Anti-Inflammatory Effect of Heme Oxygenase-1 Toward<i>Porphyromonas gingivalis</i>Lipopolysaccharide in Macrophages Exposed to Gomisins A, G, and J

Ryu, Eun Yeon; Park, Sun Young; Kim, Sun Gun; Park, Da Jung; Kang, Jum-Soon; Kim, Young Hun; Seetharaman, Rajasekar; Choi, Yang-Ho; Lee, Sang-Joon

doi:10.1089/jmf.2011.1656

Cited by 16 publications

(13 citation statements)

References 30 publications

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“…Cox2 mRNA expression in RAW264.7 cells stimulated with cinnamates and (meth)acrylates. It has been well documented that the inflammatory response to E. coli and P. gingivalis LPS or P. gingivalis fimbriae involves the production of COX2, NOS2 and proinflammatory cytokines such as TNFα, interleukin (IL)-6, and IL1β in RAW264.7 cells (28,30,40). Contrary to the pro-inflammatory effects of LPS, the inflammatory mediators COX2 and NOS2 and cytokines such as TNFα at both the protein and gene levels may also be expressed in macrophages stimulated by α,βunsaturated carbonyl compounds.…”

Section: Resultsmentioning

confidence: 99%

“…in P. gingivalis LPS-stimulated gingival fibroblasts and RAW264.7 cells may be one way to screen anti-inflammatory antioxidant agents for their effects on age-related chronic diseases such as periodontitis with systemic problems. In this context, HO1 expression may be useful as a target for antiinflammatory antioxidant drugs and preventive agents against such age-related chronic diseases such as periodontitis (28)(29)(30)(31). We previously reported that the inhibitory effects of phenylpropanoids, including eugenol, bis-eugenol (the orthodimer of eugenol), magnolol, honokiol and curcumin on Escherichia coli LPS-induced RAW264.7 cells in terms of the release of pro-inflammatory mediators and cytokines, suggested that these compounds had potent anti-inflammatory activities (30,31).…”

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confidence: 99%

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Cytotoxicity and Pro-/Anti-inflammatory Properties of Cinnamates, Acrylates and Methacrylates Against RAW264.7 Cells

Murakami

Kawata

Suzuki

et al. 2018

In Vivo

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Background/Aim: Periodontitis is a chronic inflammatory disease linked to various systemic age-related conditions. It is known that α,β-unsaturated carbonyl compounds such as dietary cinnamates (β-phenyl acrylates) and related (meth)acrylates can have various positive and negative health effects, including cytotoxicity, allergic activity, pro-and anti-inflammatory activity, and anticancer activity. To clarify the anti-inflammatory properties of α,β-unsaturated carbonyl compounds without a phenolic group in the context of periodontal tissue inflammation and alveolar bone loss, we investigated the cytotoxicity and up-regulatory/downregulatory effect of three trans-cinnamates (trans-cinnamic acid, methyl cinnamate, trans-cinnamaldehyde), two acrylates (ethyl acrylate, 2-hydroxyethyl acrylate), and three methacrylates (methyl methacrylate, 2-hydroxyethyl methacrylate, and triethyleneglycol dimethacrylate) using RAW264.7 cells. Materials and Methods: Cytotoxicity was determined using a cell counting kit (CCK-8) and mRNA expression was determined using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Proinflammatory and anti-inflammatory properties were assessed in terms of expression of mRNAs for cyclo-oxygenase-2 (Cox2), nitric oxide synthase 2 (Nos2), tumor necrosis factoralpha (Tnfa) and heme oxygenase 1 (Ho1). Results: The most cytotoxic compound was 2-hydroxyethyl acrylate, followed by ethyl acrylate and cinnamaldehyde (50% lethal cytotoxic concentration, LC 50 =0.2-0.5 mM). Cox2 mRNA expression was up-regulated by cinnamaldehyde and 2-hydroxyethyl acrylate, particularly by the former. In contrast, the upregulatory effect on Nos2 mRNA expression was in the order: cinnamaldehyde >> ethyl acrylate ≈ triethyleneglycol dimethacrylate >> methyl methacrylate ≈ methyl cinnamate. On the other hand, cinnamic acid and 2-hydroxyethyl methacrylate had no effect on gene expression. The two acrylates, but not cinnamates and methacrylates, up-regulated the expression of Ho1 mRNA at a non-cytotoxic concentration of 0.1 mM. Expression of Cox2, Nos2 and Tnfa mRNAs induced by Porphyromonas gingivalis lipopolysaccharide was greatly suppressed by cinnamaldehyde, methyl cinnamate and the two acrylates at 0.1 mM (p<0.05), and slightly, but significantly suppressed by cinnamic acid and methacrylates at 0.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Cytotoxicity and Pro-/Anti-inflammatory Properties of Cinnamates, Acrylates and Methacrylates Against RAW264.7 Cells

