Anti-Inflammatory Effects of Alpha Linolenic Acid on Human Corneal Epithelial Cells

Erdinest, Nir; Shmueli, Or; Grossman, Yoni; Ovadia, Haim; Solomon, Abraham

doi:10.1167/iovs.12-9724

Cited by 93 publications

(69 citation statements)

References 39 publications

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“…91 IL-8 is also an important inflammatory mediator during infection, 92 and is increased in corneal epithelial cells in response to lipopolysaccharide stimulation. 93 Santacruz et al reported that IL-1β, IL-6 and IL-8 were elevated in tears from eyes with infectious keratitis, compared with those in the control contralateral eyes. 94 …”

Section: Bilateral Alteration In Tear Cytokinesmentioning

confidence: 98%

Bilateral Alterations in Corneal Nerves, Dendritic Cells, and Tear Cytokine Levels in Ocular Surface Disease

Yamaguchi

Hamrah

Shimazaki

2016

Cornea

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This review summarizes recent literature regarding corneal imaging in human subjects using in vivo confocal microscopy and corneal immune cells, nerves, and tear cytokine levels in ocular surface diseases as well as corneal immune privilege. The significance of interactions between corneal immune cells and nerves in health, neurotrophic keratopathy, and infectious keratitis are discussed. Furthermore, bilateral alterations of immune cells and nerves in clinically unilateral corneal diseases and the link to changes of tear cytokines or neuropeptide levels in contralateral eyes are described. Recent studies reported increased density and morphologic changes of corneal dendritic cells in ocular surface disease that correlated with a decrease in sub-basal nerve corneal nerves, suggesting potential interactions between the immune and nervous systems in the cornea. Although the relevance of tear cytokines is poorly understood, tear cytokines might have an important role in the pathogenesis of ocular surface diseases. In humans and experimental animal models, alterations in immune cells, cytokines and immunomodulatory neuropeptide levels in contralateral eyes might mediate the incidence of bilateral infectious keratitis and loss of immune privilege of the cornea in bilateral corneal transplantation or neurotrophic keratopathy cases. The discovery of bilateral alterations of immune cells and nerves in ocular surface diseases is considered the missing link between the immune and nervous systems in the cornea, and demonstrates how studies of animal models and human patients aid our understanding of human corneal disease phenomena.

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Section: Bilateral Alteration In Tear Cytokinesmentioning

confidence: 98%

Bilateral Alterations in Corneal Nerves, Dendritic Cells, and Tear Cytokine Levels in Ocular Surface Disease

Yamaguchi

Hamrah

Shimazaki

2016

Cornea

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“…Nutritionally, C20:5 eicosapentaenoic acid (EPA) and C22:6 docohexaenoic acid (DHA) are the most important VLC-PUFAs belonging to the v-3 family. Out of the v-6 family, C18:1 alfa linoleic acid (ALA) [2] and C20:4 arachidonic acid (AA) [3] have also been shown to have health benefits. There is a considerable amount of research that supports the beneficial health properties of VLC-PUFAs, particularly the v-3 FAs [4].…”

Section: Introductionmentioning

confidence: 99%

Towards sustainable sources for omega-3 fatty acids production

Adarme-Vega

Thomas‐Hall

Schenk

2014

Current Opinion in Biotechnology

197

111

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“…Rashid et al’s study has shown that the topical administration of omega-3 fatty acid in DES mouse model decreased the number of CD11b + cells and the levels of pro-inflammatory cytokines such as IL-1α and TNF-α in the ocular surface. 7 Recently, a study we have conducted had shown that administration of Alpha linolenic acid (ALA) to HCE cells stimulated by Lipopolysaccharide (LPS) or polyriboinosinic:polyribocytidylic acids (poly I:C) in-vitro significantly reduced the protein and mRNA levels of several pro-inflammatory cytokines [13]. …”

Section: Discussionmentioning

confidence: 99%

“…Both protein and mRNA levels of several pro-inflammatory cytokines were dramatically decreased following treatment with ALA [13]. The results of that study have paved the way for further search of PUFAs bioactive derivatives, which may have a stronger anti-inflammatory effect.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effects of resolvin-D1 on human corneal epithelial cells: in vitro study

et al. 2014

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BackgroundThis study evaluated the anti-inflammatory effects of Resolvin-D1 (RV-D1) and its mechanism of action in human corneal epithelial (HCE) cells.MethodsHCE cells were incubated with different concentrations of RV-D1 for different time periods. Oleic acid (OA) and Dexamethasone (DM) served as negative and positive controls, respectively. Cells were stimulated with polyriboinosinic:polyribocytidylic acids (poly I:C). The protein contents and mRNA expression levels of Tumor necrosis factor-α (TNF-α), Interleukin (IL)-6, IL-1β and IL-8 were evaluated with multiplex fluorescent bead immunoassay (FBI) and real time-PCR, respectively. In addition, the expression of inhibitory factor-κBα (I-κBα) was evaluated with real time-PCR.ResultsThe protein level of pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IL-8 significantly increased after stimulation with Poly I:C. RV-D1 treatment at concentration of 1 μM decreased the protein level of TNF-α to 20.76 ± 9.3% (P < 0.05), IL-6 to 43.54 ± 14.16% (P < 0.001), IL-1β to 46.73 ± 15.93% (P > 0.05) and IL-8 to 51.15 ± 13.01% (P < 0.05) compared with cells stimulated with poly I:C alone. Similarly, the mRNA levels of TNF-α, IL-6, IL-1β and IL-8 were significantly reduced after treatment with RV-D1. A highly significant dose response curve was demonstrated for RV-D1 treated HCE cells for TNF-α and IL-1β.DM treatment decreased the protein content for all of the pro-inflammatory cytokines, similar results were demonstrated at the mRNA level. The anti-inflammatory effects of RV-D1 were similar to those of DM for TNF-α, IL-6 and IL-8.ConclusionsRV-D1 may serve as a potent anti-inflammatory agent in ocular surface inflammation, as evaluated in cultured HCE cells. The anti-inflammatory effects of RV-D1 were comparable to those of DM, and were mediated through nuclear factor kappa B (NF-κB) signal transduction.

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Anti-Inflammatory Effects of Alpha Linolenic Acid on Human Corneal Epithelial Cells

Abstract: ALA may serve as a potent anti-inflammatory agent in ocular surface inflammation. The anti-inflammatory effects of ALA are comparable to those of corticosteroids, and are mediated through NF-κB signal transduction.

Cited by 93 publications

References 39 publications

Bilateral Alterations in Corneal Nerves, Dendritic Cells, and Tear Cytokine Levels in Ocular Surface Disease

Bilateral Alterations in Corneal Nerves, Dendritic Cells, and Tear Cytokine Levels in Ocular Surface Disease

Towards sustainable sources for omega-3 fatty acids production

Anti-inflammatory effects of resolvin-D1 on human corneal epithelial cells: in vitro study

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