Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model

Abstract

Centella asiatica has potent antioxidant and anti-inflammatory properties. However, its anti-dermatitic effect has not yet been reported. In this study, we investigated the anti-dermatitic effects of titrated extract of Centella asiatica (TECA) in a phthalic anhydride (PA)-induced atopic dermatitis (AD) animal model as well as in vitro model. An AD-like lesion was induced by the topical application of five percent PA to the dorsal skin or ear of Hos:HR-1 mouse. After AD induction, 100 μL of 0.2% and 0.4% of TECA (40 μg or 80 μg/cm2) was spread on the dorsum of the ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression by Western blotting for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-κB activity, which were determined by electromobility shift assay (EMSA). We also measured TNF-α, IL-1β, IL-6, and IgE concentration in the blood of AD mice by enzyme-linked immunosorbent assay (ELISA). TECA treatment attenuated the development of PA-induced atopic dermatitis. Histological analysis showed that TECA inhibited hyperkeratosis, mast cells and infiltration of inflammatory cells. TECA treatment inhibited expression of iNOS and COX-2, and NF-κB activity as well as the release of TNF-α, IL-1β, IL-6, and IgE. In addition, TECA (1, 2, 5 μg/mL) potently inhibited Lipopolysaccharide (LPS) (1 μg/mL)-induced NO production, expression of iNOS and COX-2, and NF-κB DNA binding activities in RAW264.7 macrophage cells. Our data demonstrated that TECA could be a promising agent for AD by inhibition of NF-κB signaling.

Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo

“…Molecules 2020, 25,892 3 of 20 Figure 1A,B shows a chromatogram of RECA and the reference standards which are asiaticoside, madecassoside, asiatic acid, and madecassic acid. Four triterpenes in RECA, which are madecassoside, asiaticoside, madecassic acid, and asiatic acid were quantified by using the HPLC gradient method with an ultraviolet (UV) detector at 200 nm (Table 1).…”

Medicinal plants in traumatic brain injury: Neuroprotective mechanisms revisited

“…33 The main components of C. asiatica are saponins, brahmoside, brahminoside, glycosides isothankuniside and hankuniside, sterols, and flavonoids. 172 This plant has been shown to have several pharmacological effects including antiinflammatory, 173 wound healing, 174 sedative, anxiolytic, 175 antidepressant, 176 anticonvulsant, 177 and antioxidant 178 activities.…”

Anti-oxidative effects of 17 β-estradiol and genistein in human skin fibroblasts and keratinocytes

“…While the constitutive NOS may act as a regulator of short-term and physiological phenomena and is involved in a small amount of NO release, the inducible NOS could be involved in longer-lasting cytotoxic and inflammatory immunological functions, by producing NO at higher extent [21,22]. NO has been reported to be associated with common forms of inflammatory skin diseases, thus representing a possible therapeutic target [23,24]. Moreover, it was also found to be over-secreted in response to UV irradiation by iNOS isoform [25].…”