Anti-inflammatory effect of the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden, the Tiger Milk mushroom

Lee, Sook Shien; Tan, Nget Hong; Fung, Shin Yee; Sim, Si Mui; Tan, Cher Siang; Ng, Szu Ting

doi:10.1186/1472-6882-14-359

Cited by 60 publications

(40 citation statements)

References 20 publications

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“…The cotton pellet-induced granuloma test is a model for studying the transudative and proliferative components of chronic inflammatory processes [26]. After several days of cotton pellet implantation, giant cells and undifferentiated connective tissue can be observed in addition to the fluid infiltration histologically.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and in-vitro antibacterial activities of Traditional Chinese Medicine Formula Qingdaisan

Zhao

Zhong

2016

BMC Complement Altern Med

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BackgroundQingdaisan (Formulated Indigo powder, QDS) are widely used for treatment of aphtha, sore throat and bleeding gums in China. The aim of the study is to evaluate the anti-inflammatory, antibacterial and dental ulcer therapeutic effects of QDS.MethodsDimethylbenzene-induced ear edema test and cotton pellet-induced granuloma test were used to evaluate anti-inflammatory activities of QDS on acute and chronic inflammatory. The healing time and local pathologic changes were used to assess the therapeutic effects of QDS on dental ulcer. The antibacterial activities of each component and the whole formulation of QDS were determined by agar well diffusion assay. High-dose and low-dose QDS were tested in this experiment and Gui Lin Watermelon Frost Powder (GLWFP) was used as positive control.ResultsOral treatment with QDS significantly accelerated the healing of ulcerative lesions induced by phenol injury. The dental ulcers of high-dose QDS group were all healed within 6 days. It was shorter than those of low-dose QDS group and GLWFP group. Less quantity of inflammatory cells and plenty fibroblasts were observed in pathological section of QDS groups. QDS also exhibited significant anti-inflammatory activity both in acute and chronic animal models. Although some of the components exhibited antibacterial activities, the whole formulation of QDS didn’t show any significant antibacterial activity in vitro.ConclusionThe study showed that QDS has obviously anti-inflammatory activity for both acute and chronic inflammatory, also has a remarkable effect for healing dental ulcer caused by phenol. QDS didn’t have antibacterial activity to selected strains in vitro.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and in-vitro antibacterial activities of Traditional Chinese Medicine Formula Qingdaisan

Zhao

Zhong

2016

BMC Complement Altern Med

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“…It has been used to treat gastric ulcers, chronic hepatitis and liver cancer ( Wong and Cheung, 2009 ). Previous studies demonstrated that the sclerotia of L. rhinocerotis exhibit anti-proliferative, anti-oxidant, anti-microbial, anti-inflammatory, and immunomodulatory effect as well as stimulatory effect on neurite outgrowth ( Lai et al, 2008 ; Wong et al, 2009 , 2011 ; Eik et al, 2012 ; Lee et al, 2012 ; Mohanarji et al, 2012 ; Lau et al, 2013 ; Phan et al, 2013 ; Yap et al, 2013 ; Zaila et al, 2013 ; Lee et al, 2014 ). Non-digestible carbohydrates (NDCs) from the sclerotia may function as novel prebiotics ( Gao et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Safety Evaluation of Sclerotium from a Medicinal Mushroom, Lignosus cameronensis (Cultivar): Preclinical Toxicology Studies

et al. 2017

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Twenty-eight days subacute toxicity studies performed in rats using sclerotial powder of Lignosus cameronensis cultivar was conducted to assess its safety for consumption prior to other scientific investigations on its medicinal benefits, nutraceutical or pharmaceutical application of the mushroom. The study was conducted at 250, 500, and 1000 mg/kg sclerotial powder of L. cameronensis cultivar (n = 5 for each respective dose, on both male and female groups) while control groups received only distilled water. At the end of the study (29th day), the animals were sacrificed followed by blood and organs collection for analysis. Subacute toxicity studies done shows that sclerotial powder of L. cameronensis cultivar at 250, 500, and 1000 mg/kg did not induce treatment related changes on behavioral patterns, gross physical appearance, growth pattern, body weight gain, values of hematological and clinical biochemical panels as well as histopathological findings on kidney, spleen, heart, lung and liver of the experimental rats. The no-observed-adverse-effect level dose for sclerotial powder of L. cameronensis cultivar in 28-days sub-acute toxicity study is determined to be 1000 mg/kg.

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“…6,7 The recent domestication of the mushroom has enabled assessment of the mushroom for antiproliferation activity against cancer cells, as well as its immunomodulatory, antioxidant, anti-inflammatory, antimicrobial, and neuritogenic activities. [8][9][10][11][12][13] The cultivated sclerotial powder at daily oral dose of up to 1,000 mg/kg was found to show no adverse effect on the growth rate, hematological and clinical biochemical parameters (including renal and liver function parameters) in animal studies. No pathological changes were found in the liver, kidney, heart, spleen, and lung of the treated animals.…”

Section: Introductionmentioning

confidence: 99%

Cloning, overexpression, purification, and modeling of a lectin (Rhinocelectin) with antiproliferative activity from Tiger Milk Mushroom, Lignosus rhinocerus

et al. 2019

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A lectin gene from the Tiger Milk Mushroom Lignosus rhinocerus TM02® was successfully cloned and expressed via vector pET28a in Escherichia coli BL21(DE3). The recombinant lectin, Rhinocelectin, with a predicted molecular mass of 22.8 kDa, was overexpressed in water‐soluble form without signal peptide and purified via native affinity chromatography Ni‐NTA agarose. Blast protein analysis indicated the lectin to be homologous to jacalin‐related plant lectin. In its native form, Rhinocelectin exists as a homo‐tetramer predicted with four chains of identical proteins consisting of 11 beta‐sheet structures with only one alpha‐helix structure. The antiproliferative activity of the Rhinocelectin against human cancer cell lines was concentration dependent and selective. The IC50 values against triple negative breast cancer cell lines MDA‐MB‐231 and breast cancer MCF‐7 are 36.52 ± 13.55 μg mL−1 and 53.11 ± 22.30 μg mL−1, respectively. Rhinocelectin is only mildly cytotoxic against the corresponding human nontumorigenic breast cell line 184B5 with IC50 value at 142.19 ± 36.34 μg mL−1. The IC50 against human lung cancer cell line A549 cells is 46.14 ± 7.42 μg mL−1 while against nontumorigenic lung cell line NL20 is 41.33 ± 7.43 μg mL−1. The standard anticancer drug, Doxorubicin exhibited IC50 values mostly below 1 μg mL−1 for the cell lines tested. Flow cytometry analysis showed the treated breast cancer cells were arrested at G0/G1 phase and apoptosis induced. Rhinocelectin agglutinated rat and rabbit erythrocytes at a minimal concentration of 3.125 μg mL−1 and 6.250 μg mL−1, respectively.

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Anti-inflammatory effect of the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden, the Tiger Milk mushroom

Cited by 60 publications

References 20 publications

Anti-inflammatory and in-vitro antibacterial activities of Traditional Chinese Medicine Formula Qingdaisan

Anti-inflammatory and in-vitro antibacterial activities of Traditional Chinese Medicine Formula Qingdaisan

Safety Evaluation of Sclerotium from a Medicinal Mushroom, Lignosus cameronensis (Cultivar): Preclinical Toxicology Studies

Cloning, overexpression, purification, and modeling of a lectin (Rhinocelectin) with antiproliferative activity from Tiger Milk Mushroom, Lignosus rhinocerus

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