Anti-inflammatory effect of resveratrol on TNF-α-induced MCP-1 expression in adipocytes

Zhu, Jian; Wei, Yong; Wu, Xiaohong; Yu, Ying; Lv, Jinghuan; Liu, Cuiping; Mao, Xiaodong; Zhu, Yunxia; Xu, Kuanfeng; Han, Xiao; Liu, Chao

doi:10.1016/j.bbrc.2008.02.034

Cited by 136 publications

(88 citation statements)

References 24 publications

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“…10 New results showed how RSV effectively inhibits TNF-a-induced secretion of MCP-1, IL6 and PAI, while restoring the decreased adiponectin secretion, in 3T3-L1 adipocytes. 32,33 In this respect, Sirt1 activation posed anti-inflammatory effect, by decreasing TNF-a-induced NF-kB activity in 3T3-L1 adipocytes. 33 In addition, recent findings illustrate how Sirt1 in a non-adipose human cell line, modulates NF-kB transcription, by deacetylating the Re1/p65 subunit of NF-kB at lysine 310 subunit.…”

Section: Discussionmentioning

confidence: 99%

“…This concentration level is well in line with other studies using different cell types, where 50 mM RSV is most often used. 33,34 In addition, a time-course analysis was performed (6-72 h), showing that using 24-h incubations were optimal in showing effects of RSV (data not shown).…”

Section: Concentration Responsementioning

confidence: 99%

“…Recently, studies have revealed that RSV inhibits the production of the proinflammatory adipokines TNF-a, MCP-1, IL6, PAI-1 and increases adiponectin production in 3T3-L1 cells. 32,33 Earlier studies with RSV and adipose tissue have mainly been performed in rodents or in cell cultures. However, we recently showed that Sirt1 is expressed in human adipose tissue, and Sirt1 expression in human adipose tissue was also upregulated by severe CR.…”

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory effect of resveratrol on adipokine expression and secretion in human adipose tissue explants

et al. 2010

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Objective: Human obesity is closely associated with a state of chronic low-grade inflammation, which also involves the adipose tissue with enhanced production of bioactive substances (adipokines). Calorie restriction (CR) reduces adipocytokine production and improves metabolic profile in rodents. Some of these effects are mediated through activation of the sirtuin 1 (Sirt1) enzyme, and in this study, we investigate whether the natural phytoalexin, resveratrol (RSV), which is a potent Sirt1 activator, has anti-inflammatory effects in human adipose tissue explants. Design: The effect of RSV on interleukin 1b (IL1b)-induced change of adipokine mRNA gene expression and secretion were measured in human adipose tissue explants. Results: Exposure of human adipose tissue in vitro to IL1b for 24 h increased secretion of the proinflammatory adipokines IL6, IL8 and monocyte chemoattractant protein 1 (MCP-1) 3-7.7-fold (Po0.05) and increased IL6, IL8, MCP-1, IL1b and PAI-1 mRNA expression 1.3-7.2-fold (Po0.05) accordingly. Concomitant incubations with RSV reversed the IL1b-stimulated secretion (16-36%) and gene expression (25-48%) of these adipokines. IL1b reduced adiponectin mRNA expression (40%), a decrement that was reversed by RSV treatment. Similar effects were observed in differentiated human preadipocytes in primary culture, indicating that human adipocytes are a potential target for RSV effects. Finally, the effects were neutralized by sirtinol, a Sirt1 inhibitor. Conclusion: This study is the first to show anti-inflammatory effects of RSV on adipokine expression and secretion in human adipose tissue in vitro through the SIRT1 pathway. Thus, RSV is hypothesized to possess beneficial effects and might improve the metabolic profile in human obesity.

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Section: Discussionmentioning

confidence: 99%

Section: Concentration Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory effect of resveratrol on adipokine expression and secretion in human adipose tissue explants

et al. 2010

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“…Because CXCL2 and MCP-1 expression are regulated by NF-B, 32 we studied whether TNF-␣-or LPS-induced NF-B activation is impaired in RTECs from FAT10 Ϫ/Ϫ mice. An NF-B reporter plasmid (pGL2/NF-B luciferase) was transfected into FAT10 ϩ/ϩ and FAT10 Ϫ/Ϫ RTECs for 24 h, and cells were assayed for luciferase activity after additional incubation with TNF-␣ or LPS (Invitrogen) for 16 h. A plasmid encoding renilla luciferase was co-transfected as an internal control for transfection efficiency.…”

Section: Tnf-␣-and Lps-induced Nf-b Activation In Rtecs Is Dependent mentioning

confidence: 99%

The Ubiquitin-Like Protein FAT10 Mediates NF-κB Activation

Gong¹,

Canaan

Wang³

et al. 2010

Journal of the American Society of Nephrology

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“…RSV has been shown to inhibit the hepatic carcinogenactivating enzymes, including cytochrome CYP3A/2 P450 and 1A1 (CYP1A1) and induce hepatic phase 2 conjugating enzymes, namely glutathione S-transferase (GST), UDP-glucuronosyl transferase and NAD(P)H: quinine oxidoreductase, in vivo and vitro [101,102]. The effects of these enzyme modulations by RSV could be the reduced exposure of cells to carcinogens due to inhibition of carcinogen activation, elevated carcinogen detoxification and elimination [102].…”

Section: Resveratrolmentioning

confidence: 99%

Reduction of Hepatotoxicity Induced by Doxorubicin

Bengaied¹,

Ribeiro²,

Amri³

et al. 2017

J Integr Oncol

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Doxorubicin (DOX) has been used in the treatment of variety of cancers but its administration is limited by a dosedependent toxicity. Its cytotoxic effects on malignant cells have shown an increase in the risk of cardiotoxicity, hepatoxicity, renal insufisance.Antioxydants have been explored for both their cancer preventive properties and chemodulatory of DOX toxicity. Resveratrol (RSV) is a polyphenolic constituent of several dietary mainly of grapes and wine origin recently its anticancer potential has been extensively explored, revealing its anti-proliferative effect on different cancer cell lines, both in vitro and in vivo. RSV is also known to have modulatory effects on cell apoptosis, migration and growth via various signaling pathways. Though, RSV possesses great medicinal value, its applications as a therapeutic drug is limited. Problems like low oral bioavailability and poor aqueous solubility make RSV an unreliable candidate for therapeutic purposes. Additionally, the rapid gastrointestinal digestion of RSV is also a major barrier for its clinical translation. Hence, to overcome these disadvantages RSV-based nanodelivery systems have been considered in recent times. Nanodelivery systems of RSV have shown promising results in its uptake by the epithelial system as well as enhanced delivery to the target site. Herein we have tried to bring new new insights into the molecular mechanisms of DOX toxicity with respect to DNA damage, free radicals and whether RSV can be a playmaker as chemodulatory of DO x .

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Anti-inflammatory effect of resveratrol on TNF-α-induced MCP-1 expression in adipocytes

Cited by 136 publications

References 24 publications

Anti-inflammatory effect of resveratrol on adipokine expression and secretion in human adipose tissue explants

Anti-inflammatory effect of resveratrol on adipokine expression and secretion in human adipose tissue explants

The Ubiquitin-Like Protein FAT10 Mediates NF-κB Activation

Reduction of Hepatotoxicity Induced by Doxorubicin

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