Murakami

Kawata

Suzuki

et al. 2018

In Vivo

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“…Because antigen-presenting cells (APCs), such as dendritic cells (DCs), monocytes and macrophages express high levels of HO-1, the function and antiinflammatory capacity of this molecule have been extensively studied. [24][25][26] A better understanding of the biology and function for HO-1 within these APCs could contribute to the design of improved anti-inflammatory ther-apies. In this article, we review recent findings for the role of HO-1 in the modulation of immunity.…”

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confidence: 99%

Modulation of antigen processing by haem‐oxygenase 1. Implications on inflammation and tolerance

et al. 2016

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SummaryHaem-oxygenase-1 (HO-1) is an enzyme responsible for the degradation of haem that can suppress inflammation, through the production of carbon monoxide (CO). It has been shown in several experimental models that genetic and pharmacological induction of HO-1, as well as non-toxic administration of CO, can reduce inflammatory diseases, such as endotoxic shock, type 1 diabetes and graft rejection. Recently, it was shown that the HO-1/CO system can alter the function of antigen-presenting cells (APCs) and reduce T-cell priming, which can be beneficial during immune-driven inflammatory diseases. The molecular mechanisms by which the HO-1 and CO reduce both APC-and T-cell-driven immunity are just beginning to be elucidated. In this article we discuss recent findings related to the immune regulatory capacity of HO-1 and CO at the level of recognition of pathogen-associated molecular patterns and T-cell priming by APCs. Finally, we propose a possible regulatory role for HO-1 and CO over the recently described mitochondria-dependent immunity. These concepts could contribute to the design of new therapeutic tools for inflammation-based diseases.

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“…It has been well documented that the inflammatory response to Eschericha coli and P. gingivalis LPS or P. gingivalis fimbriae involves the production of Cox2, Nos2 and proinflammatory cytokines such as tumor necrosis factor (Tnf)-α, interleukin (IL)-6, and IL-1β in RAW264.7 cells (15,16,31). In addition, anti-inflammatory properties of Ho-1 enzyme toward P. gingivalis LPS have been investigated (32).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Pro-inflammatory Properties of Aliphatic Alpha, Beta-unsaturated Acid and Ester Monomers in RAW264.7 Cells and Their Chemical Reactivity

Murakami

Kawata

Suzuki

et al. 2019

In Vivo

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Background/Aim: α,β-Unsaturated ester monomers such as methyl methacrylates (MMA), 2-hydroxyethyl methacrylates (2-HEMA), ethyleneglycol dimethacrylate (EGDMA) and triethyleneglycol dimetacrylate (TEGDMA) have been widely used in dentistry as dental materials. The present study was designed to clarify the proinflammatory activity of monomers. Materials and Methods: The cytotoxicity of the monomers and their effects on the expression of cyclooxygenase-2 (Cox2), nitric oxide synthase 2 (Nos2) and heme oxygenase 1 (Ho-1) mRNAs in RAW264.7 cells were determined using a cell counting kit and real-time reverse transcriptase-polymerase chain reaction, respectively. Results: The cytotoxicity declined in the order n-butyl acrylate (nBA) > acrylic acid > TEGDMA > EGDMA > methacrylic acid ≈ 2-HEMA > lauryl methacrylate > nBMA > MMA. nBA and EGDMA at 1 mM up-regulated the expression of Cox2 mRNA. In contrast, 1 mM nBA and 10 mM 2-HEMA up-regulated the expression of Nos2 mRNA. Up-regulation of Ho-1 mRNA expression was found for 0.1 mM nBA, 1 mM EGDMA and 2 mM TEGDMA. The electrophilicity, ω was calculated on the basis of the density function theory BLYP/6-31G*. Conclusion: nBA and EGDMA with high ω values exerted potent proinflammatory activities. nBA, EGDMA and TEGDMA upregulated Ho-1 gene expression. Ho-1 gene activation of monomers may promote resistance of chemical carcinogenesis in biological systems.

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Anti-Inflammatory Effect of Heme Oxygenase-1 TowardPorphyromonas gingivalisLipopolysaccharide in Macrophages Exposed to Gomisins A, G, and J

Cited by 16 publications

References 30 publications

Cytotoxicity and Pro-/Anti-inflammatory Properties of Cinnamates, Acrylates and Methacrylates Against RAW264.7 Cells

Cytotoxicity and Pro-/Anti-inflammatory Properties of Cinnamates, Acrylates and Methacrylates Against RAW264.7 Cells

Modulation of antigen processing by haem‐oxygenase 1. Implications on inflammation and tolerance

Cytotoxicity and Pro-inflammatory Properties of Aliphatic Alpha, Beta-unsaturated Acid and Ester Monomers in RAW264.7 Cells and Their Chemical Reactivity

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