Anti-Inflammatory Effect ofTrachysalambria curvirostrisExtract on a DSS-Induced Mouse Colitis Model

Abstract

Trachysalambria curvirostris is a crustacean found near the southwest coast of Korea which contains various nutrients such as iron, calcium, taurine, and chitosan. In particular, chitosan has anti-inflammatory, antibacterial, anticancer, and antioxidant effects. The purpose of this study was to evaluate the anti-inflammatory effect of the extract of Trachysalambria curvirostris on inflammatory bowel disease (IBD). Inflammatory factors (COX-2, IL-1β, IL-6, iNOS, TNF-α) in cells and colon tissue induced by lipopolysaccharide (LPS) and dextran sulfate sodium (DSS) were confirmed using RT-PCR. The generation of cytokines (IL-6, TNF-α) in the colon tissue protein of ICR mice caused by DSS was measured by ELISA. Compared to the LPS and DSS groups, the expression level of inflammatory cytokines showed a reduction in the group treated with the Trachysalambria curvirostris extract. Similarly, the ELISA results showed that the expression level of inflammatory cytokine decreased compared to the DSS group. As a result of H&E staining using mouse colon tissue, it was possible to observe a lower invasion of inflammatory cells in the Trachysalambria curvirostris treated group than in the DSS group. It is necessary to conduct additional research on the exact mechanism of reaction to identify the active components in the Trachysalambria curvirostris extract, and to prove the value of the Trachysalambria curvirostris for IBD prevention and treatment.