and prevention of ulcerative colitis.

show abstract

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 58%

Ameliorative effects of riboflavin on acetic acid‐induced colonic injury in rats

Karakoyun

Ertaş

Yüksel

et al. 2017

Clin Exp Pharma Physio

View full text Add to dashboard Cite

show abstract

“…It suggests that BR treatment is able to alleviate a certain degree of symptoms in mice with colonic inflammation by regulation of anti-inflammatory status [81]. Additionally, the ethyl acetate extract of the ripe fruit of B. javanica, brucea javanica oil (BJO) (0.5 1 and 2 g/kg) treatment reduces the body weight loss, restores the colon length and decreases the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17 and IFN-γ via the suppression of NF-κB pathway in DSS-induced mice [82].…”

Section: Detoxificating and Purgative Medicinementioning

confidence: 99%

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

Zhao

2020

Chin Med

View full text Add to dashboard Cite

Nuclear factor-kappa B (NF-κB) is a kind of multi-functional nuclear transcription factor involved in regulating gene transcription to influence pathological evolution of inflammatory and immune diseases. Numerous literature evidence that NF-κB pathway plays an essential role in pathogenic development of ulcerative colitis (UC). UC is a chronic non-specific inflammatory bowel disease, and until now, therapeutic agents for UC including aminosalicylates, corticosteroids and immune inhibitors still cannot exert satisfied effects on patients. In recent years, Chinese medicines suggest the advantages of alleviating symptoms and signs, decreasing side-effects and recurrence, whose one of mechanisms is related to regulation of NF-κB pathway. In this review, we categorize Chinese medicines according to their traditional therapeutic functions, and summarize the characteristics of Chinese medicines targeting NF-κB pathway in UC treatment. It indicates that 85 kinds of Chinese medicines' compounds and formulae can directly act on NF-κBp65; while 58 Chinese medicines' ingredients and formulae indirectly suppress NF-κBp65 by regulation of its upstream or other related pathways. Moreover, by the analysis of Chinese medicines' category based on their traditional functions, we conclude the category of dampness-drying and detoxificating medicine targeting NF-κB pathway accounts for primary status for amelioration of UC. Simultaneously, this review also contributes to the choices of Chinese medicine category and provides curative potential of Chinese medicines for clinical UC treatment.

show abstract

“…7 The desiccative ripe fruit of B. javanica (Ya-dan-zi in Chinese) is a famous traditional Chinese folk medicine commonly used to treat dysentery, malaria, hemorrhoids, corns, and other diseases. 8 Bruceine D (BD, C 20 H 26 O 9 ; the chemical structure is shown in Figure 1A) is a natural quassinoid isolated from B. javanica fruit. 9 BD has been reported to have various pharmacological activities including antipancreatic cancer, 10 anti-hepatocellular carcinoma, 11 human papillomavirus inhibitory effect, and so on.…”

Section: Introductionmentioning

confidence: 99%

Self-nanoemulsfiying drug delivery system of bruceine D: a new approach for anti-ulcerative colitis

Dou¹,

Zhou²,

Wang³

et al. 2018

IJN

Self Cite

View full text Add to dashboard Cite

BackgroundBruceine D (BD) is a major bioactive component isolated from the traditional Chinese medicinal plant Brucea javanica which has been widely utilized to treat dysentery (also known as ulcerative colitis [UC]).MethodsTo improve the water solubility and absolute bioavailability of BD, we developed a self-nanoemulsifying drug delivery system (SNEDDS) composing of MCT (oil), Solutol HS-15 (surfactant), propylene glycol (co-surfactant) and BD. The physicochemical properties and pharmacokinetics of BD-SNEDDS were characterized, and its anti-UC activity and potential mechanism were evaluated in TNBS-induced UC rat model.ResultsThe prepared nanoemulsion has multiple beneficial aspects including small mean droplet size, low polydispersity index (PDI), high zeta potential (ZP) and excellent stability. Transmission electron microscopy showed that nanoemulsion droplets contained uniform shape and size of globules. Pharmacokinetic studies demonstrated that BD-SNEDDS exhibited enhanced pharmacokinetic parameters as compared with BD-suspension. Moreover, BD-SNEDDS significantly restored the colon length and body weight, reduced disease activity index (DAI) and colon pathology, decreased histological scores, diminished oxidative stress, and suppressed TLR4, MyD88, TRAF6, NF-κB p65 protein expressions in TNBS-induced UC rat model.ConclusionThese results demonstrated that BD-SNEDDS exhibited highly improved oral bioavailability and advanced anti-UC efficacy. In conclusion, our current results provided a foundation for further research of BD-SNEDDS as a potential complementary therapeutic agent for UC treatment.

show abstract

Anti-inflammatory effects of Brucea javanica oil emulsion by suppressing NF-κB activation on dextran sulfate sodium-induced ulcerative colitis in mice

Cited by 60 publications

References 38 publications

Ameliorative effects of riboflavin on acetic acid‐induced colonic injury in rats

Ameliorative effects of riboflavin on acetic acid‐induced colonic injury in rats

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

Self-nanoemulsfiying drug delivery system of bruceine D: a new approach for anti-ulcerative colitis

Contact Info

Product

Resources

About