Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells

Yoon, Weon‐Jong; Heo, Su Jin; Han, Sang‐Kyoo; Lee, Hye Ja; Kang, Gyeoung Jin; Kang, Hee Kyoung; Hyun, Jin Won; Koh, Young Sang; Yoo, Eun Sook

doi:10.1007/s12272-012-0812-5

Cited by 61 publications

(47 citation statements)

References 27 publications

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“…The MAPK pathways, containing three parallel kinase modules p38, JNK and ERK1/2, play a principal role in the regulation of iNOS and inflammatory cytokine production in RAW 264.7 cells exposed to LPS [35,36]. In general, the activation of p38 is required for the expression of a large number of inflammatory molecules.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of CDDO-Me on lipopolysaccharide-induced acute lung injury in mice

Chen

Mou

Tan

et al. 2015

International Immunopharmacology

146

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Section: Discussionmentioning

confidence: 99%

The protective effect of CDDO-Me on lipopolysaccharide-induced acute lung injury in mice

Chen

Mou

Tan

et al. 2015

International Immunopharmacology

146

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“…The results demonstrated that paeonol, M3, and M11 suppressed the high expression of iNOS protein in a dose-dependent manner, which strongly suggested that the decrease in NO production was due to the suppression of iNOS expression. Previous studies have shown that MAPK signaling pathway plays an important role in the regulation of iNOS and COX-2 expression, and in pro-inflammatory cytokine production in LPSactivated macrophages [23][24][25]. Our results indicated that paeonol, M3, and M11 strongly blocked the phosphorylation of MAPK/ERK and MAPK/p38 proteins but showed no obvious inhibition on the phosphorylation of MAPK/JNK protein, which suggested that the suppression on MAPK/ERK/p38 signal pathway may contribute to the anti-inflammatory effect of paeonol and its metabolites such as M3 or M11.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and Anti-oxidative Activities of Paeonol and Its Metabolites Through Blocking MAPK/ERK/p38 Signaling Pathway

et al. 2015

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The possible protective and curative effects of paeonol on carrageenan-induced acute hind paw edema in rats and dextran sulfate sodium (DSS)-induced colitis in mice have been evaluated. After oral administration, paeonol (20 and 40 mg/kg) reduced the edema increase in paw volumes and also the development of DSS-induced murine colitis. Furthermore, anti-inflammatory and anti-oxidant activities of paeonol (1) together with its 10 metabolites (M2~M11) were investigated by using in vitro anti-inflammatory and anti-oxidant assays. M3 and M11 exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities (with EC50 values of 93.44 and 23.24 μM, respectively). All the metabolites except M8 showed hydroxyl radical scavenging activities, and M3 and M11 were the most potent agents (with EC50 values of 336.02 and 124.05 μM, respectively). Inhibitory effects of paeonol, M2~M11 on the overproduction of nitric oxide (NO), and the release of TNF-α were also tested. M3 and M11 potently inhibited lipopolysaccharide (LPS)-induced overproduction of NO in macrophage RAW 264.7. Western blot results demonstrated that paeonol, M3, and M11 downregulated the high expression of inducible nitric oxide synthase (iNOS) and COX-2 proteins, and the effects of M3 and M11 were more potent when compared with paeonol. These findings indicated that paeonol may play anti-inflammatory and anti-oxidant roles by changing to its active metabolites after absorption. In addition, further investigations on the mechanism showed that paeonol, M3, and M11 blocked the phosphorylation of MAPK/ERK 1/2 and p38, whereas they showed no effect on the phosphorylation of JNK. The above results suggested that pre-treatment with paeonol might be an effective therapeutic intervention against inflammatory diseases including colitis.

