Anti-inflammatory effect of tricin 4′-O-(threo-β-guaiacylglyceryl) ether, a novel flavonolignan compound isolated from Njavara on in RAW264.7 cells and in ear mice edema

Jung, Young Suk; Kim, Dae Hwan; Hwang, Jin-Hee; Yun, Na Young; Lee, Yun Hee; Han, Sang Bae; Hwang, Bang Yeon; Lee, Moon Soon; Jeong, Heon Sang; Hong, Jin Tae

doi:10.1016/j.taap.2014.03.001

Cited by 42 publications

(22 citation statements)

References 48 publications

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“…Treatment with 20 lg/mL tricin 4 0 -O-(threo-b-guaiacylglyceryl) ether (TTGE) was shown to decrease expression levels of iNOS and COX-2 by about 30% (Jung et al 2014). Forstermann et al (1995) also reported that the expression of iNOS was mainly regulated at the transcription level which was consistent with those reported by Forstermann et al (1995).…”

Section: Effect Of Tricin On the Production Of No And Pge 2 In Lps-stsupporting

confidence: 75%

AMP Kinase Activation and Inhibition of Nuclear Factor-Kappa B (NF-κB) Translocation Contribute to the Anti-Inflammatory Effect of Tricin

Lee

Imm

2016

Journal of Food Biochemistry

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Tricin is a methylated flavone widely distributed in cereal crops. The mechanism underlying the anti-inflammatory effects of tricin was examined using RAW 264.7 macrophages. Tricin up to 50 lg/mL showed no cytotoxic effects and significantly decreased the levels of nitric oxide (NO) and prostaglandin E2 (PGE 2 ). The inhibition of inducible NO synthase (iNOS) and cyclooxygenase (COX-2) expression were determined by western blotting. The level of iNOS expression was decreased by 80% with tricin concentration as low as 3 lg/mL. Moreover, treatment with tricin significantly decreased the production of pro-inflammatory cytokines, including tumor necrosis factor a (TNF-a), interlukin 1b (IL-1b), and IL-6. These effects were mediated by inhibition of the nuclear factor-kappa B (NFjB) translocation through stimulation of adenosine monophosphate-activated protein kinase (AMPK) in macrophages. PRACTICAL APPLICATIONSTricin is a flavonoid found in the outer layer of cereal crops including cereal byproducts. This study demonstrated that anti-inflammatory activity mediated by tricin was related to AMPK activation that inhibits NF-jB translocation. Since AMPK activation is a promising target to modulate lipid and glucose metabolism, tricin has great potential as anti-diabetic and anti-obesity agent. Cereal bran fraction containing high tricin content can be a practical source to deliver valuable bioactive compounds.

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Section: Effect Of Tricin On the Production Of No And Pge 2 In Lps-stsupporting

confidence: 75%

AMP Kinase Activation and Inhibition of Nuclear Factor-Kappa B (NF-κB) Translocation Contribute to the Anti-Inflammatory Effect of Tricin

Lee

Imm

2016

Journal of Food Biochemistry

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“…Macrophage stimulated with LPS could increase the release of pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6 (Chen et al, 2017). In addition, LPS can also stimulate the over production of ROS and nitric oxide (NO), which aggravates oxidative stress in RAW264.7 cells (Jung et al, 2014). Interestingly, our results showed that T11 treatment could dose-dependently inhibit the secretion of pro-inflammation cytokines (i.e., TNF-α, IL-1β and IL-6) in LPS-stimulated RAW264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

