Anti-Inflammatory Effects of Adenine Enhance Osteogenesis in the Osteoblast-Like MG-63 Cells

Chen, Yu Pin; Chu, Yo Lun; Tsuang, Yang Hwei; Wu, Yueh Lin; Kuo, Chao‐Yin; Kuo, Yi Jie

doi:10.3390/life10070116

Cited by 10 publications

(13 citation statements)

References 48 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DNA hydrogel was able to promote new bone formation, even though it degraded relatively early on (as judged by the in vitro experiments). This supports the hypothesis that DNA exerts a rather early osteogenic inductive effect, which can potentially be heightened as DNA degrades and releases extracellular phosphate ions from its phosphate-rich backbone and likely adenine, which have been shown to induce migration and up-regulate osteogenic differentiation ( 21 – 28 ). The ratio of bone volume to tissue volume (BV/TV) in the region of interest was measured for each sample to characterize the amount of trabecular bone formed in the calvarial defect.…”

Section: Resultssupporting

confidence: 80%

“…This value corresponds to a concentration of 3.1 mM of adenine in the culture medium. Previously, it was reported that adenine up-regulates osteogenic genes using exogenous adenine supplementation of 100 µM for 24 h ( 28 ).…”

Section: Resultsmentioning

confidence: 99%

“…Notably, extracellular phosphate can promote osteogenic differentiation and calcification of preosteoblasts and mesenchymal stem cells ( 21 – 23 ). Extracellular adenine, another side product of DNA degradation, has also been shown to be a promoter of osteogenic differentiation ( 24 – 28 ).…”

mentioning

confidence: 99%

See 2 more Smart Citations

DNA hydrogels for bone regeneration

Athanasiadou

Meshry

Monteiro

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

DNA-based biomaterials have been proposed for tissue engineering approaches due to their predictable assembly into complex morphologies and ease of functionalization. For bone tissue regeneration, the ability to bind Ca 2+ and promote hydroxyapatite (HAP) growth along the DNA backbone combined with their degradation and release of extracellular phosphate, a known promoter of osteogenic differentiation, make DNA-based biomaterials unlike other currently used materials. However, their use as biodegradable scaffolds for bone repair remains scarce. Here, we describe the design and synthesis of DNA hydrogels, gels composed of DNA that swell in water, their interactions in vitro with the osteogenic cell lines MC3T3-E1 and mouse calvarial osteoblast, and their promotion of new bone formation in rat calvarial wounds. We found that DNA hydrogels can be readily synthesized at room temperature, and they promote HAP growth in vitro, as characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. Osteogenic cells remain viable when seeded on DNA hydrogels in vitro, as characterized by fluorescence microscopy. In vivo, DNA hydrogels promote the formation of new bone in rat calvarial critical size defects, as characterized by micro-computed tomography and histology. This study uses DNA hydrogels as a potential therapeutic biomaterial for regenerating lost bone.

show abstract

Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

DNA hydrogels for bone regeneration

Athanasiadou

Meshry

Monteiro

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Immature osteoblasts produce many specific proteins, including alkaline phosphatase, collagen type I, osteopontin and bone sialoprotein and so block their potential to differentiate into the chondrocytic and adipocytic lineage [ 31 , 35 ].…”

Section: Osteogenic Differentiation and Its Testingmentioning

confidence: 99%

“…ALP is a membrane-bound enzyme expressed in immature osteoblast cells. It is considered to be a marker for their early differentiation and plays a key role in the calcification of bones [ 35 ]. ALP hydrolyzes pyrophosphate and supplies inorganic phosphate to enhance mineralization as well as to reduce extracellular pyrophosphate, an inhibitor of mineral formation [ 36 ].…”

Section: Osteogenic Differentiation and Its Testingmentioning

confidence: 99%

Human cells with osteogenic potential in bone tissue research

et al. 2023

View full text Add to dashboard Cite

Bone regeneration after injury or after surgical bone removal due to disease is a serious medical challenge. A variety of materials are being tested to replace a missing bone or tooth. Regeneration requires cells capable of proliferation and differentiation in bone tissue. Although there are many possible human cell types available for use as a model for each phase of this process, no cell type is ideal for each phase. Osteosarcoma cells are preferred for initial adhesion assays due to their easy cultivation and fast proliferation, but they are not suitable for subsequent differentiation testing due to their cancer origin and genetic differences from normal bone tissue. Mesenchymal stem cells are more suitable for biocompatibility testing, because they mimic natural conditions in healthy bone, but they proliferate more slowly, soon undergo senescence, and some subpopulations may exhibit weak osteodifferentiation. Primary human osteoblasts provide relevant results in evaluating the effect of biomaterials on cellular activity; however, their resources are limited for the same reasons, like for mesenchymal stem cells. This review article provides an overview of cell models for biocompatibility testing of materials used in bone tissue research.

show abstract