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“…Anti-inflammatory activity has been reported for a range of other algal metabolites isolated from non-polar and DCM extracts including the chromene sargachromanol G from the Korean brown alga Sargassum siliquastrum (order Fucales) (Yoon et al 2012); halogenated compounds from the Malaysian red alga Laurencia snackeyi (Vairappan et al 2013) and the porphyrin derivatives, pheophorbide a and pheophytin a, along with the xanthophyll fucoxanthin, from the edible brown alga Saccharina japonica (order Laminariales) (Islam et al 2013). Other bioactive algal metabolites isolated from non-polar extracts include cytotoxic, antibacterial linear sesquiterpenoids from the green alga Penicillus capitatus (class Ulvophyceae, order Bryopsidales) (Paul and Fenical 1984); antibacterial labdane diterpenoids from Ulva fasciata (Chakraborty et al 2010); and antiatherosclerotic phytosterols from the brown alga Sargassum fusiforme (order Fucules) (Chen et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity

et al. 2014

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Increasingly, macroalgae are being recognised as a growth opportunity for functional foods and nutritional security in the future. Dominating traits of interest are metabolites that function as anti-inflammatories and are antiproliferative. However, seaweeds from the northern hemisphere dominate this field of research. Australia has a unique flora of macroalgae, and it is poorly understood which species should be targeted for cultivation towards food and health markets. Here, six Australian marine macroalgae were selected for screening of one anti-inflammatory group; n-3 polyunsaturated fatty acids (PUFA). PUFA profiles were determined using gas chromatography-mass spectrometry and multivariate analysis. Thirty-one fatty acids (FA) were identified across the six macroalgal species with C16:0 the dominant FA in all samples, variations across taxa in the saturated FA C10:0, C14:0, C16:0, C18:0 and C20:0 and variations in monounsaturated FA attributed to C16:1 n-7 and C18:1 n-9. For PUFA profiles, all six species had significantly different n-6/n-3 ratios, while the green seaweed Ulva species possessed the lowest n-6/n-3 ratio of 0.4, along with a 2-fold higher C18:3 n-3 to C18:2 n-6 content. Ulva sp. was the only species that contained docosahexaenoic acid. Extracts of both the Ulva sp. and Hormosira banksii showed selective cytotoxicity towards a human pancreatic cancer cell line, while the nonpolar extracts of all six algae species strongly inhibited production of the inflammatory-mediator nitric oxide. Abstract Increasingly macroalgae are being recognized as a growth opportunity for functional foods and nutritional security in the future. Dominating traits of interest are metabolites that function as anti-inflammatories and are antiproliferative. However seaweeds cultivated in the northern hemisphere dominate this field of research. Australia has a unique flora of macroalgae and it is poorly understood which species should be targeted for cultivation towards food and health markets. Herein, six Australian marine macroalgae were selected for screening of one antiinflammatory group; n-3 polyunsaturated fatty acids (PUFA). PUFA profiles were determined using gas chromatography -mass spectrometry and multivariate analysis. Thirty-one fatty acids (FA) were identified across the six macroalgal species with C16:0 the dominant FA in all samples, variations across taxa in the saturated FA C10:0, C14:0, C16:0, C18:0 and C20:0 and variations in monounsaturated FA attributed to C16:1 n-7 and C18:1 n-9. For PUFA profiles, all six species had significantly different n-6/n-3 ratios, while the green seaweed Ulva sp. possessed the lowest n-6/n-3 ratio of 0.4, along with a 2-fold higher C18:3 n-3 to C18:2 n-6 content. Ulva sp. was the only species that contained docosahexaenoic acid. Extracts of both the Ulva sp. and H. banksii showed selective cytotoxicity towards a human pancreatic cancer cell line, while the non-polar extracts of all six algal species strongly inhibited production of the inflammatory-mediator nitric ...

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Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells

Cited by 61 publications

References 27 publications

The protective effect of CDDO-Me on lipopolysaccharide-induced acute lung injury in mice

The protective effect of CDDO-Me on lipopolysaccharide-induced acute lung injury in mice

Anti-inflammatory and Anti-oxidative Activities of Paeonol and Its Metabolites Through Blocking MAPK/ERK/p38 Signaling Pathway

Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity

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