Triptriolide Alleviates Lipopolysaccharide-Induced Liver Injury by Nrf2 and NF-κB Signaling Pathways

et al. 2018

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Nrf2 (Nuclear Factor Erythroid 2 Related Factor 2) transcription factor not only regulates oxidative stress response, but also represses inflammation by regulating cytokines production and cross-talking with NF-κB signaling pathways. Nrf2 plays an essential role in liver injury induced by oxidative stress and inflammation. Triptriolide (T11) is a minor component of Tripterygium wilfordii Hook F. (TwHF), which can be obtained by hydrolysis reaction of triptolide (T9). The major purpose of this study is to clarify the regulating effects of T11 on oxidative stress and inflammation in vivo and in vitro. LPS-stimulated RAW 264.7 cells were used to verify the regulating effects of T11 on oxidative stress (ROS and Nrf2 signaling pathway) and inflammatory cytokines production (TNF-α, IL-6 and IL-1β). The antioxidant responsive element (ARE) luciferase assay was employed to evaluate Nrf2 activation effect of T11 in HEK-293T cells. Lipopolysaccharides (LPS) induced acute liver injury (ALI) in BALB/c mice were used to study the protective effects (ALT, AST, MDA, SOD, histopathology and neutrophils/macrophages filtration) and the underlying protection mechanisms of ALI amelioration (Nrf2 and NF-κB signaling pathway) of T11. Firstly, the results showed that T11 can not only effectively decrease the productions of inflammatory cytokines (TNF-α, IL-6 and IL-1β), ROS and NO in LPS-stimulated RAW 264.7 cells, but also further significantly increase the activity of Nrf2 in HEK-293T cells. Secondly, the results suggested that T11 could dramatically decrease the oxidative stress responses (SOD and MDA) and inflammation (histopathology, neutrophils/macrophages filtration, TNF-α, IL-6 and IL-1β production) in LPS-induced ALI in BALB/c mice. Finally, the results implied that T11 could dramatically increase Nrf2 protein expression and decrease p-TAK1, p-IκBα and NF-κB protein expression both in vivo and in vitro. In conclusion, our findings indicated that T11 could alleviate LPS induced oxidative stress and inflammation by regulating Nrf2 and NF-κB signaling pathways in vitro and in vivo, which offers a novel insights for the application of TwHF in clinical.

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“…Thus, alpa and beta configuration of structures were omitted like several references. 30,31) Among the isolated compounds, the new flavonolignan salcolin D showed the strongest anti-inflammatory and anti-allergic activities. Therefore, we believe that salcolin D is the active constituent responsible for the efficacy of Z. latifolia.…”

Section: Discussionmentioning

confidence: 99%

Tricin derivatives as anti-inflammatory and anti-allergic constituents from the aerial part of Zizania latifolia

Lee

Baek

Eun

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Methanol extract of Zizania latifolia was partitioned with EtOAc, n-BuOH, and H2O. From the EtOAc layers, a new flavonolignan along with a known flavone and three known flavonolignans, tricin (1), salcolin A (2), salcolin B (3), and salcolin C (4), were isolated through repeated silica gel and ODS column chromatography. The chemical structure of the new flavonolignan was determined to be tricin-4′-O-[erythro-β-guaiacyl-(7″-O-methyl)-glyceryl] ether and was named salcolin D (5) based on physicochemical and spectroscopic data, including FT-NMR and ESI-MS. All compounds were isolated for the first time from this plant. Compounds 2–5, tricin derivatives, all exhibited higher anti-inflammatory and anti-allergy activities than tricin. In particular, salcolin D (5) was shown to have the strongest inhibitory activity against LPS-induced NO production in RAW 264.7 cells as well as β-hexosaminidase release in IgE-sensitized RBL-2H3 cells. These results suggest that the presence of tricin derivatives conveys allergy and inflammation treatment ability to Z. latifolia.

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Anti-inflammatory effect of tricin 4′-O-(threo-β-guaiacylglyceryl) ether, a novel flavonolignan compound isolated from Njavara on in RAW264.7 cells and in ear mice edema

Cited by 42 publications

References 48 publications

AMP Kinase Activation and Inhibition of Nuclear Factor-Kappa B (NF-κB) Translocation Contribute to the Anti-Inflammatory Effect of Tricin

AMP Kinase Activation and Inhibition of Nuclear Factor-Kappa B (NF-κB) Translocation Contribute to the Anti-Inflammatory Effect of Tricin

Triptriolide Alleviates Lipopolysaccharide-Induced Liver Injury by Nrf2 and NF-κB Signaling Pathways

Tricin derivatives as anti-inflammatory and anti-allergic constituents from the aerial part of Zizania latifolia

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