Anti-Inflammatory Effect of Qingpeng Ointment in Atopic Dermatitis-Like Murine Model

Abstract

Qingpeng ointment (QP) is a Chinese medicine which has been used in treatment of atopic dermatitis (AD) in China. AD-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene (DNFB) on shaved backs. The mice were then treated for 2 weeks with QP of different concentrations and Mometasone Furoate cream (MF), respectively. Macroscopic and microscopic changes of the skin lesions were observed after the treatment. The levels of serum immunoglobulin (Ig) E, tissue interferon (IFN)-γ, and interleukin (IL)-4 and IL-17A and the levels of involucrin, filaggrin, and kallikrein7 in epidermis were measured. The results show severe dermatitis with immune profiles similar to human acute AD. A significant infiltration of CD4+ T and mast cells was observed in dermis of lesion but inhibited by QP after a 2-week treatment with it. The production of IgE, IL-4 and the mRNA expression of IL-17A were also suppressed, but the level of IFN-γ was increased. MF suppressed all production of these cytokines and IgE. Accordingly, the mechanism of QP on AD might correlate with its ability of modulating the immune dysfunctions rather than suppressing them. It had no effect on expressions of involucrin and filaggrin, except that its vehicle decreased the level of kallikrein7.

Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model

“…AD is a complex allergic condition that is characterized by high IgE levels, and IgE-mediated mechanisms are thought to play an important role in the pathogenesis of this disease [ 1 ]. In the present study, we applied DNFB as a hapten to BALB/c mice for 5 weeks to induce dermatitis, with an immune profile of higher levels of IgE, which was similar to the early reaction of AD [ 29 ].…”

“…The BALB/c mice were divided into two groups, namely the AD group (n = 10) and the control group (n = 10). DNFB (Wako, Japan) diluted in the mixture of acetone and olive oil (4:1) was used for sensitization [ 29 ]. The dorsal side of the mice was shaved on the first week of the experiment.…”

“…The thickness of the dorsal skin and the length of the spleen were measured by using a micrometer (Hautine International Co, China) before and after the mice were sacrificed, respectively (n = 10). Three different sites of the back skin were measured by two volunteers and averages were calculated [ 29 ].…”

The Effect of the Polygonum tinctoria Niram on Atopic Dermatitis in Dinitrochlorobenzene-Induced BALB/c Mice

“…This staining allows observing both rodent and human connective tissues; purple colored, metachromatic staining reactive cells are observed and evaluated (Lee et al, 2006). It was previously demonstrated that decreased in numbers of mast cells might be due to efficacy of therapeutic agents (Ahn et al, 2009;Nagai & Okunishi, 2009;Li et al, 2010;Zheng et al, 2011;Li et al, 2013). As we demonstrated, PTC significantly lowered the numbers of mast cells in mice dorsal skin, suggesting its suppressing potential against AD like symptoms and providing a possibility to utilize PTN as a raw material for the development of therapeutic agents.…”

Resveratrol ameliorates 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesions through effects on the epithelium

“…The induction of AD by using DNFB was established based on previous research ( Li et al, 2013 ). DNFB was purchased from Sigma Chemical (St. Louis, MO, USA) and dissolved in a mixture of acetone and olive oil (4:1).…”