Anti-inflammatory effect of methanol extract from<i>Erigeron Canadensis</i>L. may be involved with upregulation of heme oxygenase-1 expression and suppression of NFκB and MAPKs activation in macrophages

Sung, Jeehye; Sung, Myongsoon; Kim, Young-Hwa; Ham, Hyeonmi; Jeong, Heon‐Sang; Lee, Junsoo

doi:10.4162/nrp.2014.8.4.352

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…In fact, inhibition of NF-κB p65 activation LPS-induced was achieved through SP treatment indicating an anti-inflammatory effect of SP. Accordingly, other authors found that anti-inflammatory effects on RAW 264.7 of the molecules are exerted through NF-κB suppression [38]. NF-κB is also directly involved in pro-inflammatory responses such as adhesion and migration of leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Substance P Induces HO-1 Expression in RAW 264.7 Cells Promoting Switch towards M2-Like Macrophages

Montana

Lampiasi

2016

PLoS ONE

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Substance P (SP) is a neuropeptide that mediates many physiological as well as inflammatory responses. Recently, SP has been implicated in the resolution of inflammation through induction of M2 macrophages phenotype. The shift between M1-like and M2-like, allowing the resolution of inflammatory processes, also takes place by means of hemeoxygenase-1 (HO-1). HO-1 is induced in response to oxidative stress and inflammatory stimuli and modulates the immune response through macrophages polarisation. SP induces HO-1 expression in human periodontal ligament (PDL), the latter potentially plays a role in cytoprotection. We demonstrated that SP promotes M2-like phenotype from resting as well as from M1 macrophages. Indeed, SP triggers the production of interleukine-10 (IL-10), interleukine-4 (IL-4) and arginase-1 (Arg1) without nitric oxide (NO) generation. In addition, SP increases HO-1 expression in a dose- and time-dependent manner. Here we report that SP, without affecting cell viability, significantly reduces the production of pro-inflammatory cytokines and enzymes, such as tumor necrosis factor-alpha (TNF-α), interleukine-6 (IL-6), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and ameliorates migration and phagocytic properties in LPS-stimulated RAW 264.7 cells. M2-like conversion required retention of NF-κB p65 into the cytoplasm and HO-1 induced expression. Silencing of the HO-1 mRNA expression reversed the induction of pro-inflammatory cytokines in RAW 264.7 stimulated by LPS and down-regulated anti-inflammatory hallmarks of M2 phenotype. In conclusion, our data show that SP treatment might be associated with anti-inflammatory effects in LPS-stimulated RAW 264.7 cells by suppressing NF-κB activation and inducing HO-1 expression.

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Section: Discussionmentioning

confidence: 99%

Substance P Induces HO-1 Expression in RAW 264.7 Cells Promoting Switch towards M2-Like Macrophages

Montana

Lampiasi

2016

PLoS ONE

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“…Thus, LPS acts as an inducer of lung injury and it is used to establish a model of ALI [1,36]. In an experimental murine model of ALI, LPS caused damage to endothelial and epithelial barriers and activated neutrophils and macrophages, thereby releasing various pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6 [37][38][39]. It has been reported that these pro-inflammatory cytokines activate and recruit neutrophils to cross the vascular endothelium into the lung and cause a cascading inflammatory response to amplify tissue injury [11,13,40].…”

Section: Discussionmentioning

confidence: 99%

Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Lee

Shin

et al. 2020

Antioxidants

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Spiraea prunifolia var. simpliciflora (SP) is traditionally used as an herbal remedy to treat fever, malaria, and emesis. This study aimed to evaluate the anti-oxidative and anti-inflammatory properties of the methanol extract of SP leaves in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. SP decreased the number of inflammatory cells and the levels of TNF-α, interleukin (IL)-1β, and IL-6 in the bronchoalveolar lavage fluid, and inflammatory cell infiltration in the lung tissues of SP-treated mice. In addition, SP significantly suppressed the mRNA and protein levels of TNF-α, IL-1β, and IL-6 in TNF-α-stimulated NCI-H292 cells. SP significantly suppressed the phosphorylation of the mitogen-activated protein kinases (MAPKs) and p65-nuclear factor-kappa B (NF-κB) in LPS-induced ALI mice and TNF-α-stimulated NCI-H292 cells. SP treatment enhanced the nuclear translocation of nuclear factor erythroid 2-related factor (Nrf2) with upregulated antioxidant enzymes and suppressed reactive oxygen species (ROS)-mediated oxidative stress in the lung tissues of LPS-induced ALI model and TNF-α-stimulated NCI-H292 cells. Collectively, SP effectively inhibited airway inflammation and ROS-mediated oxidative stress, which was closely related to its ability to induce activation of Nrf2 and inhibit the phosphorylation of MAPKs and NF-κB. These findings suggest that SP has therapeutic potential for the treatment of ALI.

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“…In addition, EA11 also upregulates the expression of PPAR- γ , another recognized anti-inflammatory mediator [ 19 , 20 ] ( Figure 3(c) ). Several natural products have been demonstrated the induction of HO-1 expression in macrophages and thus eliminate the expression of proinflammatory mediators [ 21 , 22 ]. EA11 has added another into this natural product list.…”

Section: Discussionmentioning

confidence: 99%

Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti‐Inflammatory Effects by Regulating miR‐155 Expression and JNK Signaling Pathway

Chen

Jiang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Hedyotis diffusa Willd and Scutellaria barbata D. Don (HDSB) were the core couplet in medicines that were commonly used for the purpose of anti-inflammation and anticancer treatments in China. However, biological properties of this couplet have not been fully elucidated. In this study, we screened fractions of HDSB for their anti-inflammatory activities and explored pertinent molecular mechanisms on murine macrophage RAW264.7 cell model. Ethyl acetate fraction from the aqueous extract of the couplet at equal weight ratio (EA11) showed the strongest inhibition of the nitrite accumulation in supernatant of RAW264.7 cells stimulated with lipopolysaccharide (LPS)/interferon-γ (IFN-γ). In addition, EA11 inhibited iNOS and IL-1β expression in a concentration-dependent manner while promoting the expression of HO-1 and PPAR-γ. Anti-inflammatory capability is most likely facilitated by its inhibitory effect on JNK signaling pathway and miR-155 expression. This study suggests that EA11 may be represented as a potential anti-inflammatory therapeutic candidate.

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Anti-inflammatory effect of methanol extract fromErigeron CanadensisL. may be involved with upregulation of heme oxygenase-1 expression and suppression of NFκB and MAPKs activation in macrophages

Cited by 14 publications

References 31 publications

Substance P Induces HO-1 Expression in RAW 264.7 Cells Promoting Switch towards M2-Like Macrophages

Substance P Induces HO-1 Expression in RAW 264.7 Cells Promoting Switch towards M2-Like Macrophages

Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti‐Inflammatory Effects by Regulating miR‐155 Expression and JNK Signaling Pathway

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