Anti-inflammatory effects of aloe vera on soy meal-induced intestinal inflammation in zebrafish

Fehrmann-Cartes, Karen; Coronado, Maximo; Hernández, Adrián; Allende, Miguel L.; Feijóo, Carmen G.

doi:10.1016/j.fsi.2019.10.075

Cited by 29 publications

(23 citation statements)

References 47 publications

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“…In addition, dietary challenges may be able to trigger intestine immune response by the activation of a set of pro-and anti-inflammatory-related genes, which may provide information on gut health even in the absence of clear histo-pathological evidence [12,13]. Cytokines such as interleukins and tumor necrosis factor a (tnf-a) and inflammation mediators such as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and myeloid differentiation primary response 88 (MyD88) represent useful markers of inflammation that are able to provide early information when testing new ingredients in aquafeed formulations [14,15].…”

Section: Introductionmentioning

confidence: 99%

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Randazzo

Zarantoniello

Cardinaletti

et al. 2021

Animals

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The attempt to replace marine-derived ingredients for aquafeed formulation with plant-derived ones has met some limitations due to their negative side effects on many fish species. In this context, finding new, sustainable ingredients able to promote fish welfare is currently under exploration. In the present study, poultry by-products and Hermetia illucens meal were used to replace the vegetable protein fraction in diets totally deprived of fish meal intended for gilthead seabream (Sparus aurata). After a 12-week feeding trial, a multidisciplinary approach including histological, molecular, and spectroscopic techniques was adopted to investigate intestine and liver responses to the different dietary formulations. Regardless of the alternative ingredient used, the reduction in dietary vegetable proteins resulted in a lower incidence of intestine histological alterations and inflammatory responses. In addition, the dietary inclusion of insect meal positively affected the reduction in the molecular inflammatory markers analyzed. Spectroscopic analyses showed that poultry by-product meal improved lipid absorption in the intestine, while insect meal induced increased liver lipid deposition in fish. The results obtained demonstrated that both poultry by-products and H. illucens meal can successfully be used to replace plant-derived ingredients in diets for gilthead seabream, promoting healthy aquaculture.

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Section: Introductionmentioning

confidence: 99%

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Randazzo

Zarantoniello

Cardinaletti

et al. 2021

Animals

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“…As fishmeal remains an important source of dietary protein in aquaculture diets, its increasing price and decreasing availability have given birth to a replacement of feed made from plant sources of dietary protein, such as soybean meal ( 1 ). The side effects resulting from plant-sourced proteins have occurred in many fish species, from carnivorous to herbivorous, such as salmon ( 2 ), grouper ( 3 ), zebrafish ( 4 , 5 ), and carp ( 6 ). Fish foodborne enteritis also is due to the dysregulation of oral tolerance, which is related to immunoregulation locally at the intestinal mucosa ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition

Xie

et al. 2021

Front. Immunol.

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Foodborne intestinal inflammation is a major health and welfare issue in aquaculture. To prevent enteritis, various additives have been incorporated into the fish diet. Considering anti-inflammatory immune regulation, an effective natural compound could potentially treat or prevent intestinal inflammation. Our previous study has revealed galantamine’s effect on soybean induced enteritis (SBMIE) and has highlighted the possible role of the cholinergic anti-inflammatory pathway in the fish gut. To further activate the intestinal cholinergic related anti-inflammatory function, α7nAchR signaling was considered. In this study, sinomenine, a typical agonist of α7nAChR in mammals, was tested to treat fish foodborne enteritis via its potential anti-inflammation effect using the zebrafish foodborne enteritis model. After sinomenine’s dietary inclusion, results suggested that there was an alleviation of intestinal inflammation at a pathological level. This outcome was demonstrated through the improved morphology of intestinal villi. At a molecular level, SN suppressed inflammatory cytokines’ expression (especially for tnf-α) and upregulated anti-inflammation-related functions (indicated by expression of il-10, il-22, and foxp3a). To systematically understand sinomenine’s intestinal effect on SBMIE, transcriptomic analysis was done on the SBMIE adult fish model. DEGs (sinomenine vs soybean meal groups) were enriched in GO terms related to the negative regulation of lymphocyte/leukocyte activation and alpha-beta T cell proliferation, as well as the regulation of lymphocyte migration. The KEGG pathways for glycolysis and insulin signaling indicated metabolic adjustments of α7nAchR mediated anti-inflammatory effect. To demonstrate the immune cells’ response, in the SBMIE larva model, inflammatory gatherings of neutrophils, macrophages, and lymphocytes caused by soybean meal could be relieved significantly with the inclusion of sinomenine. This was consistent within the sinomenine group as CD4+ or Foxp3+ lymphocytes were found with a higher proportion at the base of mucosal folds, which may suggest the Treg population. Echoing, the sinomenine group’s 16s sequencing result, there were fewer enteritis-related TM7, Sphingomonas and Shigella, but more Cetobacterium, which were related to glucose metabolism. Our findings indicate that sinomenine hydrochloride could be important in the prevention of fish foodborne enteritis at both immune and microbiota levels.

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“…The symptoms of SBMIE are most apparent in the posterior/distal intestine of turbot ( 6 , 7 ) and grass carp ( 5 ), the most important mucosal immune organ ( 8 ). Fish SBMIE has been found to be accompanied by a decrease in the height of villi and microvilli, downregulation of tight junction protein claudin-4, occluding , and ZO-1 mRNA levels, and upregulation of pro-inflammatory cytokine genes, including TNF- α, IL-1 β, IL-8 , and IL-16 ( 9 – 12 ), influencing the innate immune response. Zebrafish SBMIE is T cell-dependent and has a T helper (Th) 17 cytokine profile ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Integrative Transcriptomic and Small RNA Sequencing Reveals Immune-Related miRNA–mRNA Regulation Network for Soybean Meal-Induced Enteritis in Hybrid Grouper, Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂

Chi

et al. 2020

Front. Immunol.

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A 10-week feeding experiment was conducted to reveal the immune mechanism for soybean meal-induced enteritis (SBMIE) in hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂. Four isonitrogenous and isolipidic diets were formulated by replacing 0, 10, 30, and 50% fish meal protein with soybean meal (namely FM, SBM10, SBM30, and SBM50, respectively). The weight gain rate of the SBM50 group was significantly lower than those of the other groups. Plica height, muscular layer thickness, and goblet cells of the distal intestine in the SBM50 group were much lower than those in the FM group. The intestinal transcriptomic data, including the transcriptome and miRNAome, showed that a total of 6,390 differentially expressed genes (DEGs) and 92 DEmiRNAs were identified in the SBM50 and FM groups. DEmiRNAs (10 known and 1 novel miRNAs) and their DE target genes were involved in immune-related phagosome, natural killer cell-mediated cytotoxicity, Fc gamma R-mediated phagocytosis, and the intestinal immune network for IgA production pathways. Our study is the first to offer transcriptomic and small RNA profiling for SBMIE in hybrid grouper. Our findings offer important insights for the understanding of the RNA profile and further elucidation of the underlying molecular immune mechanism for SBMIE in carnivorous fish.

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Anti-inflammatory effects of aloe vera on soy meal-induced intestinal inflammation in zebrafish

Cited by 29 publications

References 47 publications

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition

Integrative Transcriptomic and Small RNA Sequencing Reveals Immune-Related miRNA–mRNA Regulation Network for Soybean Meal-Induced Enteritis in Hybrid Grouper, Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂

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